Influência do estresse calórico na produção in vitro de oócitos e embriões de vacas Holandesas de alta produtividade

Berling, Francieli; Castro, Fernanda Cavallari de; Oliveira, Ana Carolina dos Santos

doi:10.1590/1809-6891v23e-71852p

Cited by 2 publications

(2 citation statements)

References 42 publications

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“…It has also been shown that the given exposure time (6 h) to the temperature of 41 °C disturbs the oxidative balance of the oocyte and cumulus cells [ 41 ]. Numerous studies [ 40 , 41 , 42 , 46 ] show that exposure of oocytes to high temperatures, both in vivo and in vitro, reduces the ability to fertilize and develop the embryo [ 40 , 41 , 42 , 46 ]. Another consistent study by Ispada et al [ 42 ] showed that 14 h exposure of IVM oocytes to a temperature of 41 °C reduces the ability of the oocyte to cleave and develop into the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization

Wrzecińska

Kowalczyk

Kordan

et al. 2023

IJMS

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The main problem in dairy herds is reproductive disorders, which are influenced by many factors, including temperature. Heat stress reduces the quality of oocytes and their maturation through the influence of, e.g., mitochondrial function. Mitochondria are crucial during oocyte maturation as well as the process of fertilization and embryonic development. Disturbances related to high temperature will be increasingly observed due to global warming. In present studies, we have proven that exposure to high temperatures during the cleaving of embryos statistically significantly (at the level of p < 0.01) reduces the percentage of oocytes that cleaved and developed into blastocysts eight days after insemination. The study showed the highest percentage of embryos that underwent division in the control group (38.3 °C). The value was 88.10 ± 6.20%, while the lowest was obtained in the study group at 41.0 °C (52.32 ± 8.40%). It was also shown that high temperature has a statistically significant (p < 0.01) effect on the percentage of embryos that developed from the one-cell stage to blastocysts. The study showed that exposure to a temperature of 41.0 °C significantly reduced the percentage of embryos that split relative to the control group (38.3 °C; 88.10 ± 6.20%). Moreover, it was noted that the highest tested temperature limits the development of oocytes to the blastocyst stage by 5.00 ± 9.12% compared to controls (33.33 ± 7.10%) and cleaved embryos to blastocysts by 3.52 ± 6.80%; the control was 39.47 ± 5.40%. There was also a highly significant (p < 0.0001) effect of temperature on cytoplasmic ROS levels after 6 and 12 h IVM. The highest level of mitochondrial ROS was found in the group of oocytes after 6 h IVM at 41.0 °C and the lowest was found in the control group. In turn, at 41.0 °C after 12 h of IVM, the mitochondrial ROS level had a 2.00 fluorescent ratio, and the lowest in the group was 38.3 °C (1.08). Moreover, with increasing temperature, a decrease in the expression level of both LC3 and SIRT1 protein markers was observed. It was proved that the autophagy process was impaired as a result of high temperature. Understanding of the cellular and molecular responses of oocytes to elevated temperatures will be helpful in the development of heat resistance strategies in dairy cattle.

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Section: Discussionmentioning

confidence: 99%

Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization

Wrzecińska

Kowalczyk

Kordan

et al. 2023

IJMS

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“…The embryo transfer (ET) is a technique where embryos from genetically selected individuals are transferred to a recipient that will carry and give birth to the offspring (Vieira & Pazzim, 2021). This technology allows for an increase in the number of animals derived from selected crosses, enhancing genetic improvement (Berling et al, 2022). The success in ET depends on the presence of a corpus luteum at the time of embryo implantation, ensuring the reproductive physiology continues the gestation process.…”

Section: Embryo Transfermentioning

confidence: 99%

Mesenchymal stem cells in animal reproduction: sources, uses and scenario

Oliveira,

Braga,

Miranda

et al. 2024

Braz. J. Vet. Med.

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Studies regarding mesenchymal stem cells turned up in the 1960's and this cell type created a great number of questions about its functions and applicability in science and medicine. When used with therapeutic intent, these cells present an inclination to migrate to sites of injury, inflammation or disease, where they secrete bioactive factors that stimulates the synthesis of new tissue. In this context, studies using rodents reported that MSCs promoted positive effects in the ovarian function in mice with premature aging of follicular reserve. In female bovines, experimental stem cell-based therapies have been used to either generate new oocytes with in vitro quality or stimulate such action in vivo. It is also reported, that the intraovarian application of mesenchymal stem cells generates a greater production of embryos in vitro and the production of early and expanded blastocysts. Additionally, analysis of ovarian tissue in animal subjected to treatment showed an increase in the number of developing follicles. Nevertheless, the treatments involving stem cells with different modes of application, different sources and different species were able to act on the hormonal, tissue, cellular and metabolic levels, generating positive results in the recovery and improvement of ovarian functions.

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Influência do estresse calórico na produção in vitro de oócitos e embriões de vacas Holandesas de alta produtividade

Cited by 2 publications

References 42 publications

Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization

Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization

Mesenchymal stem cells in animal reproduction: sources, uses and scenario

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