Influences of hydrozoan colonization on proteomic profiles of the brown alga <i>Saccharina japonica</i>

Getachew, Paulos; Nam, Bo‐Hye; Cho, Ji Young; Hong, Yong‐Ki

doi:10.1515/bot-2015-0103

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…The development of proteomics allows comparative studies of protein expression changes in response to changes in the environment (Tee et al, 2015; Getachew et al, 2016). Proteomic approaches are important tools to elucidate the mechanisms underlying the ability of seaweed species to tolerate a wide range of abiotic (Contreras-Porcia & López-Cristoffanini, 2012) and biotic conditions (Getachew et al, 2014).…”

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confidence: 99%

Environmental modulation of the proteomic profiles from closely phylogenetically related populations of the red seaweed Plocamium brasiliense

Calegário

Freitas

Santos

et al. 2019

PeerJ

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The genus Plocamium encompasses seaweeds that are widely distributed throughout the world’s oceans, with Plocamium brasiliense found along the tropical and subtropical coasts of the Western Atlantic. This wide distribution can lead to structured populations due to environmental differences (e.g., light levels or temperature), restricted gene flow, and the presence of cryptic species. Abiotic variation can also affect gene expression, which consequently leads to differences in the seaweeds protein profile. This study aimed to analyze the genetic and proteomic profiles of P. brasiliense sampled in two geographically distinct sites on the coastline of Rio de Janeiro state, Brazil: Arraial do Cabo (P1) and Búzios (P2). The genetic profiles of macroalgal specimens from these two sites were indistinguishable as assessed by the markers UPA/23S, rbcL, and COI-5P; however, the protein profiles varied significantly between populations from the two sites. At both sites the ribulose-1,5-biphosphate carboxylase/oxygenase was the most abundant protein found in P. brasiliense specimens. The number of phycobiliproteins differed between both sites with the highest numbers being found at P1, possibly due to water depth. The differences in proteomic profiles of the two nearly identical populations of P. brasiliense suggest that environmental parameters such as light availability and desiccation might induce distinct protein expression, probably as a result of the phenotypic plasticity within this population of seaweed.

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Section: Introductionmentioning

confidence: 99%

Environmental modulation of the proteomic profiles from closely phylogenetically related populations of the red seaweed Plocamium brasiliense

Calegário

Freitas

Santos

et al. 2019

PeerJ

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Identification of early biomarkers in proteomic profiles of the phaeophyteSaccharina japonicaproximal to and beneath the front of bryozoan colonies

2018

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The sessile bryozoanMembranipora membranaceafrequently colonizes the phaeophyteSaccharina japonica.Identifying early colonization markers using proteomics could assist in the early detection of epiphytic contamination. Different sections of thallus tissue proximal to the bryozoan (i.e. the 1-cm zone beyond the boundary of the colony) and tissue from the colony-front (i.e. the narrow zone under the newly formed front of the colony after removing the bryozoans) were separated. From the proteomic profiles ofS. japonica, we detected 151 protein spots (99 up-, 50 down-, and 2 similarly regulated) from proximal tissues and 151 spots (69 up-, 75 down-, and 7 same-regulated) from colony-front tissues. Hundred and ten spots were detected from distal healthy thallus tissue, used as a control. The protein SSP15 was specifically up-regulated in the proximal tissues by ca. 1395-fold, while it exhibited little expression at the colony-front and in distal healthy tissues. ATPases were markedly up-regulated in both the proximal and colony-front tissues by 3198- and 2475-fold, respectively. Rpl1P and SRSF proteins were specifically up-regulated only in colony-front tissues by 5724- and 273-fold, respectively. Therefore, these proteins may be used as specific biomarkers for the early detection of bryozoan colonization on each tissue type of the seaweed.

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Comparison of proteomic profiles of the phaeophyte Saccharina japonica thalli proximal to and beneath the front of epiphytic hydrozoan colonies against healthy tissue

2019

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The stoloniferous hydrozoan Obelia geniculata commonly colonizes macroalgae such as Saccharina japonica. Each Obelia colony consists of thread-like hydrorhizae attached to the seaweed thallus. The early signaling proteins of epiphytic contamination can be identified using proteomics. To isolate these early signals, parts of the thallus proximal to the hydrozoans were separated from beneath the colony front and from healthy tissue. From the proteomic profiles of S. japonica, we detected 110 protein spots from tissue proximal to hydrozoan colonies (56 increased, 53 decreased, and 1 unchanged in expression relative to healthy tissue) and 133 spots from tissue at the colony front (67 increased, 60 decreased, and 6 unchanged in expression). Of the proteins with increased and decreased expression, SIPA1L1 and actin increased strongly only in the proximal tissues, while NEK2 kinase decreased. CIPK20 and SIPA1L2 increased markedly in both the colony-front and proximal tissues, while CaMK2N2 and GK25369 decreased in both tissues. ATPase β, ADA, kinesin, and HECT domain proteins increased only in the colony-front tissues. Among them, SIPA1L2 increased strongly in both the thallus tissues proximal to the hydrozoans and those beneath the colony front, but was not expressed in response to bryozoan infection.

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Influences of hydrozoan colonization on proteomic profiles of the brown alga Saccharina japonica

Cited by 3 publications

References 32 publications

Environmental modulation of the proteomic profiles from closely phylogenetically related populations of the red seaweed Plocamium brasiliense

Environmental modulation of the proteomic profiles from closely phylogenetically related populations of the red seaweed Plocamium brasiliense

Identification of early biomarkers in proteomic profiles of the phaeophyteSaccharina japonicaproximal to and beneath the front of bryozoan colonies

Comparison of proteomic profiles of the phaeophyte Saccharina japonica thalli proximal to and beneath the front of epiphytic hydrozoan colonies against healthy tissue

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