Influences of Regulation of miR-126 on Inflammation,Th17/Treg Subpopulation Differentiation, and Lymphocyte Apoptosis through Caspase Signaling Pathway in Sepsis

Zou, Qi; Yang, Mei; Yu, Man; Liu, Cheng

doi:10.1007/s10753-020-01298-7

Cited by 22 publications

(18 citation statements)

References 35 publications

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“…After transfection of miR-126 mimics and inhibitors, we found that the expression of miR-126 was changed in T lymphocytes (Fig. 3 a), and the differentiation was changed of Th17/Treg, which was consistent with our previous study, Induction of Th17/Treg differentiation through apoptosis pathway can change the inflammatory factors in septic rats [ 20 ]. We analyzed the Th17 and Treg in peripheral blood of septic rats after miR-126 intervention.…”

Section: Resultssupporting

confidence: 90%

miR-126 ameliorates multiple organ dysfunction in septic rats by regulating the differentiation of Th17/Treg

Zou

Liu

et al. 2022

Mol Biol Rep

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Background The most common cause of death in sepsis is MODS. We hope that miR-126 can regulate the differentiation of Th17/Treg, reduce the infiltration of inflammatory factors in peripheral blood and various organs and tissues, and improve organ function and prognosis in sepsis. Methods and results Septic rat model was established by cecal perforation and ligation. miR-126 mimic and inhibitor were used to intervene sepsis. The experimental results showed that miR-126 mimic reduced the differentiation of Th17 and increased the differentiation of Treg in septic rats, resulting in the TNF-α, IL-6 and IL-17 were decreased in peripheral blood, the infiltration levels of TNF-α, IL-6 and IL-17 were decreased in lung, liver and kidney, the tissue damage degree of lung, liver and kidney were weakened, and the corresponding histopathological score decreased. Finally, the survival rate of septic rats was increased. However, after using miR-126 inhibitor, the levels of inflammatory factors and the degree of multiple organ injury in septic rats increased in varying degrees, and the prognosis of septic rats was worse. Conclusion This study confirmed that miR-126 can regulate the differentiation of Th17/Treg, change the infiltration of inflammatory factors in peripheral blood, lung, liver and kidney of septic rats, alleviate MODS, and improve the organ function and prognosis of septic rats.

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Section: Resultssupporting

confidence: 90%

miR-126 ameliorates multiple organ dysfunction in septic rats by regulating the differentiation of Th17/Treg

Zou

Liu

et al. 2022

Mol Biol Rep

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“…Anti-CD28 reduced CLP-induced splenocytes apoptosis T lymphocyte subsets differentiation and lymphocytes apoptosis played an important role in septic rats [26,27]. The proportion of spleen cell apoptosis was signi cantly increased in CLP mice, with reduced nucleus number, irregular shape of the nucleus, state of condensation or nuclear fragments appear, while anti-CD28 relieved such spleen apoptosis signi cantly (Fig.…”

Section: Patient Characteristics and Comparisonmentioning

confidence: 88%

CD28 Monoclonal Antibody Improve Sepsis Mortality by Amending Th17/Treg Balance

Wu¹,

Dai²,

Xie³

et al. 2021

Preprint

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Background: Sepsis is still developing exorbitantly high mortality. In response to microbial molecules, T cell activation plays a crucial role in sepsis’s initial innate immune reactions. The imbalance between CD4+IL-17+ T helper cells (Th17) and CD4+CD25+ regulatory T cells (Treg) participates in sepsis progression. CD28 signaling pathway was essential for the expression of inflammatory cytokines related to Th17, and play a crucial role in the maintenance of Treg. We investigated the correlations of the balance between Th17 and Treg to prognosis in sepsis patients and influence of anti-CD28 antibody on the ratio of Th17 to Treg in sepsis mice.Methods: 60 sepsis patients' baseline conditions were recorded, and the expressions of inflammatory factors in the peripheral blood and levels of procalcitonin (PCT) were detected. Peripheral blood mononuclear cells (PBMCs) were separated, subtypes of T cells and related biomarkers were measured by Fluorescence-activated cell sorting (FACS). Furthermore, the relationship between the above indicators and patients’ condition scoring (APACHEⅡand SOFA) and ICU hospitalization time were analyzed. To investigate effects of CD28 on the balance of Th17 between Treg, anti-CD28 antibody was intraperitoneal administrated to cecal ligation and puncture (CLP) mice. Results: Compared with septic patients who stay in ICU more than 14 days, the Th17/Treg ratio of patients with ICU hospitalization of fewer than 14 days was significantly lower. The sepsis patients with higher expression of CD28 in peripheral blood lymphocytes have lower APACHE II and SOFA scores. Moreover, the expression of CD28 was significantly higher in sepsis patients than that of healthy donors. After administration of CD28 monoclonal antibody, 7-day mortality and clinical score were significantly improved in septic mice, with splenocyte Th17/Treg ratio decreased. CD28 antibody alleviates the expression of pro-inflammatory factors and spleen injury related to apoptosis. Conclusions: Th17/Treg ratio revealed septic patient severity and can be used as a predictor of ICU stay. CD28 monoclonal antibody could improve 7-day mortality of septic mice by decreasing T cell apoptosis and amending the ratio of Th17/Treg.Trial registration: CHEC2019-133, CTR20191855. Registered 27 August 2019

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“…Interestingly, miR-126, miR-132, miR-221, and miR-625-3p were also connected with the antibody titers or serum-cytokine levels after vaccination in our regression analyses. miR-126 inhibits VCAM-1 expression in endothelial cells, thereby reducing leukocyte adherence to endothelial cells 29 , and miR-126 is known to be increased in sepsis 39 . miR-126-5p, which is produced from the same pre-miR-126 RNA as miR-126 is, binds to capsase-3 and prevents dimerization of the caspase 40 .…”

Section: Discussionmentioning

confidence: 99%

Circulating extracellular vesicle microRNAs associated with adverse reactions, proinflammatory cytokine, and antibody production after COVID-19 vaccination

et al. 2022

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AbstractmRNA-based vaccines have been used globally to eradicate the coronavirus-disease 2019 (COVID-19) pandemic. Vaccine efficacy and adverse reactions depend on immune responses, such as proinflammatory cytokine production and lymphocyte activation. We conducted a prospective cohort study to investigate relationships among specific antibody titers, adverse reactions, proinflammatory cytokine production, and immune-regulatory microRNA (miRNA) levels in serum extracellular vesicles (EVs) after COVID-19 vaccination (BNT162b2). Local adverse reactions after the second dose, such as local pain and swelling, were less correlated with those of systemic symptoms, such as fever and muscle pain, whereas serum TNF-α levels were associated with systemic adverse reactions and with specific antibody titers. Interestingly, EV miR-92a-2-5p levels in sera were negatively correlated with degrees of adverse reactions, and EV miR-148a levels were associated with specific antibody titers. Our data suggest a potential of circulating EV miRNAs as biomarkers for vaccine efficacy and adverse reactions.

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Influences of Regulation of miR-126 on Inflammation,Th17/Treg Subpopulation Differentiation, and Lymphocyte Apoptosis through Caspase Signaling Pathway in Sepsis

Cited by 22 publications

References 35 publications

miR-126 ameliorates multiple organ dysfunction in septic rats by regulating the differentiation of Th17/Treg

miR-126 ameliorates multiple organ dysfunction in septic rats by regulating the differentiation of Th17/Treg

CD28 Monoclonal Antibody Improve Sepsis Mortality by Amending Th17/Treg Balance

Circulating extracellular vesicle microRNAs associated with adverse reactions, proinflammatory cytokine, and antibody production after COVID-19 vaccination

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