According to Landauer's principle, erasing a memory requires an average work of at least kT ln 2 per bit. Recent experiments have confirmed this prediction for a one-bit memory represented by a symmetric double-well potential. Here, we present an experimental study of erasure for a memory encoded in an asymmetric double-well potential. Using a feedback trap, we find that the average work to erase can be less than kT ln 2. Surprisingly, erasure protocols that differ subtly give measurably different values for the asymptotic work, a result we explain by showing that one protocol is symmetric with the respect to time reversal, while the other is not. The differences between the protocols help clarify the distinctions between thermodynamic and logical reversibility.Introduction. Landauer's principle states that erasing a one-bit memory requires an average work of at least kT ln 2 [1, 2], with the lower bound achieved in the quasistatic limit. It plays a key role in sharpening our understanding of the second law of thermodynamics and of the interplay between information and thermodynamics, an issue first raised Maxwell [3], developed in important contributions by Szilard [4] and Bennett [5] but also subject to a long, sometimes confused discussion [6]. Recent experiments have confirmed Landauer's prediction in simple systems: in a one-bit memory represented by a symmetric double-well potential [7, 8], in memory encoded by nanomagnetic bits [9, 10], and even in quantum bits [11]. These successes have helped to create an extended version of stochastic thermodynamics [12, 13] that views information as another kind of thermodynamic resource, on the same footing as heat, chemical energy, and other sources of work [14]. This new way of looking at thermodynamics has led to experimental realizations of information engines ("Maxwell demons") [15][16][17][18].Despite its success in simple situations, Landauer's principle remains untested in more complex cases, such as systems where the symmetry between states is broken. This case, briefly mentioned but not pursued in Landauer's original paper [1], was followed up in later theoretical work [2,[19][20][21][22][23][24][25]. In addition, erasure in asymmetric states can be interpreted as situations where the initial system is out of global thermodynamic equilibrium. Since nonequilibrium settings are ubiquitous in biological systems, it is important to establish basic scenarios in simpler settings to understand more clearly, for example, why common biological systems may not be able to reach ultimate thermodynamic limits [26].Here, we explore the broken-symmetry case experimentally by studying erasure in a memory represented by an asymmetric, double-well potential. While we find broad agreement with the main predictions of theoretical work,