2021
DOI: 10.1101/2021.03.21.436327
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Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single Particle Tracking Microscopy

Abstract: In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization. We demonstrate that off-center sampling at locations predicted by Fisher information utilizes photons most efficiently. When performing localization in a single dimension, optimized off-center sampling patterns gave doubled precision compared to uniform sampling. A ~20% increase in precision compared to uniform sampling… Show more

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