Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

Nourozi, Elnaz; Hosseini, Bahman; Hassani, Abbas

doi:10.14720/aas.2016.107.1.05

Cited by 18 publications

(8 citation statements)

References 24 publications

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“…A specific infection period was required by A. rhizogenes for attachment and transfer of its T‐DNA into the plant cell. There were some similar trends reported in Arachis hypogaea and Agastache foeniculum . Actually, short infection period exhibited the low rate of hairy root induction because of insufficient time for bacterial infection.…”

Section: Resultssupporting

confidence: 75%

“…There were some similar trends reported in Arachis hypogaea and Agastache foeniculum. 37,38 Actually, short infection period exhibited the low rate of hairy root induction because of insufficient time for bacterial infection. Long infection period was not effective probably because of hypertonic conditions or hyper-activation of defense mechanism that may damage the cells.…”

Section: Effect Of Inoculation Period On Hairy Root Induction and Daimentioning

confidence: 99%

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Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

Beigmohamadi

Movafeghi

Jafari

et al. 2019

Biotechnology Progress

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Plumbago europaea L. is the main source of plumbagin which is a well‐known pharmacological active compound. In this investigation, genetically transformed roots of P. europaea were obtained by improving some factors affecting the efficiency of Agrobacterium rhizoigenes‐mediated transformation such as explant type, A. rhizoigenes strain, bacterial infection period, co‐cultivation period and acetosyringone concentration. The leaf, hypocotyl and stem explants from in vitro grown plantlets were infected with bacterial strains (A4, ATCC15834, MSU440 and A13). The highest transformation rate of 69.3% was achieved after 7–9 days by inoculating A. rhizogenes MSU440 strain onto the 3‐week‐old stem explants followed by a co‐cultivation period of 2 days on a medium containing 100 μM acetosyringone. To investigate the existence of the rolB gene, polymerase chain reaction was carried out using specific primers. Effects of growth media (MS, 1/2 MS, MS‐B5 and ½ MS‐B5), different sucrose concentrations and illumination on biomass production and plumbagin biosynthesis in P. europaea hairy root cultures were analyzed using stem explants after infection with MSU440 strain. ½ MS‐B5 liquid medium containing 30 g L−1 sucrose incubated in the dark resulted in the efficient biomass production of transformed hairy roots (12.5 g fresh weight, 1.8 g dry weight) with 3.2 mg g−1 DW plumbagin accumulation. This procedure provides a framework for large‐scale cultivation of hairy roots for plumbagin production. This is the first report describing the establishment of P. europaea hairy root culture with special emphasis on plumbagin production.

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Section: Resultssupporting

confidence: 75%

Section: Effect Of Inoculation Period On Hairy Root Induction and Daimentioning

confidence: 99%

Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

Beigmohamadi

Movafeghi

Jafari

et al. 2019

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…Factors that influence successful transformation in plant tissue and hairy root induction include genotype, species, age, type of plant tissue (Sevon et al 2002 ), type of Agrobacterium strain, density of the bacterial suspension (Park and Facchini 2000 ), time duration of infection and media composition (Nourozi et al 2016 ). A number of chemicals may also promote these processes, e.g., acetosyringone (Joubert et al 2002 ).…”

Section: Resultsmentioning

confidence: 99%

Optimizing culture conditions for establishment of hairy root culture of Semecarpus anacardium L.

2017

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Semecarpus anacardium L. is a tree species which produces secondary metabolites of medicinal importance. Roots of the plant have been traditionally used in folk medicines. Different strains of Agrobacterium rhizogenes (A4, ATCC15834 and LBA 9402) were used for induction of hairy roots in in vitro grown tissues of the plant. Hairy root initiation was observed after 25–30 days of infection. Optimum transformation frequency of 61% was achieved on leaf explants with ATCC15834 strain. Infection time of 30 min resulted in greater transformation frequency compared to 10 and 20 min, respectively. The hairy roots cultured in growth regulator-free semi-solid woody plant medium differentiated into callus. Whole shoots infected with ATCC 15834 were found to produce more transformants upon co-cultivation for 4 (65%) and 5 (67%) days. Induction of hairy roots in stem explants infected with ATCC 15834 was lower (52%) compared to leaves (62%) after 4 days of co-cultivation. In A4 and LBA9402 strains transformation efficiency was 49 ± 2.8% and 36 ± 5.7% in shoots after 4 days of co-cultivation. Transformation frequency was higher in ATCC15834 strain, irrespective of explants. The hairy roots of S. anacardium elongated slowly upon transfer to half-strength liquid medium. After 3–4 passages in liquid medium slender hairy roots started differentiating which were separated from the original explants. Visible growth of the roots was observed in hormone-free liquid medium after 2–3 months of culturing. Polymerase chain reaction with gene-specific primers from rol A, B and C genes confirms the positive transformation events.

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“…In this study, a reduction in 50% of components in MS medium increased the biomass production of radish roots as observed in Dracocephalum moldavica (Jeżyna et al 2013). Among the different culture media tested (MS, 1/2 MS, and B5), the maximum growth rate (1.61 g FW 50 ml −1 ) of hairy roots was obtained in 1/2 MS medium using leaf explants of Agastache foeniculum (Nourozi et al 2016). Similar results were also reported in hairy root cultures of Fagopyrum tataricum where 1/2 MS media favored biomass accumulation (fresh weight/dry weight) within 20 days of culture (Bae et al 2013).…”

Section: Discussionmentioning

confidence: 98%

Elite hairy roots of Raphanus sativus (L.) as a source of antioxidants and flavonoids

et al. 2018

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An efficient protocol for hairy root induction in radish was established by optimizing several parameters that affect the efficiency of -mediated transformations. Explants wounded using sterile hypodermic needle, infected with suspension (0.6 OD) for 10 min and co-cultivated in 1/2 MS medium containing acetosyringone (100 µM) for 2 days displayed maximum percentage of hairy root induction using MTCC 2364 (77.6%) and MTCC 532 (67.6%). On further experiments with MTCC 2364 initiated hairy roots, maximum biomass accumulation (fresh weight = 9.50 g; dry weight = 1.48 g) was achieved in liquid 1/2 MS medium supplemented with 87.6 mM sucrose after 40 days of culture. Transgenic state of hairy roots of MTCC 2364 was confirmed by polymerase chain reaction using and-specific primers. The MTCC 2364-induced hairy roots produced higher amount of phenolic (33.0 mg g), flavonoid (48.0 mg g), and quercetin (114.8 mg g) content compared to auxin-induced roots of non-transformed radish. Furthermore, the results of ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl assay confirmed that the antioxidant activity of MTCC 2364 root extracts was improved when compared to auxin-induced roots of non-transformed radish. The present study offers a new insight in radish for production of phenolics and flavonoids (quercetin) using -mediated hairy root induction.

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Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

Cited by 18 publications

References 24 publications

Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

Optimizing culture conditions for establishment of hairy root culture of Semecarpus anacardium L.

Elite hairy roots of Raphanus sativus (L.) as a source of antioxidants and flavonoids

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