Influences of nasal lavage collection-, processing- and storage methods on inflammatory markers — Evaluation of a method for non-invasive sampling of epithelial lining fluid in cystic fibrosis and other respiratory diseases

Hentschel, Julia; Müller, Ulrike; Doht, Franziska; Fischer, Nele; Böer, Klas; Sonnemann, Jürgen; Hipler, C; Hünniger, Kerstin; Kurzai, Oliver; Markert, Udo R.; Mainz, Jochen G.

doi:10.1016/j.jim.2013.12.003

Cited by 47 publications

(50 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was performed in accordance with the standard diagnostic procedure for nasal lavage [18]; head in a slightly reclined position and the soft palate occluded. A protease inhibitor (Protease Inhibitor Mix G, Serva, Germany) was added to the native NL and stored at − 80°C.…”

Section: Nasal Lavage (Nl)mentioning

confidence: 99%

Sino nasal inhalation of isotonic versus hypertonic saline (6.0%) in CF patients with chronic rhinosinusitis — Results of a multicenter, prospective, randomized, double-blind, controlled trial

Mainz

Schumacher

Schädlich

et al. 2016

Journal of Cystic Fibrosis

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Nasal Lavage (Nl)mentioning

confidence: 99%

Sino nasal inhalation of isotonic versus hypertonic saline (6.0%) in CF patients with chronic rhinosinusitis — Results of a multicenter, prospective, randomized, double-blind, controlled trial

Mainz

Schumacher

Schädlich

et al. 2016

Journal of Cystic Fibrosis

Self Cite

View full text Add to dashboard Cite

show abstract

“…One way to measure changes are by using nasal lavage, which is a noninvasive sample technique that also demonstrates the nasal mucosa in a representative manner. The fluid contains excretions from goblets cells and seromucous glands, including epithelial cells and immune active cells, and last plasma exudation that is possible to measure [172][173]. Changes in nasal lavage fluid have been shown in studies of exposure to organic acid anhydrides, wood dust and indoor environmental perception, and in disease states such as allergy, cystic fibrosis and asthma [145,[173][174][175][176].…”

Section: Nasal Lavagementioning

confidence: 99%

“…The fluid contains excretions from goblets cells and seromucous glands, including epithelial cells and immune active cells, and last plasma exudation that is possible to measure [172][173]. Changes in nasal lavage fluid have been shown in studies of exposure to organic acid anhydrides, wood dust and indoor environmental perception, and in disease states such as allergy, cystic fibrosis and asthma [145,[173][174][175][176]. One study reports the use of nasal lavage fluid as an alternative to the more invasive bronchoalveolar lavage to monitor early lower airway inflammation in cystic fibrosis [177].…”

Section: Nasal Lavagementioning

confidence: 99%

“…There are different parameters that can influence the results of the nasal lavage. Studies have shown that time of day, duration of solution in the vestibule, temperature of the solution, performing pre-washing or not, number of repeated measures each day and days in a row may all influence the results gained [173,[180][181]. The concentration, volume and presence of various markers may be altered due to these factors and consequently they are significant to consider when performing a study.…”

Section: Nasal Lavagementioning

confidence: 99%

“…The concentration, volume and presence of various markers may be altered due to these factors and consequently they are significant to consider when performing a study. Also, the management of the samples is important to consider since repeated freeze-thaw cycles, proteases and storage temperatures may influence the end result [173,179]. Nevertheless, the intra-individual variation is lower than the inter-individual variation, making it possible to follow a subject over time to study possible alterations [179].…”

Section: Nasal Lavagementioning

confidence: 99%

See 2 more Smart Citations

Upper Airway Mucosal Inflammation : Proteomic Studies after Exposure to Irritants and Microbial Agents

Fornander¹

2015

View full text Add to dashboard Cite

People are, in their daily lives, exposed to a number of airborne foreign compounds that do not normally affect the body. However, depending on the nature of these compounds, dose and duration of exposure, various airway symptoms may arise. Early symptoms are often manifested as upper airway mucosal inflammation which generates changes in protein composition in the airway lining fluid.This thesis aims at identifying, understanding mechanisms and characterizing protein alterations in the upper airway mucosa that can be used as potential new biomarkers for inflammation in the mucosa. The protein composition in the mucosa was studied by sampling of nasal lavage fluid that was further analyzed with a proteomic approach using two-dimensional gel electrophoresis and mass spectrometry. Additionally, by studying factors on site through environmental examination, health questionnaires and biological analyses, we have tried to understand the background to these protein alterations and their impact on health.Respiratory symptoms from the upper airways are common among people who are exposed to irritative and microbial agents. This thesis have focused on personnel in swimming pool facilities exposed to trichloramine, metal industry workers exposed to metalworking fluids, employees working in damp and moldy buildings and infants diagnosed with respiratory syncytial virus infection. The common denominator in these four studies is that the subjects experience upper airway mucosal inflammation, which is manifested as cough, rhinitis, phlegm etc. In the three occupational studies, the symptoms were work related. Notably, a high prevalence of perceived mucosal symptoms was shown despite the relatively low levels of airborne irritants revealed by the environmental examination. Protein profiling verified an ongoing inflammatory response by identification of several proteins that displayed altered levels. Interestingly, innate immune proteins dominated and four protein alterations occurred in most of the studies; SPLUNC1, protein S100A8 and S100A9 and alpha-1-antitrypsin. Similarly, these proteins were also found in nasal fluid from children with virus infection and in addition a truncated form of SPLUNC1 and two other S100 proteins (S100A7-like 2 and S100A16), not previously found in nasal secretion, were identified.Altogether, the results indicate the potential use of a proteomic approach for identifying new biomarkers for the upper respiratory tract at an early stage in the disease process after exposure to irritant and microbial agents. The results indicate an effect on the innate immunity system and the proteins; SPLUNC1, protein S100A8 and S100A9 and alpha-1-antitrypsin are especially promising new biomarkers. Moreover, further studies of these proteins may help us to understand the molecular mechanisms involved in irritant-induced airway inflammation. POPULÄRVETENSKAPLIG SAMMANFATTNING FÖRÄNDRAD PROTEINSAMMANSÄTTNING I DE ÖVRE LUFTVÄGARNA EFTER EXPONERING FÖR SLEMHINNEIRRITERANDE ÄMNENVarje dag utsätts vi för partikla...

show abstract

Effects of a honeybee lactic acid bacterial microbiome on human nasal symptoms, commensals, and biomarkers

Mårtensson

Greiff

Lamei

et al. 2016

Int Forum Allergy Rhinol

View full text Add to dashboard Cite

show abstract

Influences of nasal lavage collection-, processing- and storage methods on inflammatory markers — Evaluation of a method for non-invasive sampling of epithelial lining fluid in cystic fibrosis and other respiratory diseases

Cited by 47 publications

References 45 publications

Sino nasal inhalation of isotonic versus hypertonic saline (6.0%) in CF patients with chronic rhinosinusitis — Results of a multicenter, prospective, randomized, double-blind, controlled trial

Sino nasal inhalation of isotonic versus hypertonic saline (6.0%) in CF patients with chronic rhinosinusitis — Results of a multicenter, prospective, randomized, double-blind, controlled trial

Upper Airway Mucosal Inflammation : Proteomic Studies after Exposure to Irritants and Microbial Agents

Effects of a honeybee lactic acid bacterial microbiome on human nasal symptoms, commensals, and biomarkers

Contact Info

Product

Resources

About