Influenza A virus hemagglutinin prevents extensive membrane damage upon dehydration

Iriarte-Alonso, Maiara A.; Bittner, Alexander M.; Chiantia, Salvatore

doi:10.1016/j.bbadva.2022.100048

Cited by 6 publications

(5 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They showed that without the presence of stabilizing agents such as trehalose, upon abrupt dehydration and subsequent rehydration, membranes lose their integrity and severe structural damage in the form of holes, aggregates and delimitation of membrane is observed 30 . Similar results were reported by Iriarte-Alonso et al for single component DOPC membrane, where abrupt dehydration led to the formation of multiple defects and holes and consequently loss of membrane continuity 31 . Many attempts have been made to maintain the structure of desiccated lipid membranes and prevent them from rapid vesiculation.…”

Section: Introductionsupporting

confidence: 90%

“…3A and S4A throughout the entire rehydration process the integrity of lipid membranes remained undamaged. Iriarte-Alonso et al reported that membranes composed of pure DOPC upon abrupt and complete dehydration and rehydration loose their structural arrangement and membrane continuity, resulting in abundance of various defects and holes, which exposed the bare support 31 . This gives even more importance to the dehydration method presented in our research, which allows the membrane and lipids to adapt to the slowly changing hydration state of the membrane.…”

Section: Nanoscale Structural Changes Under Dehydration Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Nanoscale structural response of biomimetic cell membranes to controlled dehydration

Krok¹,

Franquelim

Chattopadhyay³

et al. 2023

Preprint

View full text Add to dashboard Cite

Although cell membranes in physiological conditions exist in excess of water, there is a number of biochemical processes, such as adsorption of biomacromolecules or membrane fusion events, that require partial or even complete, transient dehydration of lipid membranes. Even though the dehydration process is crucial for understanding all fusion events, still little is known about the structural adaptation of the lipid membranes when their interfacial hydration layer is perturbed. Here, we introduce the study on the nanoscale structural reorganization of the phase-separated, supported lipid bilayers (SLBs) under a wide range of hydration conditions. Model lipid membranes were characterized with the combination of fluorescence microscopy and atomic force microscopy, and crucially, without applying any chemical or physical modifications, that so far have been considered to be indispensable for maintaining the membrane integrity upon dehydration. We revealed that decreasing hydration state of the membrane leads to an enhanced mixing of lipids characteristic for the liquid-disordered (Ld) phase with those forming liquid-ordered (Lo) phase. This is associated with a 2-fold decrease in the hydrophobic mismatch between the Ld and Lo lipid phases and a 3-fold decrease of line tension for the completely desiccated membrane. Importantly, the observed changes in the hydrophobic mismatch, line tension, and miscibility of lipids are fully reversible upon subsequent rehydration of the membrane. These findings provide deeper insights into the fundamental processes such as cell-cell fusion that require partial dehydration at the interface of two membranes.

show abstract

Section: Introductionsupporting

confidence: 90%

Section: Nanoscale Structural Changes Under Dehydration Conditionsmentioning

confidence: 99%

Nanoscale structural response of biomimetic cell membranes to controlled dehydration

Krok¹,

Franquelim

Chattopadhyay³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…4 seconds) to get a quasi-constant and maximized signal 64 . Each data set was analysed in MATLAB using custom-written code 65 . The analysis directly provided lipid diffusion in monolayers ( D m ) and the surface concentration C of TF-PC molecules in the monolayer.…”

Section: Methodsmentioning

confidence: 99%

A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

Mandal,

Brandt,

Tempra

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Cellular membranes are composed of lipids typically organized in a double-leaflet structure. Interactions between these two leaflets - often referred to as interleaflet coupling - play a crucial role in various cellular processes. Despite extensive study, the mechanisms governing such interactions remain incompletely understood. Here, we investigate the effects of interleaflet coupling from a specific point of view, i.e. by comparing diffusive dynamics in bilayers and monolayers, focusing on potential lipid-specific interactions between opposing leaflets. Through quantitative fluorescence microscopy techniques, we characterize lipid diffusion and mean molecular area in monolayers and bilayers composed of different lipids. Our results suggest that the observed decrease in bilayer lipid diffusion compared to monolayers depends on lipid identity. Furthermore, our analysis suggests that lipid acyl chain structure and spatial configuration at the bilayer may strongly influence interleaflet interactions and dynamics in bilayers. These findings provide insights into the role of lipid structure in mediating interleaflet coupling and underscore the need for further experimental investigations to elucidate the underlying mechanisms.

show abstract

“…Iriarte-Alonso et al reported that pure DOPC membranes lose their structural arrangement and membrane continuity upon abrupt and complete dehydration and rehydration, resulting in an abundance of various defects and holes exposing the bare support. 31 This gives even more importance to the dehydration method presented in our research, which allows the membrane and lipids to adapt to the slowly changing hydration state of the membrane. The high resolution images of individual domains revealed increased mixing of the L o and L d phases as shown in Fig.…”

Section: Nanoscale Structural Changes Under Dehydration Conditionsmentioning

confidence: 96%

“…30 Similar results were reported by Iriarte-Alonso et al for single-component DOPC membranes, where abrupt dehydration resulted in the formation of multiple defects and holes, and consequently loss of membrane continuity. 31 Many attempts have been made to preserve the structure of desiccated lipid membranes and prevent them from rapid vesiculation. These include the use of saccharides, which are known to increase the spacing between lipids in the dry state and thus prevent them from collapsing, 30,32 the modification of lipid head groups to improve the interactions between the lipids and the solid support [33][34][35] or the introduction of physical confinement, which prevents the interfacial peeling force from causing destructive membrane delamination.…”

Section: Introductionmentioning

confidence: 99%

Nanoscale structural response of biomimetic cell membranes to controlled dehydration

Krok,

Franquelim,

Chattopadhyay

et al. 2024

Nanoscale

View full text Add to dashboard Cite

show abstract

Influenza A virus hemagglutinin prevents extensive membrane damage upon dehydration

Cited by 6 publications

References 73 publications

Nanoscale structural response of biomimetic cell membranes to controlled dehydration

Nanoscale structural response of biomimetic cell membranes to controlled dehydration

A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

Nanoscale structural response of biomimetic cell membranes to controlled dehydration

Contact Info

Product

Resources

About