Influences of dietary n−3 long‐chain PUFA on body concentrations of 20∶5n−3, 22∶5n−3, and 22∶6n−3 in the larvae of a marine teleost fish from Australian waters, the striped trumpeter (Latris lineata)

Abstract

We determined the effect of dietary long-chain (> or = C20) PUFA (LC-PUFA), 20:5n-3 and 22:6n-3, on larval striped trumpeter (Latris lineata) biochemistry through early development and during live feeding with rotifers (Brachionus plicatilis). Rotifers were enriched using seven experimental emulsions formulated with increasing concentrations of n-3 LC-PUFA, mainly 20:5n-3 and 22:6n-3. Enriched rotifer n-3 LC-PUFA concentrations ranged from 10-30 mg/g dry matter. Enriched rotifers were fed to striped trumpeter larvae from 5 to 18 d post-hatch (dph) in a short-term experiment to minimize gross deficiency symptoms such as poor survival that could confound results. No relationships were observed between larval growth or survival with dietary n-3 LC-PUFA at 18 dph. The larval FA profiles generally reflected those of the rotifer diet, and significant positive regressions were observed between most dietary and larval FA at 10, 14, and 18 dph. The major exception observed was an inverse relationship between dietary and larval 22:5n-3. The presence of 22:5n-3 in elevated amounts when dietary 22:6n-3 was depressed suggests that elongation of 20:5n-3 may be occurring in an attempt to raise body concentrations of 22:6n-3. We hypothesize that accumulation of 22:5n-3 might be an early indicator of 22:6n-3 deficiency in larval fish that precedes a reduction in growth or survival. A possible role of 22:5n-3 as a biochemical surrogate for 22:6n-3 is discussed.

Effects of dietary n‐3 PUFA supplements on composition of n‐3 PUFA and expression of fatty acid elongase 5 (AJELOVL5) in sea cucumber, Apostichopus japonicus

“…The ALA level was high in the intestine of sea cucumbers fed diet ALA 12.71 , followed by those fed diets ALA 7.46 and ALA 0 . The content of EPA and DHA in tissues increased with increasing dietary EPA and DHA, which is in consistent with the PUFA level reported in the muscle of C. striata and Latris lineate larvae before 18 days posthatching (Bransden et al, ; Jaya‐Ram, et al, ).…”

Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae

“…The dietary fatty acid data is limited by the lack of replication, and needs to be interpreted with caution. Nonetheless, there were strong relationships among dietary fatty acids and fatty acid composition of larvae in both experiments as has been demonstrated for striped trumpeter previously (Bransden et al, 2004) and other species (Mourente et al, 1993;Rodriguez et al, 1994;Koven et al, 2001). Rotifers enriched with AlgaMac, under comparable conditions in our laboratory, have been reported to have up to 20% greater total fatty acid but lower proportions of essential LC-PUFA.…”

“…An aliquot of total lipid was also transesterified with a methanol/chloroform/hydrochloric acid (10 : 1 : 1, by vol) solution to form fatty acid methyl esters (FAME). The extraction and methylation procedures have been validated for a range of small samples including microheterotrophs (Lewis et al, 2000), striped trumpeter larvae and rotifers (Bransden et al, 2004;unpublished data). FAME were extracted, concentrated and an internal injection standard (methyl tricosanoate) added, the latter for Experiment 2 animals only.…”

“…FAME were extracted, concentrated and an internal injection standard (methyl tricosanoate) added, the latter for Experiment 2 animals only. FAME were analysed by gas chromatography (GC) according to Bransden et al (2004).…”

Weaning Strategies for Striped Trumpeter, Latris lineata, Postlarvae Culture

“…Despite recent advances, hatchery‐produced juveniles have exhibited a high incidence of malformations (Cobcroft et al 2001; Trotter et al 2001; Battaglene and Cobcroft 2007). Research has identified the required content of long‐chain fatty acids and selected vitamins in live feeds (Bransden et al 2004a, 2004b, 2005a, 2005b; Brown et al 2005a,b). Historically, striped trumpeter postlarvae were reared using enriched Artemia until 100 d posthatch (dph).…”