Influences of opioids and nanoparticles on in vitro wound healing models

Wolf, Nina; Küchler, Sarah; Radowski, Michał R.; Blaschke, Tobias; Kramer, K.D.; Weindl, Günther; Kleuser, Burkhard; Haag, Rainer; Schäfer‐Korting, Monika

doi:10.1016/j.ejpb.2009.03.009

Cited by 79 publications

(88 citation statements)

References 71 publications

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“…In ischemic wounds, locally applied opioids hastened wound closure increased granulation tissue and collagen formation, increased epidermal and dermal organization, and enhanced angiogenesis (Poonawala et al, 2005;Gross et al, 2009). The vascular endothelial-derived growth factor receptor Flk1 and nitric oxide were also up-regulated by opioids in rat (Poonawala et al, 2005) and human keratinocytes (Wolf et al, 2009). However, depending on the chronicity of the wound and the stage of the healing process, some groups reported retarding effects in models of excisional wounds (Rook et al, 2008) and corneal abrasions (Sassani et al, 2003).…”

Section: Peripheral Pain Inhibitionmentioning

confidence: 99%

Modulation of Peripheral Sensory Neurons by the Immune System: Implications for Pain Therapy

2011

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Section: Peripheral Pain Inhibitionmentioning

confidence: 99%

Modulation of Peripheral Sensory Neurons by the Immune System: Implications for Pain Therapy

2011

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“…The method established titanium oxide nanoparticles to be effective against tumor cells [18]. Lipid nanoparticles of opioids enhanced inhibition capacities of opioids by twofold in comparison with CMS nanotransporters when evaluated in HaCaT cells [19].…”

Section: Wound Assaymentioning

confidence: 99%

In vitro anticancer evaluation of 5-fluorouracil lipid nanoparticles using B16F10 melanoma cell lines

Shenoy

Gude²,

Murthy

2013

Int Nano Lett

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The present study is aimed to investigate the formulation and in vitro anticancer activities of solid lipid nanoparticles (SLNs) of 5-fluorouracil (5-FU) prepared using glyceryl monostearate (GMS) and cetyl palmitate (CP) by hot homogenization method. The lipids were selected based on the partition coefficient of 5-FU in lipids. The lipid nanoparticles were optimized for process and formulation parameters. The optimized nanoparticles were characterized for their zeta potential, morphology, release kinetics, and anticancer activity. Higher entrapments were achieved using a combination of emulsifiers. The zeta potential of the optimized CP and GMS SLN formulation were −8.26 and −9.35 mV, respectively. Both the optimized formulations were spherical. The in vitro release studies of SLNs of both the lipid carriers followed Peppas-Korsenmeyer equation when carried out at pH 3.5 and 7.4. The chemosensitivity assay carried out in B16F10 cell lines revealed that CP SLNs had better cytotoxicity than 5-FU solution and GMS SLNs at 48 h of incubation. Subtoxic concentration of 5-FU-loaded CP SLNs (0.12 μg/mL) possessed comparable antimigrational activity, colony inhibition activity, and cytopathic as that of 5-FU solution effects. The results indicated that encapsulating 5-FU in CP would be a promising delivery system for delivering 5-FU.

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“…As compared to morphine, hydromorphone has certain advantages in clinical settings, such as lesser nausea and vomiting [26], and lower histamine release [27]. To demonstrate its protective effect, we used hydromorphone to reduce oxidative stress-related injury [28].…”

Section: Discussionmentioning

confidence: 99%

The effects of hydromorphone on astrocytic responses in cerebral ischemia

et al. 2016

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Background: Ischemic insult during operation could cause ischemic-reperfusion injuries in brain and memory impairments. Total intravenous anesthesia (TIVA) is preferred in brain surgery to promote the use of motor evoked potential monitoring and the use of opioids is common in TIVA. However there were few studies about ischemic protective effect of opioids to astrocytes. Methods: We used astrocytes, which were derived from human brain. We divided groups by conditioning period; i) pre-culture, ii) post-culture, or iii) pre + post-culture. All groups were treated 100 nM hydromorphone. We measured reactive oxygen species (ROS) by flow cytometry with 2',7'-dichloroflurorescin diacetate. Then ROS in astrocytes which treated by opioid receptor antagonist were measured after treating 100 nM hydromorphone. Results: ROS was reduced in hydromorphone treated group, as compared to the control group (only tert-butyl hydroperoxide [TBH] treated). There was no difference in pre-conditioned group and post-conditioned group. However, ROS was much more reduced in pre + post-conditioned group compared to pre-conditioned only or post-conditioned only group. Furthermore each selective -, δ-and κ-opioid receptor antagonists partially negated the effect of hydromorphone. Conclusions: This study provides evidence that hydromorphone has both preconditioning and postconditioning effects on TBHinduced oxidative stress. Furthermore we proved each μ-, δ-and κ-opioid receptor relates to protective mechanism of hydromorphone to astrocytes. (Anesth Pain Med 2016; 11: 23-27)

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Influences of opioids and nanoparticles on in vitro wound healing models

Cited by 79 publications

References 71 publications

Modulation of Peripheral Sensory Neurons by the Immune System: Implications for Pain Therapy

Modulation of Peripheral Sensory Neurons by the Immune System: Implications for Pain Therapy

In vitro anticancer evaluation of 5-fluorouracil lipid nanoparticles using B16F10 melanoma cell lines

The effects of hydromorphone on astrocytic responses in cerebral ischemia

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