2024
DOI: 10.57187/s.3503
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Influenza transmission dynamics quantified from RNA in wastewater in Switzerland

Sarah Nadeau,
Alexander J. Devaux,
Claudia Bagutti
et al.

Abstract: INTRODUCTION: Influenza infections are challenging to monitor at the population level due to many mild and asymptomatic cases and similar symptoms to other common circulating respiratory diseases, including COVID-19. Methods for tracking cases outside of typical reporting infrastructure could improve monitoring of influenza transmission dynamics. Influenza shedding into wastewater represents a promising source of information where quantification is unbiased by testing or treatment-seeking behaviours. METHODS: … Show more

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Cited by 8 publications
(1 citation statement)
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“…Subsequently, these samples were transported on ice to a central laboratory (Eawag, Dübendorf, Switzerland) for processing. The processing steps involved total nucleic acid extraction from 40 ml samples by using the Wizard Enviro Total Nucleic Acid Extraction Kit (CN A2991, Promega Corporation, USA) with an elution volume of 80 µl as described in Nadeau et al 22 . Following this, inhibitor removal was performed by using OneStep PCR Inhibitor Removal columns (CN D6030, Zymo Research, USA).…”
Section: Sampling and Processing Of Wastewatermentioning
confidence: 99%
“…Subsequently, these samples were transported on ice to a central laboratory (Eawag, Dübendorf, Switzerland) for processing. The processing steps involved total nucleic acid extraction from 40 ml samples by using the Wizard Enviro Total Nucleic Acid Extraction Kit (CN A2991, Promega Corporation, USA) with an elution volume of 80 µl as described in Nadeau et al 22 . Following this, inhibitor removal was performed by using OneStep PCR Inhibitor Removal columns (CN D6030, Zymo Research, USA).…”
Section: Sampling and Processing Of Wastewatermentioning
confidence: 99%