Influences of DMP on the Fertilization Process and Subsequent Embryogenesis of Abalone (Haliotis diversicolor supertexta) by Gametes Exposure

Zhou, Jin; Zhu, Xiaoshan; Cai, Zhonghua

doi:10.1371/journal.pone.0025951

Cited by 9 publications

(2 citation statements)

References 54 publications

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“…Most of the studies focus on marine species, and the molecular analysis in freshwater species is basically absent. Furthermore, in gastropods, the studies are centered on the marine representative Haliotis diversicolor [16][17][18] . The freshwater snail Physella acuta (Draparnaud, 1805), also known as Physa acuta, belongs to the Physidae family.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Impact of Three Phthalates on the Freshwater Gastropod Physella Acuta at the Transcriptional Level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Preprint

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Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the phthalates' effects have been extensively studied on vertebrates, there is a gap in knowledge on their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of thirty genes in one-week exposed animals at 0.1, 10, and 1000 µg/L. The genes cover the DNA repairing mechanism, detoxification mechanisms, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not cause alteration of the mRNA levels, BBP modulates almost all the genes tested. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on this gastropod.

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Section: Introductionmentioning

confidence: 99%

Analysis of the Impact of Three Phthalates on the Freshwater Gastropod Physella Acuta at the Transcriptional Level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Preprint

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show abstract

“…The few studies have focused on marine species, and the molecular analysis in freshwater species is basically absent. In gastropods, the studies to date have centered on the marine representative Haliotis diversicolor 17 – 19 , showing that phthalates produce oxidative stress and alterations in lipid and energy metabolism. The freshwater snail Physella acuta (Draparnaud, 1805), also known as Physa acuta , belongs to the Physidae family.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Sci Rep

View full text Add to dashboard Cite

Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the effects of phthalates on vertebrates have been extensively studied, there is a knowledge gap regarding their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of 30 genes in animals exposed for 1 week to 0.1, 10, and 1000 μg/L of each phthalate. The genes cover DNA repair, detoxification, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not alter the mRNA levels, BBP modulated almost all the analyzed genes. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on the gastropod P. acuta.

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Screening of phthalate esters in 47 branded perfumes

Al‐Saleh

Elkhatib

2015

Environ Sci Pollut Res

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In the last few years, the use of phthalates in perfumes has gained attention because these chemicals are sometimes added intentionally as a solvent and a fixative. Five phthalate esters, dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), and diethyl hexyl phthalate (DEHP), were measured in 47 branded perfumes using headspace solid phase microextraction (SPME) followed by gas chromatography-mass spectrometry (GC-MS). The results revealed considerable amounts of phthalate in all 47 brands with detection frequencies > limit of quantitation in the following order: DEP (47/47) > DMP (47/47) > BBP (47/47) > DEHP (46/47) > DBP (23/45). Of the 47 brands, 68.1, 72.3, 85.1, 36.2, and 6.7 % had DEP, DMP, BBP, DEHP, and DBP levels, respectively, above their reported threshold limits. Of these phthalates, DEP was found to have the highest mean value (1621.625 ppm) and a maximum of 23,649.247 ppm. The use of DEP in the perfume industry is not restricted because it does not pose any known health risks for humans. DMP had the second highest level detected in the perfumes, with a mean value of 30.202 ppm and a maximum of 405.235 ppm. Although DMP may have some uses in cosmetics, it is not as commonly used as DEP, and again, there are no restrictions on its use. The levels of BBP were also high, with a mean value of 8.446 ppm and a maximum of 186.770 ppm. Although the EU banned the use of BBP in cosmetics, 27 of the tested perfumes had BBP levels above the threshold limit of 0.1 ppm. The mean value of DEHP found in this study was 5.962 ppm, and a maximum was 147.536 ppm. In spite of its prohibition by the EU, 7/28 perfumes manufactured in European countries had DEHP levels above the threshold limit of 1 ppm. The DBP levels were generally low, with a mean value of 0.0305 ppm and a maximum value of 0.594 ppm. The EU banned the use of DBP in cosmetics; however, we found three brands that were above the threshold limit of 0.1 ppm, and all were manufactured in European countries. The results of this study are alarming and definitely need to be brought to the attention of the public and health regulators. Although some phthalate compounds are still used in cosmetics, many scientists and environmental activists have argued that phthalates are endocrine-disrupting chemicals that have not been yet proven to be safe for any use, including cosmetics. Phthalates may also have different degrees of estrogenic modes of action. Furthermore, we should not dismiss the widespread use of phthalates in everyday products and exposure to these chemicals from sources such as food, medications, and other personal care products.

show abstract

Influences of DMP on the Fertilization Process and Subsequent Embryogenesis of Abalone (Haliotis diversicolor supertexta) by Gametes Exposure

Cited by 9 publications

References 54 publications

Analysis of the Impact of Three Phthalates on the Freshwater Gastropod Physella Acuta at the Transcriptional Level

Analysis of the Impact of Three Phthalates on the Freshwater Gastropod Physella Acuta at the Transcriptional Level

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Screening of phthalate esters in 47 branded perfumes

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