Influence of α-1,3-glucan synthase gene agsE on protoplast formation for transformation of Aspergillus luchuensis

Tokashiki, Jikian; Hayashi, Risa; Yano, Shigekazu; Watanabe, Taisuke; Toyama, Hirohide; Mizutani, Osamu

doi:10.1016/j.jbiosc.2019.01.018

Cited by 16 publications

(7 citation statements)

References 18 publications

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“…Hyphal pellets in Δ ags1 that are smaller than those in the AfS35 strain of A. fumigatus ( Fig. 1A ) support the role of α-1,3-glucan in hyphal aggregation and are consistent with the phenotypes of α-1,3-glucan-deficient strains of A. oryzae , A. nidulans , and Aspergillus niger ( 22 , 23 , 25 , 36 , 37 ). In the Δ ags1 Δ gtb3 strain, AS2-Glc was scarcely detected ( Fig.…”

Section: Discussionsupporting

confidence: 69%

Quantitative Monitoring of Mycelial Growth of Aspergillus fumigatus in Liquid Culture by Optical Density

et al. 2022

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Section: Discussionsupporting

confidence: 69%

Quantitative Monitoring of Mycelial Growth of Aspergillus fumigatus in Liquid Culture by Optical Density

et al. 2022

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“…In A. niger, the expression of agsA (orthologous to A. fumigatus AGS3; no ortholog in A. nidulans) and agsE (orthologous to A. nidulans agsB) is upregulated in the presence of stress-inducing compounds in the cell wall (Damveld et al, 2005). In the kuro (black) koji mold Aspergillus luchuensis, disruption of agsE (orthologous to A. nidulans agsB) improves the protoplast formation (Tokashiki et al, 2019). Recently Uechi et al revealed that A. luchuensis agsB (no ortholog in A. nidulans) plays a role in nigeran synthesis (Uechi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

A Glycosylphosphatidylinositol-Anchored α-Amylase Encoded by amyD Contributes to a Decrease in the Molecular Mass of Cell Wall α-1,3-Glucan in Aspergillus nidulans

Miyazawa

Yamashita

Takeuchi

et al. 2022

Front. Fungal Biol.

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α-1,3-Glucan is one of the main polysaccharides in the cell wall of Aspergillus nidulans. We previously revealed that it plays a role in hyphal aggregation in liquid culture, and that its molecular mass (MM) in an agsA-overexpressing (agsAOE) strain was larger than that in an agsB-overexpressing (agsBOE) strain. The mechanism that regulates its MM is poorly understood. Although the gene amyD, which encodes glycosylphosphatidylinositol (GPI)-anchored α-amylase (AmyD), is involved in the biosynthesis of α-1,3-glucan in A. nidulans, how it regulates this biosynthesis remains unclear. Here we constructed strains with disrupted amyD (ΔamyD) or overexpressed amyD (amyDOE) in the genetic background of the ABPU1 (wild-type), agsAOE, or agsBOE strain, and characterized the chemical structure of α-1,3-glucans in the cell wall of each strain, focusing on their MM. The MM of α-1,3-glucan from the agsBOEamyDOE strain was smaller than that in the parental agsBOE strain. In addition, the MM of α-1,3-glucan from the agsAOE ΔamyD strain was greater than that in the agsAOE strain. These results suggest that AmyD is involved in decreasing the MM of α-1,3-glucan. We also found that the C-terminal GPI-anchoring region is important for these functions.

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“…In comparison with the parental strain, the α-1,3-glucan-deficient mutant of A. nidulans showed the same levels of radial growth and conidiation on agar plates [27], and its mycelial weight in liquid culture was higher [67]. In the kuro (black) koji mold Aspergillus luchuensis, although an agsE disruption mutant formed hyphal pellets, their average diameter was lower than in the wild-type strain in shake-flask culture [68]. The hyphae of wild-type A. oryzae form pellets similar to those of wild-type A. nidulans in shakeflask culture [27,67].…”

Section: α-13-glucanmentioning

confidence: 94%

The mechanisms of hyphal pellet formation mediated by polysaccharides, α-1,3-glucan and galactosaminogalactan, in Aspergillus species

Miyazawa

Yoshimi

Abe

2020

Fungal Biol Biotechnol

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Filamentous fungi are widely used for production of enzymes and chemicals, and are industrially cultivated both in liquid and solid cultures. Submerged culture is often used as liquid culture for filamentous fungi. In submerged culture, filamentous fungi show diverse macromorphology such as hyphal pellets and dispersed hyphae depending on culture conditions and genetic backgrounds of fungal strains. Although the macromorphology greatly affects the productivity of submerged cultures, the specific cellular components needed for hyphal aggregation after conidial germination have not been characterized. Recently we reported that the primary cell wall polysaccharide α-1,3glucan and the extracellular polysaccharide galactosaminogalactan (GAG) contribute to hyphal aggregation in Aspergillus oryzae, and that a strain deficient in both α-1,3-glucan and GAG shows dispersed hyphae in liquid culture. In this review, we summarize our current understanding of the contribution of chemical properties of α-1,3-glucan and GAG to hyphal aggregation. Various ascomycetes and basidiomycetes have α-1,3-glucan synthase gene(s). In addition, some Pezizomycotina fungi, including species used in the fermentation industry, also have GAG biosynthetic genes. We also review here the known mechanisms of biosynthesis of α-1,3-glucan and GAG. Regulation of the biosynthesis of the two polysaccharides could be a potential way of controlling formation of hyphal pellets.

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Influence of α-1,3-glucan synthase gene agsE on protoplast formation for transformation of Aspergillus luchuensis

Cited by 16 publications

References 18 publications

Quantitative Monitoring of Mycelial Growth of Aspergillus fumigatus in Liquid Culture by Optical Density

Quantitative Monitoring of Mycelial Growth of Aspergillus fumigatus in Liquid Culture by Optical Density

A Glycosylphosphatidylinositol-Anchored α-Amylase Encoded by amyD Contributes to a Decrease in the Molecular Mass of Cell Wall α-1,3-Glucan in Aspergillus nidulans

The mechanisms of hyphal pellet formation mediated by polysaccharides, α-1,3-glucan and galactosaminogalactan, in Aspergillus species

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