Influenza vaccine format mediates distinct cellular and antibody responses in human immune organoids

Kastenschmidt, Jenna M.; Sureshchandra, Suhas; Jain, Aarti; Hernandez-Davies, Jenny E.; Assis, Rafael de; Wagoner, Zachary W.; Sorn, Andrew M.; Mitul, Mahina Tabassum; Benchorin, Aviv I.; Levendosky, Elizabeth; Ahuja, Gurpreet S.; Zhong, Qian; Trask, Douglas K.; Boeckmann, Jacob; Nakajima, Rie; Jasinskas, Algimantas; Saligrama, Naresha; Davies, D. Huw; Wagar, Lisa E.

doi:10.1016/j.immuni.2023.06.019

Cited by 26 publications

(14 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…109,110 It is thus possible to speculate that the transient induction of interferons that follows influenza viruses encounter, or the influenza vaccination may affect the capacity of the host to control the infection with SARS-CoV-2. [111][112][113] Our data demonstrate that the protective effect of influenza vaccination fades over time. Influenza vaccines have been shown to induce interferons to different extents, [111][112][113] suggesting that the broad capacity of interferons, produced in response to influenza vaccine, to "interfere" with the spread and/or replication of viruses may explain the cross-protection against SARS-CoV-2 observed in individuals that were recently vaccinated against influenza.…”

Section: The Possible Mechanisms Of Actionmentioning

confidence: 63%

“…Our data demonstrate that the protective effect of influenza vaccination fades over time. Influenza vaccines have been shown to induce interferons to different extents, 111–113 suggesting that the broad capacity of interferons, produced in response to influenza vaccine, to “interfere” with the spread and/or replication of viruses may explain the cross‐protection against SARS‐CoV‐2 observed in individuals that were recently vaccinated against influenza.…”

Section: Discussionmentioning

confidence: 99%

“…Interferons might be a key factor in the interaction between influenza vaccination and SARS‐CoV‐2 infection; different types of interferons are produced in the lungs of COVID‐19 patients based on disease severity and location along the respiratory tract 109,110 . It is thus possible to speculate that the transient induction of interferons that follows influenza viruses encounter, or the influenza vaccination may affect the capacity of the host to control the infection with SARS‐CoV‐2 111–113 …”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Vaccination against influenza viruses reduces infection, not hospitalization or death, from respiratory COVID‐19: A systematic review and meta‐analysis

Pontiroli,

Scovenna,

Carlini

et al. 2024

Journal of Medical Virology

View full text Add to dashboard Cite

Severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) causes COVID‐19 and has brought a huge burden in terms of human lives. Strict social distance and influenza vaccination have been recommended to avoid co‐infections between influenza viruses and SARS‐CoV‐2. Scattered reports suggested a protective effect of influenza vaccine on COVID‐19 development and severity. We analyzed 51 studies on the capacity of influenza vaccination to affect infection with SARS‐CoV‐2, hospitalization, admission to Intensive Care Units (ICU), and mortality. All subjects taken into consideration did not receive any anti‐SARS‐CoV‐2 vaccine, although their status with respect to previous infections with SARS‐CoV‐2 is not known. Comparison between vaccinated and not‐vaccinated subjects for each of the four endpoints was expressed as odds ratio (OR), with 95% confidence intervals (CIs); all analyses were performed by DerSimonian and Laird model, and Hartung‐Knapp model when studies were less than 10. In a total of 61 029 936 subjects from 33 studies, influenza vaccination reduced frequency of SARS‐CoV‐2 infection [OR plus 95% CI = 0.70 (0.65−0.77)]. The effect was significant in all studies together, in health care workers and in the general population; distance from influenza vaccination and the type of vaccine were also of importance. In 98 174 subjects from 11 studies, frequency of ICU admission was reduced with influenza vaccination [OR (95% CI) = 0.71 (0.54−0.94)]; the effect was significant in all studies together, in pregnant women and in hospitalized subjects. In contrast, in 4 737 328 subjects from 14 studies hospitalization was not modified [OR (95% CI) = 1.05 (0.82−1.35)], and in 4 139 660 subjects from 19 studies, mortality was not modified [OR (95% CI) = 0.76 (0.26−2.20)]. Our study emphasizes the importance of influenza vaccination in the protection against SARS‐CoV‐2 infection.

show abstract

Section: The Possible Mechanisms Of Actionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Vaccination against influenza viruses reduces infection, not hospitalization or death, from respiratory COVID‐19: A systematic review and meta‐analysis

Pontiroli,

Scovenna,

Carlini

et al. 2024

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…Others used cryopreserved tonsil cells cultured in plates as an in vitro immunization system. In this work, tonsil organoids could respond to both recall and neo antigens and support key GC features, including antigen-specific somatic hypermutation, affinity maturation, and class switching 25,27 . Similarly to our in vitro model, they induced immune responses against neoantigens and previously encountered antigens with a significant increase in GC B cells.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, Purwada et al developed a B cell follicle organoid where murine naive B cells differentiate into antigen-specific germinal center (GC) B cells [22][23][24] . Others demonstrated the potential of human tonsil single-cells for developing in vitro systems that resemble lymphoid organs and support adaptive immunity [25][26][27] . However, it remains elusive to what extent reconstituted cell cultures mimic the physiologic 3D spatial organization and cell-cell interactions found in ex vivo tissues and whether this affects their predictivity as models.…”

Section: Introductionmentioning

confidence: 99%

Tonsil explants as a human in vitro model to study immune responses to vaccines

Berger,

Bonaiti,

Muraro

et al. 2023

Preprint

View full text Add to dashboard Cite

Vaccination is one of the most effective infection prevention strategies. Viruses with high mutation rates -such as influenza- escape vaccine-induced immunity and represent significant challenges to vaccine design. Influenza vaccine strain selection is based on circulating strains and immunogenicity testing in animal models with limited predictive outcomes for vaccine effectiveness in humans. Here, we developed a human in vitro vaccination model using human tonsil tissue explants cultured in 3D perfusion bioreactors to be utilized as a platform to test and improve vaccines. Tonsils cultured in bioreactors showed higher viability, metabolic activity, and more robust immune responses than those in static cultures. Our in vitro vaccination system responded to various premanufactured vaccines, protein antigens, and antigen combinations. In particular, a multivalent in vitro immunization with three phylogenetically distant H3N2 influenza strains induced higher B cell differentiation, T cell activation, and antibody cross-reactivity compared to combinations with more related strains. Moreover, we demonstrate the capacity of our in vitro model to generate de novo humoral immune responses. Thus, our study indicates that perfusion-cultured tonsil tissue is a valuable human in vitro model for immunology research with potential application in vaccine candidate selection.

show abstract