Influences of calcium/phosphorus ratio on supplemental microbial phytase efficiency for Nile tilapia (Oreochromis niloticus)

Hassaan, Mohamed S.; Soltan, Magdy A.; Agouz, H. M.; Badr, Anas

doi:10.1016/j.ejar.2013.09.001

Cited by 45 publications

(30 citation statements)

References 39 publications

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“…The largest increases in body weight and feed utilization were observed in groups that received diet with a mixture of malic acid and the probiotic; however, the addition of the acid or B. subtilis alone also significantly improved the growth performance of the fish. That supplementing diets with organic acid or salts stimulates growth has been reported previously in Nile tilapia (Hassaan, Soltan, Agouz, & Badr, ; Hassaan, Soltan, & Ghonemy, ; Hassaan, Wafa, Soltan, Goda, & Mogheth, ; Meshrf, ; Reda, Mahmoud, Selim, & El‐Araby, ) and rainbow trout, Oncorhynchus mykiss (De Wet, ). Increased growth in fish fed diets supplemented with a probiotic has also been observed in trout (Gatesoupe, ), rohu , Labeo rohita (Bairagi, Sarkar Ghosh, Sen, & Ray, ), grass carp, Ctenopharyngodon idella (Wang, ) and tilapia (Aly, Ahmed, Ghareeb, & Mohamed, ; Hassaan, Soltan et al., ; Hassaan, Wafa et al., ).…”

Section: Discussionsupporting

confidence: 64%

Combined effects of dietary malic acid andBacillus subtilison growth, gut microbiota and blood parameters of Nile tilapia (Oreochromis niloticus)

et al. 2017

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The study investigated effect of dietary supplementation with malic acid, Bacillus subtilis and a mixture of the two (3 × 2 factorial trial) on Nile tilapia (Oreochromis niloticus) health. Treatment groups (T1-T6) were fed diets containing three levels of malic acid bacterial count in the gut and faeces was detected in fish from group T6. The values of haematocrit, haemoglobin, red blood cells, white blood cells, total protein, albumin and globulin were significantly higher (p < .05) in fish from groups T4 and T6 (diets supplemented with malic acid and B. subtilis). As compared to the control fed fish, te mixture of these substances are promising as immune enhancher in aquacultured fish. K E Y W O R D SBacillus subtilis, blood parameters, growth, gut microbiota, Nile tilapia, organic acid | INTRODUCTIONMicrobial balance and proper pH in the digestive tract eliminate pathogenic microorganisms. This is necessary to maintain fish health in a satisfactory state and to achieve expected production levels.Over the last decade, research has focused on the application of probiotics and prebiotics to replace antibiotic growth promoters in the fish farming industry (Irianto & Austin, 2002;Yanbo & Zirong, 2006) and to enhance the growth and health of the host (Gatesoupe, 1999;Kesarcodi-Watson, Kaspar, Lategan, & Gibson, 2008).Most commercial probiotics currently being tested in aquaculture are lactic acid bacteria (e.g., Lactobacillus), the genus Bacillus, photosynthetic bacteria (e.g., Rhodobacter sphaeroides) and the yeasts foods as a flavour enhancer and for pH (1% malic acid solution is about 2.3) and microbial control, because it inhibits the growth of pathogenic bacteria and pathogenic fungi (Ricke, 2003). An exceptionally valuable nutritional property of this acid is that after oral administration, it stimulates the secretion of gastric juices and increases peristalsis. Small doses of malic acid have prebiotic properties, stimulating the development of non-pathogenic, acidophilic and saprophytic bacteria whereas at higher doses it can exhibit bacteriostatic and bactericidal properties (Salou, Leroy, Goma, & Pareilleux, 1991).An extensive review of the literature on this topic revealed that there are no reports on the in vivo kinetics of probiotic bacterial growth in the presence of organic acids. In in vitro studies, Lactobacillus plantarum exhibited better growth in the presence of malic acid as compared to the control group (Passos, Fleming, Hassan, & McFeeters, 2003).Therefore, in the authors' opinion, the current study is innovative and facilitates a better understanding of the interactions occurring among probiotics and malic acid in the gastrointestinal tract of fish. In summation, the aim of the current investigation was to assess the dietary inclusion of malic acid, Bacillus subtilis or a mix of the two on the growth performance, feed utilization, gut microbiota and haematological and biochemical blood parameters of Nile tilapia (Oreochromis niloticus;Linnaeus, 1758). After carp, the tilapia species c...

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Section: Discussionsupporting

confidence: 64%

Combined effects of dietary malic acid andBacillus subtilison growth, gut microbiota and blood parameters of Nile tilapia (Oreochromis niloticus)

et al. 2017

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“…A definite correlation between serum calcium and phosphorus ratio was recorded in fish fed with different diets [42,43]. The result of the present investigation also indicated a positive correlation between the Ca/P ratio and dietary protein level and quality source.…”

Section: Citationsupporting

confidence: 61%

Evaluating the Nutritional Condition of an Indian Climbing Perch, Anabas Testudineus Fingerlings by the RNA/DNA, Ca/P Ratio and Protein Bio-Synthesis in Liver and Muscle

Patra¹,

Patra²,

Bhattacharya³

2017

Fish Aquac J

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Early fingerlings of Anabas testudineus were nurtured in the laboratory for six weeks under altered nutritional status (different dietary protein level and source) and feeding regimes (ad libitum, 'limited', and 'starvation' intervals). Their RNA/DNA, Ca/P ratios and protein biosynthesis were determined with highly sensitive fluorescence technique, spectrophotometer and liquid scintillation system respectively. Well-fed early fingerlings (4-6 weeks age group) having higher RNA/DNA (2.11), Ca/P (1.82) ratio and protein biosynthesis rates both in muscle and liver have significantly differs from fingerlings with 'limited' food supply and 'starvation' intervals. Rate of protein biosynthesis >244.33 & 603.24 in muscle & liver respectively (count per minute mg protein -1 ) when compared with well-fed fingerlings and reared under 'limited' food supply and 'starvation' intervals. At 'starvation' interval no significant differences were recorded due to different diet treatment. However, fingerlings showed higher mortalities, decreased growth, RNA/DNA, Ca/P ratio and protein biosynthesis rate that may be caused by malnutrition. Fingerlings (early) sampled after 42 days in nearby areas during late monsoon (September) showed RNA/DNA, Ca/P ratio and protein biosynthesis rate were surprisingly low almost as low as 'starved' fingerlings, when judged on the basis of mean body weight as compared to well fed fish. It was also observed under microscope that some of these fingerlings were seriously affected by an intestinal disease and this may be due to the ingestion of unsuitable copepods. The low RNA/DNA, Ca/P ratio and protein biosynthesis rate in field samples and 'starved' fish are, therefore, interpreted as a sub-lethal result of this disease and malnutrition respectively.

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“…Producers have long used synthetic phytase as an additive to soy‐based feeds for mono‐ and agastric animals to compensate for the natural lack of this enzyme and improve the feed's efficiency. However, this method is both expensive and less efficient than natural phytase activity, as it relies heavily on various factors including temperature, pH, and mineral concentration (Brejnholt et al, 2011; Hassaan et al, 2013).…”

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Impact of mips1, lpa1, and lpa2 Alleles for Low Phytic Acid Content on Agronomic, Seed Quality, and Seed Composition Traits of Soybean

et al. 2017

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Soybean [Glycine max (L.) Merr] is an important agronomic crop around the world, used largely for animal feed. However, ∼75% of the phosphorus (P) in soybean grain is in the form of phytic acid (PA) or phytate, the cation salt form of PA, which cannot be digested by monogastric and agastric animals including swine, poultry, and aquacultural animals, leading to decreased field efficiency and environmental detriment due to P runoff. Soybean lines have been developed with a reduced PA content using mutant alleles of three genes involved in the PA pathway: either the combination of lpa1 and lpa2 or mips1. However, the relationship among these alleles was unknown. This study was conducted to explore the impact of these three mutant alleles on soybean agronomic, seed quality, and seed composition traits. A population consisting of 30 recombinant inbred lines developed from a cross between V03‐5901 (mips1) × 04‐05N32 (lpa1/lpa2) was planted along with the parents at two locations in Virginia in 2014 and 2015. The results showed that (i) an additive relationship existed among the three different mutant alleles, resulting in lower PA content as more low‐PA (LPA) mutant alleles were added; (ii) the lines with lpa1 allele had the highest field emergence, and thus lpa1 may be a good trait with which to create a commercially viable LPA soybean cultivar; and (iii) the interaction between the mips1 and lpa2 alleles resulted in raffinose content significantly lower than with either allele on its own. The findings of this study provided breeders with references for developing LPA soybean cultivars combined with other desirable traits.

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Influences of calcium/phosphorus ratio on supplemental microbial phytase efficiency for Nile tilapia (Oreochromis niloticus)

Cited by 45 publications

References 39 publications

Combined effects of dietary malic acid andBacillus subtilison growth, gut microbiota and blood parameters of Nile tilapia (Oreochromis niloticus)

Combined effects of dietary malic acid andBacillus subtilison growth, gut microbiota and blood parameters of Nile tilapia (Oreochromis niloticus)

Evaluating the Nutritional Condition of an Indian Climbing Perch, Anabas Testudineus Fingerlings by the RNA/DNA, Ca/P Ratio and Protein Bio-Synthesis in Liver and Muscle

Impact of mips1, lpa1, and lpa2 Alleles for Low Phytic Acid Content on Agronomic, Seed Quality, and Seed Composition Traits of Soybean

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