Influences of different stress media and high light intensities on accumulation of astaxanthin in the green alga Haematococcus pluvialis

Abstract

Haematococcus pluvialis Flotow is used in the aquaculture, pharmaceutical and cosmetic industries. The aim of this study was to compare the effect of various stress media and high light intensities on astaxanthin accumulation. The experimental design was achieved by four different stress media and two different light intensities for 14 days of induction period. The astaxanthin concentrations of 29.62 mg g(-1) and 30.07 mg g(-1) were obtained in distilled water with CO(2) and N-free medium, respectively, with no significant difference between them at 546 micromol photons m(-2)s(-1). Because of the morphological changes of H. pluvialis, microscopic observation was considered during the induction period to facilitate the selection of stress medium. It was clear that the rate of astaxanthin accumulation was much faster in distilled water with the addition of CO(2). The main point is that, this medium is more economical than others, especially for the large-scale productions.

Carotenogênese em células de Haematococcus pluvialis induzidas pelos estresses luminoso e nutricional

“…A microalga foi cultivada em meio WC (Guillard & Lorenzen, 1972), com pH 7,0 e a seguinte composição final: NaNO 3 (1,0x10…”

“…Diversos estudos vêm sendo conduzidos para aumentar o rendimento da produção de astaxantina durante o cultivo de H. pluvialis (Sarada et al, 2002;Cifuentes et al, 2003;Domínguez-Bocanegra et al, 2004;Imamoglu et al, 2009). Grande parte dos resultados mostra que a adição de mais de um fator estressante à célula, até devida proporção, aumenta a produtividade final deste carotenoide na biomassa (Fabregas et al, 2003;Saha et al, 2013).…”

“…Entre as formas mais funcionais de estresse celular, destacam-se: a privação de nutrientes específicos, como nitrogênio ou fósforo (Fabregas et al, 2003;Imamoglu et al 2009); a adição de NaCl (Kobayashi et al, 1997); o estresse oxidativo, promovido por meio da adição de ferro (Kobayashi et al, 1993); o aumento da relação C/N disponível para a célula (Kang et al, 2007); e o aumento da intensidade luminosa (Wang et al, 2003;Torzillo et al, 2005).…”

“…Esta relação positiva entre o incremento da iluminação e o desempenho da carotenogênese foi observada em células submetidas a um aumento de quatro (Kang et al, 2007) e de seis a sete vezes o valor inicial da intensidade luminosa (Imamoglu et al, 2009).…”

Biotechnological potential of an isolated and a known green microalgal strain and their cultivation in biofilm based reactors

“…N limitation connected to high light is one of the most efficient factors to trigger high astaxanthin content as it was described by several authors reporting astaxanthin contents of 2-5% both in suspension and attached cultivation [59,82,85,112,[121][122][123].…”