Foundation: Diseases associated with the circulatory system are the main causes of worldwide morbidity and mortality, implying the need for vascular implants. Thus, the production of vascular biomaterials has shown to be promising alternatives to therapies used in studies and research related to vascular physiology. Objectives: The present project aims at the artificial development of blood vessels through the recellularization of vascular scaffolds derived from bovine placental vessels. Methods: The chorioallantoic surface of the bovine placenta was used to produce decellularized biomaterials. For recellularization, 2.5 x 10 4 endothelial cells were seeded above each decellularized vessel fragment during three or seven days, when culture were interrupted, and fragments fixed for cell attachment analysis. Decellularized and recellularized biomaterials were evaluated by basic histology, scanning electron microscopy and immunohistochemistry.Results: the decellularization process produced vessels that maintained natural structure and elastin content, also no cells and gDNA reminiscent was observed. Also, endothelial precursor cells attached to lumen and external surface of the decellularized vessel. Conclusion: our results show a possibility in future uses of this biomaterial in cardiovascular medicine, such as for development of engineered vessels.