Influences of over winter conditions on denitrification and nitrous oxide-producing microorganism abundance and structure in an agricultural soil amended with different nitrogen sources

Tatti, Enrico; Goyer, Claudia; Chantigny, Martin H.; Wertz, Sophie; Zebarth, Bernie J.; Burton, David L.; Filion, Martin

doi:10.1016/j.agee.2013.10.021

Cited by 48 publications

(23 citation statements)

References 63 publications

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“…This may reflect greater snow depth (up CT denitrifier community structures in response to tillage regime were observed at every sampling date in two consecutive winters. This is in contrast to a previous study that reported that tillage changed the structure of soil denitrifiers only in specific months during the winter [54]. In the current study, differences in denitrifier community structure induced by tillage management during the NGS were observed at every sampling date, suggesting that they persisted during winter.…”

Section: Overwinter Trends Of Abundance and Structure Of Denitrifier contrasting

confidence: 85%

“…An increase in N 2 O emissions in frozen soils at time other than the spring freeze-thaw cycles was observed in boreal forests [32], pastures [57] and agricultural soils [53,54]. It is not possible to define the exact conditions triggering the N 2 O emission rates in our study, given the limited number of sampling dates.…”

Section: Gaseous Emissions During Wintermentioning

confidence: 77%

“…In the second set of jars, 10 % of the headspace volume was replaced with compressed air to quantify actual N 2 O and carbon dioxide (CO 2 ) emission rates. Details on gas sampling and measurements using gas chromatograph are described by Tatti et al [54]. After 3-h incubation and N 2 O and CO 2 gas sampling, the jars were opened and soil was sub-sampled to determine soil volume.…”

Section: Gaseous Emissionsmentioning

confidence: 99%

“…nosZ transcript numbers were assessed directly on 500 pg of RNA samples, using the SuperScript III Platinum One-Step qRT-PCR kit (Invitrogen), as described by Henderson et al [23]. Standard curves were obtained using three replicates of serial dilutions of linearized plasmids containing cloned denitrifier sequences: the nirS, nirK and nosZ sequences from Pseudomonas stutzeri (425 bp), Alcaligenes faecalis (165 bp) and Pseudomonas brassicacearum (239 bp), respectively, were used [54]. No template controls (NTC) were included for each primer sets.…”

Section: Quantitative Pcrmentioning

confidence: 99%

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Tillage Management and Seasonal Effects on Denitrifier Community Abundance, Gene Expression and Structure over Winter

et al. 2015

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Tillage effects on denitrifier communities and nitrous oxide (N2O) emissions were mainly studied during the growing season. There is limited information for the non-growing season, especially in northern countries where winter has prolonged periods with sub-zero temperatures. The abundance and structure of the denitrifier community, denitrification gene expression and N2O emissions in fields under long-term tillage regimes [no-tillage (NT) vs conventional tillage (CT)] were assessed during two consecutive winters. NT exerted a positive effect on nirK and nosZ denitrifier abundance in both winters compared to CT. Moreover, the two contrasting managements had an opposite influence on nirK and nirS RNA/DNA ratios. Tillage management resulted in different denitrifier community structures during both winters. Seasonal changes were observed in the abundance and the structure of denitrifiers. Interestingly, the RNA/DNA ratios were greater in the coldest months for nirK, nirS and nosZ. N2O emissions were not influenced by management but changed over time with two orders of magnitude increase in the coldest month of both winters. In winter of 2009-2010, emissions were mainly as N2O, whereas in 2010-2011, when soil temperatures were milder due to persistent snow cover, most emissions were as dinitrogen. Results indicated that tillage management during the growing season induced differences in denitrifier community structure that persisted during winter. However, management did not affect the active cold-adapted community structure.

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Section: Overwinter Trends Of Abundance and Structure Of Denitrifier contrasting

confidence: 85%

Section: Gaseous Emissions During Wintermentioning

confidence: 77%

Section: Gaseous Emissionsmentioning

confidence: 99%

Section: Quantitative Pcrmentioning

confidence: 99%

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Tillage Management and Seasonal Effects on Denitrifier Community Abundance, Gene Expression and Structure over Winter

et al. 2015

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“…Indeed, snow cover changes the energy budget of the soil surface by increasing the albedo, reducing the soil heat flux and the surface roughness, and altering soil temperatures and water content profiles. In addition, recent studies show that agricultural soil biological communities continue to function during the non-growing season in northern regions, even though soil temperatures may remain below 08C for several weeks to several months (Pelster et al 2013;Tatti et al 2014). The impact of snow cover simulation on model outputs was assessed by Je´go et al (2014) with STICS with equations and approaches proposed by Trnka et al (2010) and modified by Je´go et al (2014) for simulating snow cover dynamics.…”

Section: Future Improvements Required For Northern Climatesmentioning

confidence: 99%

Simulating forage crop production in a northern climate with the Integrated Farm System Model

et al. 2015

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. 2015. Simulating forage crop production in a northern climate with the Integrated Farm System Model. Can. J. Plant Sci. 95: 745Á757. Whole-farm simulation models are useful tools for evaluating the effect of management practices and climate variability on the agroenvironmental and economic performance of farms. A few process-based farm-scale models have been developed, but none has been evaluated in northern regions with boreal and hemiboreal climates characterized by a short growing season and a long period with snow cover. The study objectives were to calibrate the grass sub-model of the Integrated Farm System Model (IFSM) and evaluate its predictions of yield and nutritive value of timothy and alfalfa, grown alone or in a mixture, using experimental field data from across Canada, and to assess IFSM's predictions of yield of major annual crops grown on dairy farms in eastern Canada using regional yield data from two contrasting regions. Several timothy and alfalfa datasets combining sites, years, harvests, and N fertilization rates were used to calibrate and evaluate the model. For timothy and alfalfa, the model's accuracy was globally satisfactory in predicting dry matter yield and neutral detergent fiber concentration with a normalized root mean square error (NRMSE) B30%. For N uptake, the scatter was a bit larger, especially for timothy (NRMSE 049%), mainly because of a small range in the measured data. The model's accuracy for predicting the yield of annual crops was generally good, with an NRMSE B30%. Adding timothy and alfalfa to the grass sub-model of IFSM and verifying the model's performance for annual crops confirmed that IFSM can be used in northern regions of North America. In addition, the model was able to simulate the yield and nutritive value of a timothyÁalfalfa mixture, which is the most common perennial mixture used in Canada.Key words: Integrated Farm System Model, timothy, alfalfa, mixture, dairy farm, Canada Je´go, G., Rotz, C. A., Be´langer, G., Tremblay, G. F., Charbonneau, E. et Pellerin, D. 2015. Simulation de la production fourrage`re en climat nordique avec le mode`le IFSM (Integrated Farm System Model). Can. J. Plant Sci. 95: 745Á757. Les mode`les qui permettent de simuler le fonctionnement des exploitations agricoles sont des outils utiles pour e´valuer l'effet des pratiques agricoles et de la variabilite´du climat sur la performance agro-environnementale et e´conomique de ces exploitations. Quelques mode`les me´canistes et fonctionnant a`cette e´chelle ont e´te´de´veloppe´s au cours des dernie`res anne´es, mais aucun n'a e´te´e´value´dans les re´gions nordiques au climat bore´al ou semi-bore´al caracte´rise´s par une saison de croissance courte et une longue pe´riode avec couvert de neige. Les objectifs de cette e´tude e´taient d'une part de calibrer le sous-mode`le « prairies » du mode`le IFSM « Integrated Farm system Model » et d'e´valuer la performance du mode`re pour pre´dire le rendement et la valeur nutritive de la fle´ole des pre´s et de la luzerne, cultive´es seules ou ...

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Availability of dissolved organic carbon drives differences in microbial nitrogen‐cycling processes between two sites with cover crops interseeded into corn

Curtright,

Renner,

Tiemann

2023

Soil Science Soc of Amer J

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Interseeding cover crops into corn has been proposed as a technique to extend the cover crop growing window, but interseeded cover crops may reduce N availability and compete with corn for available nutrients. To assess N‐cycling dynamics in soils where cover crops have been interseeded into corn for one or two years, plots were established in duplicate at two sites in Michigan with differing edaphic properties. Annual ryegrass (Lolium multiflorum Lam.), crimson clover (Trifolium incarnatum L.), oilseed radish (Raphanus sativus L.), or a mixture of ryegrass and clover were interseeded into corn at the V3 or V6 stages of corn growth and compared to control plots that did not receive cover crops. We measured active C and N pools during the growing season and after harvest as well as potential activities of microbially mediated nutrient‐cycling processes via extracellular enzymes, nitrification, and denitrification. We found that after two years, interseeded cover crops had little to no effect on active pools of C and N or on microbial nutrient‐cycling activities, but cover crop growth was severely limited in some site‐years. However, we observed major differences in these parameters between sites, with finer‐textured soils exhibiting increased dissolved organic C availability and greater peptidase activity compared to coarser‐textured soils. Our results reveal important spatial and temporal trends that suggest greater C availability can lower the potential for N loss while maintaining a rapid flux of N through the N cycle.This article is protected by copyright. All rights reserved

show abstract

Influences of over winter conditions on denitrification and nitrous oxide-producing microorganism abundance and structure in an agricultural soil amended with different nitrogen sources

Cited by 48 publications

References 63 publications

Tillage Management and Seasonal Effects on Denitrifier Community Abundance, Gene Expression and Structure over Winter

Tillage Management and Seasonal Effects on Denitrifier Community Abundance, Gene Expression and Structure over Winter

Simulating forage crop production in a northern climate with the Integrated Farm System Model

Availability of dissolved organic carbon drives differences in microbial nitrogen‐cycling processes between two sites with cover crops interseeded into corn

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