Due to the unique geographical location and historical culture, the traditional houses in the southern region of Anhui Province, China (South Anhui) have different indoor environments. In summer and winter, this study adopted a field survey, questionnaire survey, and statistical analysis to carry out a comprehensive field survey on Xixinan Village in South Anhui, and selected a typical traditional residence in the village to evaluate its indoor environment status. The final results show that the overall indoor environment of the traditional houses in South Anhui was awful, including the indoor thermal environment, with high temperature and humidity in summer and cold and humidity in winter. Additionally, the indoor light environment with dim light still had much room for improvement, while the indoor air quality and sound environment were relatively excellent. In addition, this study determined that the neutral temperatures of residents are 15.5 °C and 28.7 °C in winter and summer, respectively, and the comfort range of indoor light intensity is 752.6–1252.5 lx, which determines the adjustment range of indoor environmental parameters for residents’ comfort needs. This paper’s research methods and results provide a reference for the study of residential indoor environments in other regions with similar climatic conditions as South Anhui, and a theoretical basis for architects and engineers to enhance the indoor environment of traditional houses in this region.