Influencing factors and formation mechanism of CaCO3 precipitation induced by microbial carbonic anhydrase

Zheng, Tao; Qian, Chunxiang

doi:10.1016/j.procbio.2019.12.018

Cited by 90 publications

(23 citation statements)

References 40 publications

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“…The self-healing schematic diagram of concrete cracks is shown in Figure , and the specific steps of mineralization product deposition are as follows. ,, Under the catalysis of CA produced by the microorganism, the hydration reaction of CO 2 was greatly improved, and a great amount of H + and HCO 3 – could be produced in the crack zone solution: In the alkali solution of the concrete crack zone, HCO 3 – reacted with OH – to form CO 3 2– and H 2 O: Since the microbial vegetative cells were negatively charged, positively charged Ca 2+ would be accumulated on their surfaces The microbial vegetative cells acted as nucleation sites to promote the deposition of mineralized products: …”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the growth of spores was represented by an OD 600 value. The enzyme activity change could be tested by a colorimetric method; for the specific test method, one could refer to previous research, 42 which was mainly based on the different catalytic efficiencies of different enzyme activities. The enzyme activity was indirectly reflected by the amount of p-nitrophenol.…”

Section: Methodsmentioning

confidence: 99%

“…The self-healing schematic diagram of concrete cracks is shown in Figure 25, and the specific steps of mineralization product deposition are as follows. 42,70,71 (1) Under the catalysis of CA produced by the microorganism, the hydration reaction of CO 2 was greatly improved, and a great amount of H + and HCO 3 − could be produced in the crack zone solution:…”

Section: Protection Effect Of Carrier On Sporesmentioning

confidence: 99%

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Effect and Mechanism of Encapsulation-Based Spores on Self-Healing Concrete at Different Curing Ages

Zheng

Zhang

et al. 2020

ACS Appl. Mater. Interfaces

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It has become an intelligent and environmental protection method to repair concrete cracks based on microbial-induced calcium carbonate precipitation (MICP). However, due to the high-alkali environment in concrete, even the microbial spores with strong alkali resistance find it difficult to survive for a long time, which affects the long-term self-healing effect of concrete cracks. In this paper, low-alkali sulfo-aluminate cement (SC) was used as a carrier to encapsulate spores, and the effects of the spore group and microbial group on the basic performances of concrete were studied. Then, the area repair ratio, water permeability, the repair ratio of anti-chloride ion penetration, and ultrasonic velocity were used to evaluate the self-healing efficiency of cracks, and the self-healing effects of two kinds of microbial self-healing agents on concrete cracks with different curing ages were further studied. Moreover, the growth, enzyme activity, and microbial morphologies of spores with and without encapsulation immersed in the simulated pore solution of cement-based materials at different times were studied to discuss the protective effect of the carrier on spores. Compared with the reference group, the results showed that the addition of two microbial self-healing agents would slightly affect the basic performances of concrete, but both were within the control range of concrete materials. For the early-age cracks, the two kinds of microbial self-healing agents could achieve a good self-healing effect, but for the later-age cracks, the concrete cracks of the microbial group could still be repaired well, while the self-healing effect of the spore group was greatly reduced. Moreover, the white precipitates generated at the crack mouth were all calcite CaCO3. In addition, the self-healing mechanism of different microbial self-healing agents on concrete cracks was discussed carefully. This study provides a new idea and method for the engineering application of microbial self-healing concrete.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Protection Effect Of Carrier On Sporesmentioning

confidence: 99%

See 1 more Smart Citation

Effect and Mechanism of Encapsulation-Based Spores on Self-Healing Concrete at Different Curing Ages

Zheng

Zhang

et al. 2020

ACS Appl. Mater. Interfaces

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“…In a study by Zheng, it was shown that the highest activity of CA was at 30°C, and the highest calcification rate of CaCO 3 was at a pH level of 9. Therefore, CA can accelerate the hydration of CO 2 in HCO 3- and react with OH − and Ca 2+ to form CaCO 3 precipitation in an alkaline environment and in the presence of a calcium source ( Zheng, 2019 ). Moon et al investigated the effect of different calcium sources with different solubilities and CA enzymes on carbonate mineralization.…”

Section: Enzyme-induced Calcium Carbonate Precipitationmentioning

confidence: 99%

A Review of Enzyme-Induced Calcium Carbonate Precipitation Applicability in the Oil and Gas Industry

Alarifi

Mustafa

Omarov

et al. 2022

Front. Bioeng. Biotechnol.

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Enzyme-induced calcium carbonate precipitation (EICP) techniques are used in several disciplines and for a wide range of applications. In the oil and gas industry, EICP is a relatively new technique and is actively used for enhanced oil recovery applications, removal of undesired chemicals and generating desired chemicals in situ, and plugging of fractures, lost circulation, and sand consolidation. Many oil- and gas-bearing formations encounter the problem of the flow of sand grains into the wellbore along with the reservoir fluids. This study offers a detailed review of sand consolidation using EICP to solve and prevent sand production issues in oil and gas wells. Interest in bio-cementation techniques has gained a sharp increase recently due to their sustainable and environmentally friendly nature. An overview of the factors affecting the EICP technique is discussed with an emphasis on the in situ reactions, leading to sand consolidation. Furthermore, this study provides a guideline to assess sand consolidation performance and the applicability of EICP to mitigate sand production issues in oil and gas wells.

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“…This widespread enzyme is involved in several physiological processes related to CO 2 and carbonates, such as respiration, CO 2 transport, and photosynthesis [21]. Nevertheless, few studies have been conducted on prokaryotes concerning the direct involvement of this enzyme in CaCO 3 precipitation [11,22]. CA promotes the interconversion of CO 2 into HCO 3 − (Equation ( 6)) and increases pH by releasing carbonates and bicarbonates in large quantities [3,4].…”

Section: Introductionmentioning

confidence: 99%

New Biocalcifying Marine Bacterial Strains Isolated from Calcareous Deposits and Immediate Surroundings

et al. 2021

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Marine bacterial biomineralisation by CaCO3 precipitation provides natural limestone structures, like beachrocks and stromatolites. Calcareous deposits can also be abiotically formed in seawater at the surface of steel grids under cathodic polarisation. In this work, we showed that this mineral-rich alkaline environment harbours bacteria belonging to different genera able to induce CaCO3 precipitation. We previously isolated 14 biocalcifying marine bacteria from electrochemically formed calcareous deposits and their immediate environment. By microscopy and µ-Raman spectroscopy, these bacterial strains were shown to produce calcite-type CaCO3. Identification by 16S rDNA sequencing provided between 98.5 and 100% identity with genera Pseudoalteromonas, Pseudidiomarina, Epibacterium, Virgibacillus, Planococcus, and Bhargavaea. All 14 strains produced carbonic anhydrase, and six were urease positive. Both proteins are major enzymes involved in the biocalcification process. However, this does not preclude that one or more other metabolisms could also be involved in the process. In the presence of urea, Virgibacillus halodenitrificans CD6 exhibited the most efficient precipitation of CaCO3. However, the urease pathway has the disadvantage of producing ammonia, a toxic molecule. We showed herein that different marine bacteria could induce CaCO3 precipitation without urea. These bacteria could then be used for eco-friendly applications, e.g., the formation of bio-cements to strengthen dikes and delay coastal erosion.

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Influencing factors and formation mechanism of CaCO3 precipitation induced by microbial carbonic anhydrase

Cited by 90 publications

References 40 publications

Effect and Mechanism of Encapsulation-Based Spores on Self-Healing Concrete at Different Curing Ages

Effect and Mechanism of Encapsulation-Based Spores on Self-Healing Concrete at Different Curing Ages

A Review of Enzyme-Induced Calcium Carbonate Precipitation Applicability in the Oil and Gas Industry

New Biocalcifying Marine Bacterial Strains Isolated from Calcareous Deposits and Immediate Surroundings

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