Influence on proliferation and adhesion of human gingival fibroblasts from different titanium surface decontamination treatments: An in vitro study

Cao, Jie; Wang, Tong; Pu, Yinfei; Tang, Ziyang; Meng, Huaqing

doi:10.1016/j.archoralbio.2017.12.013

Cited by 27 publications

(26 citation statements)

References 34 publications

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“…Ideally, these decontamination methods should not only remove effectively the attached biofilm and calculus, but also avoid any significant deleterious changes at the implant surface (Louropoulou, Slot, Slot, & Weijden, , ), since changes in the implant surface may affect the subsequent proliferation of soft and hard tissue cells, as well as the recolonization by bacterial biofilms (Cao, Wang, Wang, Pu, Tang, & Meng, ). It is well known that bone lining cells and bacteria have a preference for moderately rough surfaces (Jayaraman, Meyer, Meyer, Buhner, Joos, & Wiesmann, ; Sammons, Lumbikanonda, Lumbikanonda, Gross, & Cantzler, ; Teughels, Assche, Sliepen, & Quirynen, ), while on the contrary, fibroblasts and epithelial cells appear to attach better to smooth surfaces (Kononen, Hormia, Hormia, Kivilahti, Hautaniemi, & Thesleff, ; Mustafa, Silva Lopez, Silva Lopez, Hultenby, Wennerberg, & Arvidson, ).…”

Section: Introductionmentioning

confidence: 99%

The effect of five mechanical instrumentation protocols on implant surface topography and roughness: A scanning electron microscope and confocal laser scanning microscope analysis

Kook

Paeng

Jung

et al. 2019

Clinical Oral Implants Res

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Objective:To evaluate in vitro the changes in implant surface topography and roughness of commercial implants after instrumentation with five decontamination protocols.Material and methods: Seventy-two titanium implants with a sandblasted and acidetched (SLA) surface were placed 5 mm supra-crestally. Five groups of twelve implants were instrumented with the following protocols: a metal scaler tip (SCAL), a thermoplastic scaler tip (PEEK), a round titanium brush (RBRU), a tufted brush with titanium bristles (TNBRU), and a glycine-based air-powder abrasive (GLYC). A sixth group with untreated implants was used as control. Scanning electron microscope and confocal laser scanning microscope were utilized to evaluate the changes in the implant surfaces. Results:The SCAL caused pronounced macroscopic alterations and damage of the implant surface, the PEEK left remnants of the plastic tip in the implant surface, and both titanium brush groups flattened the thread profile, while minimal alterations were observed in the GLYC. When compared to the control group, the roughness parameters (Sa) in the buccal aspect increased in the thread area of SCAL, and a minor reduction was observed in the PEEK while in the other groups, these values remained unchanged. In the valley areas, however the RBRU, TNBRU, and GLYC experienced a significant reduction (smoothening) indicating different accessibility of the decontamination protocols to the thread and valley. Similarly, the buccal aspects had more pronounced changes than those in the palatal aspect. Conclusion:Within the limitations of this in vitro investigation, the tested protocols induced different macroscopic alterations and surface roughness changes that varied in the thread and valley area. K E Y W O R D Sconfocal microscope, dental implants, peri-implantitis, scanning electron microscope, surface decontamination, surface macrotopography, titanium | 579 CHA et Al.

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Section: Introductionmentioning

confidence: 99%

The effect of five mechanical instrumentation protocols on implant surface topography and roughness: A scanning electron microscope and confocal laser scanning microscope analysis

Kook

Paeng

Jung

et al. 2019

Clinical Oral Implants Res

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“…In the present study, human gingival fibroblasts were used in a similar strategy as per other reports in the literature [10, 15, 22–25]. In spite of in vivo studies representing the ideal environment to investigate tissue responses, in vitro studies facilitate the investigation of a specific and isolated factor of the tissue response [26].…”

Section: Discussionmentioning

confidence: 99%

“…Cao et al [25] with the aim of investigating the effects of different decontamination treatments on the microstructure of titanium surfaces as well as the proliferation and adhesion of human gingival fibroblasts showed that proliferation and adhesive strength were higher in the machined surfaces than on treated surfaces. These results led the group to conclude that proliferation and adhesion increases as surface roughness decreases.…”

Section: Discussionmentioning

confidence: 99%

Viability and collagen secretion by fibroblasts on titanium surfaces with different acid-etching protocols

Souza

Manfro²,

Joly

et al. 2019

Int J Implant Dent

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BackgroundFrom the consolidation of surface treatments of dental implants and knowledge on the cellular mechanisms of osseointegration, studies have highlighted the importance of a connective tissue seal against the implant to prevent contamination from the oral environment and consequent biofilm formation.ObjectiveThis in vitro study aimed to evaluate whether different titanium surface treatments using acid solutions promoted an increase in collagen secretion, proliferation, and viability of fibroblasts.Material and methodsCommercially pure grade-4 titanium disks (6 × 2 mm) were treated with different acid solutions (hydrochloric, nitric, and sulfuric) for 20 and 60 min, respectively, obtaining mean surface roughness of 0.1 to 0.15 μm and 0.5 to 0.7 μm. Human fibroblasts were seeded onto different surfaces and assessed after 24 h, 48 h, and 72 h for cell proliferation and viability using Trypan blue staining and MTT, respectively, as well as the secretion of type I collagen on to such surfaces using ELISA. Machined titanium surfaces were used as controls. Data were statistically analyzed using one-way ANOVA and Fisher's LSD test for multiple comparisons, adopting a significance level of 5%.ResultsNo significant difference was observed in cell proliferation for the different surfaces analyzed. Cell viability was significantly lower on the machined surface, after 48 h, when compared to the groups treated with acid for 20 or 60 min, which did not differ from each other. The expression of type I collagen was lowest on the acid-treated surfaces.ConclusionThe results showed that the acid treatment proposed did not promote fibroblast proliferation and viability nor favor type I collagen synthesis.

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“…Human gingival fibroblast cells (hGFCs) were obtained from the residual gingiva of wisdom teeth extracted from patients in the clinic . Informed consent was acquired from patients before wisdom tooth extraction surgery.…”

Section: Methodsmentioning

confidence: 99%

“…Human gingival fibroblast cells (hGFCs) were obtained from the residual gingiva of wisdom teeth extracted from patients in the clinic. 41 Informed consent was acquired from patients before wisdom tooth extraction surgery. The gingival tissue was cut into 1 mm diameter pieces and cultured in low glucose Dulbecco's-modified Eagle's medium (SH30021; HyClone, Logan, UT) with fetal bovine serum and penicillin-streptomycin in a humidified incubator with 5% CO 2 at 37 C. Then, the cells were subcultured at a ratio of 1:2 when their confluence reached approximately 80%.…”

Section: Cytotoxicity Test 281 | Culture Of Rat Bone Marrow Strommentioning

confidence: 99%

A thermosensitive chitosan‐based hydrogel for sealing and lubricating purposes in dental implant system

Cao

Cai

Chen

et al. 2019

Clin Implant Dent Rel Res

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Background Mechanical and biological complications associated with implant systems happen frequently in the clinic. Purpose To develop a chitosan (CS)‐based thermosensitive hydrogel for sealing and lubricating purposes in dental implant system. Materials and Methods In this study, a thermosensitive hydrogel made up of CS, β‐glycerophosphate pentahydrate (β‐GP), and povidone‐iodine (PVP‐I), called CS/β‐GP/PVP‐I thermosensitive hydrogel, was fabricated. Three experimental groups with different volume ratios of CS to β‐GP were prepared, namely 16/4, 13/7, and 10/10 groups. The surface topography of the different groups and their physicochemical characteristics were examined by SEM, FTIR, and X‐ray diffraction analysis. The cytotoxicity of the hydrogel was examined by CCK‐8 test. In vitro antibacterial efficiency was analyzed by the spread plate method. Sealing ability was detected by incubating two‐piece implants in Escherichia coli suspension. Lubricating ability of the hydrogel was evaluated by the removal torque test with a calibrated digital torque meter. Results The CS/β‐GP/PVP‐I thermosensitive hydrogel was fabricated and showed a highly porous structure under SEM. An in vitro cytotoxicity test demonstrated that 13/7 group displayed no cytotoxicity. Furthermore, all three groups showed obviously antibacterial effects. In the sealing ability test, 16/4 group showed the best sealing ability. The removal torque of 16/4 group and 13/7 group was significantly greater than control group. Conclusions Based on our findings, it could be concluded that the thermosensitive and antibacterial CS/β‐GP/PVP‐I hydrogel with sealing and lubricating ability was successfully prepared. The hydrogel had better sealing and lubricating effects when the volume ratio of CS to β‐GP was 16/4.

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Influence on proliferation and adhesion of human gingival fibroblasts from different titanium surface decontamination treatments: An in vitro study

Cited by 27 publications

References 34 publications

The effect of five mechanical instrumentation protocols on implant surface topography and roughness: A scanning electron microscope and confocal laser scanning microscope analysis

The effect of five mechanical instrumentation protocols on implant surface topography and roughness: A scanning electron microscope and confocal laser scanning microscope analysis

Viability and collagen secretion by fibroblasts on titanium surfaces with different acid-etching protocols

A thermosensitive chitosan‐based hydrogel for sealing and lubricating purposes in dental implant system

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