INFRAPOPULATION DYNAMICS OF A WILD STRAIN OF TAENIA CRASSICEPS (WFU) (CESTODA: TAENIIDAE) IN BALB/cJ MICE

Everhart, Mary E.; Kuhn, Raymond; Zelmer, Derek A.

doi:10.1645/ge-3255

Cited by 27 publications

(23 citation statements)

References 50 publications

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“…crassiceps strain WFU was isolated from a wild field mouse (Peromyscus sp.) captured in the state of Michigan in August 1999 and maintained in the laboratory by intraperitoneal passage in female Balb/c mice (Everhart et al 2004).…”

Section: Methodsmentioning

confidence: 99%

Ultrastructure of spermiogenesis and the spermatozoon in Taenia crassiceps strobilae WFU strain (Cestoda, Cyclophyllidea, Taeniidae) from golden hamsters

Willms¹,

Robert²,

Jiménez³

et al. 2004

Parasitol Res

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Strobilae from Taenia crassiceps (WFU strain) were obtained from outbred hamsters (Mesocricetus auratus) by feeding them viable metacestodes maintained by intraperitoneal passage in female Balb/c mice. Mature and gravid proglottids from strobilae were recovered from hamster intestines and fixed for light and electron microscopy. By light microscopy, the expected structure of taeniid proglottids was observed. Ultrastructural analysis of ten proglottids showed that testicular follicles and vas deferens contained filiform spermatids, with a single axoneme, and an elongated helicoidal nucleus inserted between the axoneme and the spiraled cortical microtubules. At the apical cone, a single crest-like body was found and mature spermatids also exhibited transverse intracytoplasmic walls. The morphology and characters of the spermatids in T. crassiceps conform to type III spermiogenesis, which has been described in other taeniids.

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Section: Methodsmentioning

confidence: 99%

Ultrastructure of spermiogenesis and the spermatozoon in Taenia crassiceps strobilae WFU strain (Cestoda, Cyclophyllidea, Taeniidae) from golden hamsters

Willms¹,

Robert²,

Jiménez³

et al. 2004

Parasitol Res

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“…crassiceps cysticerci (WFU strain) were maintained in our laboratory by peritoneal passages in mice (Everhart et al 2004). Other authors generally infect mice with doses of ten non-budding cysticerci (Sciutto et al 1995;Rodriguez-Sosa et al 2002), but we injected 40 ± 2 cysticerci with a 1-2-mm diameter intraperitoneally in each Balb/c female mouse with the purpose of inducing an amplified and fast inflammatory reaction.…”

Section: Parasites Infection and Peritoneal Cellsmentioning

confidence: 99%

Decrease of peritoneal inflammatory CD4+, CD8+, CD19+ lymphocytes and apoptosis of eosinophils in a murine Taenia crassiceps infection

et al. 2010

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After an intraperitoneal infection of mice with Taenia crassiceps metacestodes, peritoneal inflammatory cells labeled with fluoresceinated MoAb anti-mouse were analyzed by flow cytometry. Apoptosis was studied by annexin A/PI, TUNEL assays, DNA laddering, caspase-3 activity, and electron microscopy. An important continuous decrease of CD4+, CD8+ and CD19+ lymphocytes, and an increase of eosinophils and macrophages throughout the observation time were found. Apoptosis of eosinophils was quantified during the observation period with a peak at 6 days post-infection (67.27%). In an additional experiment at 12 days post-infection using TUNEL staining, a high level of apoptosis of eosinophil (92.3%) and a significant decrease of CD4+, CD8+, and CD19+ lymphocytes were confirmed. Caspase-3 activity in peritoneal fluid, peritoneal cells' DNA fragmentation, and apoptosis of eosinophils and monocytes were found. The dramatic decrease of peritoneal inflammatory T and B cells and the high level of apoptosis of inflammatory eosinophils induced in mice by infection with T. crassiceps cysticerci may be important factors of the immunosuppression observed in cysticercosis.

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“…solium cysticerci were dissected from naturally infected pork, washed three times with sterile phosphate-buffered saline (PBS), and stored at −70°C until use. T. crassiceps WFU strain was extracted from the peritoneum of infected BALB/cAnN female mice killed with CO 2 and washed three times with sterile PBS (Everhart et al 2004). …”

Section: Biological Materialsmentioning

confidence: 99%

Characterization of one typical 2-Cys Peroxiredoxin gene of Taenia solium and Taenia crassiceps

2009

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The Taenia genus is capable of living for long periods within its hosts. Reports have shown that this successful establishment is related to its efficient defense mechanisms against host immune response and its high tolerance to oxidative stress. In this work, we describe the genomic sequences of one Taenia solium and Taenia crassiceps typical 2-Cys peroxiredoxins (Ts2-CysPrx, Tc2-CysPrx) genes, which are 94% identical in primary sequence with the typical 2-Cys Prxs catalytic motifs. Both genes have the same genomic architecture, showing a TATA box and Initiator (Inr) sequence in their proximal promoter, two exons split by a 67-bp type III intron and one unique transcription start site located inside the Inr. We show that T. crassiceps cysticerci are highly tolerant to H(2)O(2) presenting a lethal concentration 50 of 3.0 mM and demonstrate that the typical Tc2-CysPrx gene is not induced by H(2)O(2), showing a behavior of an antioxidant housekeeping gene. This study describes for first time the gene structure of a typical 2-Cys Prx in the Taenia genus.

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INFRAPOPULATION DYNAMICS OF A WILD STRAIN OF TAENIA CRASSICEPS (WFU) (CESTODA: TAENIIDAE) IN BALB/cJ MICE

Cited by 27 publications

References 50 publications

Ultrastructure of spermiogenesis and the spermatozoon in Taenia crassiceps strobilae WFU strain (Cestoda, Cyclophyllidea, Taeniidae) from golden hamsters

Ultrastructure of spermiogenesis and the spermatozoon in Taenia crassiceps strobilae WFU strain (Cestoda, Cyclophyllidea, Taeniidae) from golden hamsters

Decrease of peritoneal inflammatory CD4+, CD8+, CD19+ lymphocytes and apoptosis of eosinophils in a murine Taenia crassiceps infection

Characterization of one typical 2-Cys Peroxiredoxin gene of Taenia solium and Taenia crassiceps

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