Influenza Virosomes as Antigen Delivery System

Moser, Christian; Amacker, Mario

doi:10.1007/978-1-4614-5380-2_14

Cited by 4 publications

(5 citation statements)

References 75 publications

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“…The virosome surface is responsible for a stronger immune stimulation, probably due to the addition of HA and NA, generating not only OVA-specific T cells but also HA-specific ones and therefore enhancing the overall immune response. Pre-existing immunity to influenza virus has no negative effects in humans (57, 58) but shows to be an advantage as both antigen-specific and influenza-specific T helper cells stimulate B cells and CTLs for strong immune response (34, 59). Mouse studies revealed that immunization with influenza virus resulted in higher antibody titers when administered with virosomes as adjuvants (60).…”

Section: Discussionmentioning

confidence: 99%

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4+ T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen

Blom

Amacker²,

Dijk

et al. 2017

Front. Immunol.

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Pulmonary administration of biomimetic nanoparticles loaded with antigen may represent an effective strategy to directly modulate adaptive immune responses in the respiratory tract. Depending on the design, virosomes may not only serve as biomimetic antigen carriers but are also endowed with intrinsic immune-stimulatory properties. We designed fluorescently labeled influenza-derived virosomes and liposome controls coupled to the model antigen ovalbumin to investigate uptake, phenotype changes, and antigen processing by antigen-presenting cells exposed to such particles in different respiratory tract compartments. Both virosomes and liposomes were captured by pulmonary macrophages and dendritic cells alike and induced activation in particle-bearing cells by upregulation of costimulatory markers such as CD40, CD80, CD86, PD-L1, PD-L2, and ICOS-L. Though antigen processing and accumulation of both coupled and soluble antigen was similar between virosomes and liposomes, only ovalbumin-coupled virosomes generated a strong antigen-specific CD4+ T cell proliferation. Pulmonary administrated antigen-coupled virosomes therefore effectively induced adaptive immune responses and may be utilized in novel preventive or therapeutic approaches in the respiratory tract.

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Section: Discussionmentioning

confidence: 99%

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4+ T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen

Blom

Amacker²,

Dijk

et al. 2017

Front. Immunol.

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“…Designing novel vaccine candidates that closely mimic specific pathogens without being pathogenic themselves remains a key challenge for influenza vaccine development (Kapoor et al ., ). Virosomes are tightly controllable virus‐like particles suitable for vaccine formulation (Moser & Amacker, ).…”

Section: Discussionmentioning

confidence: 99%

An H1-H3 chimeric influenza virosome confers complete protection against lethal challenge with PR8 (H1N1) and X47 (H3N2) viruses in mice

et al. 2014

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Annual health threats and economic damages caused by influenza virus are still a main concern of the World Health Organization and other health departments all over the world. An influenza virosome is a highly efficient immunomodulating carrier mimicking the natural antigen presentation pathway and has shown an excellent tolerability profile due to its biocompatibility and purity. The major purpose of this study was to construct a new chimeric virosome influenza vaccine containing hemagglutinin (HA) and neuraminidase (NA) proteins derived from the A/PR/8/1934 (H1N1) (PR8) and A/X/47 (H3N2) (X47) viruses, and to evaluate its efficacy as a vaccine candidate in mice. A single intramuscular vaccination with the chimeric virosomes provided complete protection against lethal challenge with the PR8 and X47 viruses. The chimeric virosomes induced high IgG antibody responses as well as hemagglutination inhibition (HAI) titers. HAI titers following the chimeric virosome vaccination were at the same level as the whole inactivated influenza vaccine. Mice immunized with the chimeric virosomes displayed considerably less weight loss and exhibited significantly reduced viral load in their lungs compared with the controls. The chimeric virosomes can be used as an innovative vaccine formulation to confer protection against a broad range of influenza viruses.

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“…Viruses are obligate intracellular parasites as they are necessarily dependent upon specific host cells for their survival. This principle leads to the development of a drug delivery system that mimics the viral pattern of cellular infection [1]. Virosomes are composed of a phospholipid bilayer with the viral surface glycoproteins protruding from the surface of these vesicles [2][3][4].…”

Section: Applications Of Virosome Technologymentioning

confidence: 99%

“…Cancer chemotherapeutic agents, for instance, can be delivered specifically to the tumors by labeling the virosomes with antibodies [9,10]. Virosomes have shown to effectively transport macromolecules including drugs, nucleic acids and proteins to various cell types including hepatocytes, erythrocytes, immune cells and glioma cells [1,[11][12][13].…”

Section: Applications Of Virosome Technologymentioning

confidence: 99%

“…Moreover, the identification, uptake and representation of the antigen incorporated in the virosome by the relevant antigen presenting cells helps in stimulating the immune system. As a result efficient regulatory and effector immune responses are generated [1,15]. They initiate both cell mediated and humoral arms of immune system [39,40].…”

Section: Virosomes As Immunopotentiating Agentsmentioning

confidence: 99%

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Virosomes-Hybrid Drug Delivery Systems

Kazi¹

2013

J Antivir Antiretrovir

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The development of safe and effective models for the delivery of any prophylactic or therapeutic agent remains an uphill task for pharmaceutical formulation developers. Drug molecules, nucleic acids, carbohydrates, proteins and a variety of other biological and chemical entities are used for attaining pharmacological benefits. However, the major challenge remains in the delivery of these agents to the specific site of action in a time-efficient manner. Among the many drug delivery systems developed, the nano scale technology of virosomes tends to present an established system of delivering the therapeutic agents to the site of pharmacological action. Virosomes are lipid bilayer, unilamellar structures that present viral protein on their surface. They are safe, biocompatible and biodegradable structures that can achieve ideal pharmacological profile once administered into the body. Tissue targeting, immune activation and potentiation are the chief advantages that make them efficient prophylactic and therapeutic agents. The review presents the biopharmaceutical applications of virosomes and the immunological and pharmaceutical considerations that make them efficient agents for targeting spatiotemporal parameters in the body.

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Influenza Virosomes as Antigen Delivery System

Cited by 4 publications

References 75 publications

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4+ T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen

Pulmonary Delivery of Virosome-Bound Antigen Enhances Antigen-Specific CD4+ T Cell Proliferation Compared to Liposome-Bound or Soluble Antigen

An H1-H3 chimeric influenza virosome confers complete protection against lethal challenge with PR8 (H1N1) and X47 (H3N2) viruses in mice

Virosomes-Hybrid Drug Delivery Systems

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