Influenza A virus hemagglutinin and neuraminidase act as novel motile machinery

Sakai, Tatsuya; Nishimura, Shin Ichiro; Naito, Tadasuke; Saito, Mineki

doi:10.1038/srep45043

Cited by 127 publications

(158 citation statements)

References 41 publications

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“…This peak decreases with added NA inhibitor and is therefore ascribed to receptor cleaving by NA. Block et al claim that the lateral diffusion of the glycolipids to which inuenza binds remains signicantly faster than the NA-dependent motility that was reported by Sakai et al 15,17 It is interesting to note the differences in setup and observed mobility between these four publications. Both Block et al and Sakai et al used an egg-adapted inuenza strain with HA and NA from the IAV strain Aichi/2/68 (H3N2).…”

Section: Motility Of Influenza Virusmentioning

confidence: 87%

“…[50][51][52][53] It is likely that the organization of HA and NA has an inuence on the way their functions cooperate. Motility of inuenza was rst reported by Sakai et al 15 They tracked the motion of inuenza viruses over fetuin-modied glass with total internal reection uorescence microscopy (TIRF). When adding an NA inhibitor, the movement of the viruses was completely blocked.…”

Section: Motility Of Influenza Virusmentioning

confidence: 92%

“…Sinai. 5,16 It is therefore remarkable that Block et al report a motion that is up to two orders of magnitude faster than Sakai et al, whereas Vahey and Fletcher reported a motion that is an order of magnitude slower than Sakai et al 15,17,18 Unlike De Haan et al, who reported that NA contributes to the association of viruses, 16 Block et al report an increase of association rate with added NA inhibitor. 17 We think it likely that the differences described above are primarily due to the different structures of the surfaces that were used in these studies.…”

Section: Motility Of Influenza Virusmentioning

confidence: 97%

“…1,3,14 Recently, several groups have described a new function of NA in imparting motility of the virus on a surface. [15][16][17][18] Sakai et al…”

Section: P H (Erik) Hammingmentioning

confidence: 99%

“…Both Block et al and Sakai et al used an egg-adapted inuenza strain with HA and NA from the IAV strain Aichi/2/68 (H3N2). 15,17 Vahey and Fletcher used HA and NA from A/WSN/33 (H1N1). 18 De Haan et al used the HA and NA from the same strain for its lower NA activity and used A/Puerto Rico/8/34 Mount Sinai (H1N1) to demonstrate the removal of receptors.…”

Section: Motility Of Influenza Virusmentioning

confidence: 99%

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Influenza as a molecular walker

2020

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Section: Motility Of Influenza Virusmentioning

confidence: 87%

Section: Motility Of Influenza Virusmentioning

confidence: 92%

Section: Motility Of Influenza Virusmentioning

confidence: 97%

“…1,3,14 Recently, several groups have described a new function of NA in imparting motility of the virus on a surface. [15][16][17][18] Sakai et al…”

Section: P H (Erik) Hammingmentioning

confidence: 99%

Section: Motility Of Influenza Virusmentioning

confidence: 99%

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Influenza as a molecular walker

2020

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Arbidol: The current demand, strategies, and antiviral mechanisms

Kang,

Shi,

2023

Immunity Inflam & Disease

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BackgroundHigh morbidity and mortality of influenza virus infection have made it become one of the most lethal diseases threatening public health; the lack of drugs with strong antiviral activity against virus strains exacerbates the problem.MethodsTwo independent researchers searched relevant studies using Embase, PubMed, Web of Science, Google Scholar, and MEDLINE databases from its inception to December 2022.ResultsBased on the different antiviral mechanisms, current antiviral strategies can be mainly classified into virus‐targeting approaches such as neuraminidase inhibitors, matrix protein 2 ion channel inhibitors, polymerase acidic protein inhibitors and other host‐targeting antivirals. However, highly viral gene mutation has underscored the necessity of novel antiviral drug development. Arbidol (ARB) is a Russian‐made indole‐derivative small molecule licensed in Russia and China for the prevention and treatment of influenza and other respiratory viral infections. ARB also has inhibitory effects on many other viruses such as severe acute respiratory syndrome coronavirus 2, Coxsackie virus, respiratory syncytial virus, Hantaan virus, herpes simplex virus, and hepatitis B and C viruses. ARB is a promising drug which can not only exert activity against virus at different steps of virus replication cycle, but also directly target on hosts before infection to prevent virus invasion.ConclusionARB is a broad‐spectrum antiviral drug that inhibits several viruses in vivo and in vitro, with high safety profile and low resistance; the antiviral mechanisms of ARB deserve to be further explored and more high‐quality clinical studies are required to establish the efficacy and safety of ARB.

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A Dynamic, Supramolecular View on the Multivalent Interaction between Influenza Virus and Host Cell

Overeem

Vries

Huskens

2021

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Understanding how influenza viruses traverse the mucus and recognize host cells is critical for evaluating their zoonotic potential, and for prevention and treatment of the disease. The surface of the influenza A virus is covered with the receptor‐binding protein hemagglutinin and the receptor‐cleaving enzyme neuraminidase, which jointly control the interactions between the virus and the host cell. These proteins are organized in closely spaced trimers and tetramers to facilitate multivalent interactions with sialic acid‐terminated glycans. This review shows that the individually weak multivalent interactions of influenza viruses allow superselective binding, virus‐induced recruitment of receptors, and the formation of dynamic complexes that facilitate molecular walking. Techniques to measure the avidity and receptor specificity of influenza viruses are reviewed, and the pivotal role of multivalent interactions with their emergent properties in crossing the mucus and entering host cells is discussed. A model is proposed for the initiation of cell entry through virus‐induced receptor clustering. The multivalent interactions of influenza viruses are maintained in a dynamic regime by a functional balance between binding and cleaving.

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Influenza A virus hemagglutinin and neuraminidase act as novel motile machinery

Cited by 127 publications

References 41 publications

Influenza as a molecular walker

Influenza as a molecular walker

Arbidol: The current demand, strategies, and antiviral mechanisms

A Dynamic, Supramolecular View on the Multivalent Interaction between Influenza Virus and Host Cell

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