Infrared imaging of calcified tissue in bone biopsies from adults with osteomalacia

Faibish, Dan; Gomes, Andreia; Boivin, Georges; Binderman, Itzhak; Boskey, Adele L.

doi:10.1016/j.bone.2004.08.019

Cited by 107 publications

(86 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(18) However, to our knowledge, no previous studies have employed FTIRI to investigate bone from patients with ROD. Carbonate is substituted into the hydroxyapatite crystal over time.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Recent studies have indicated that the increased fracture risk in other metabolic bone diseases such as osteoporosis not only may follow from the low bone mass and distorted bone structure but also may be due to altered composition of the bone tissue. (14)(15)(16)(17) The ability of Fourier transform infrared spectroscopic imaging (FTIRI) to characterize changes in the mineral or collagen phase (17)(18)(19)(20) The advantage of the technique is its ability to image and measure the spatial composition of the tissue. FTIRI can be used to identify samples, to characterize unknown materials, and also to determine the quality or consistency of a sample.…”

Section: Introductionmentioning

confidence: 99%

“…In principle, FTIRI may provide a tool for diagnosing certain metabolic bone diseases that alter tissue composition. (16,18) In this study we hypothesize that FTIRI can identify differences in tissue composition between normal bone and bone from patients diagnosed with ROD. The sample material consisted of iliac crest biopsies.…”

mentioning

confidence: 99%

“…In previous literature, most studies have been based on sample sets of 10 or fewer samples per group. (17)(18)(19)(20)27) Hence our sample size of 11 samples per group was considered sufficient.As a conclusion, this study successfully differentiated normal and ROD bone based on Fourier transform IR spectroscopic imaging for the first time. This was conducted using several parameters related to carbonate content (carbonate-to-phosphate and carbonate-to-amide I ratios), as well as the trabecular profile of mineral-to-matrix ratio.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy

Isaksson

Turunen

Rieppo

et al. 2010

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

Renal osteodystrophy alters metabolic activity and remodeling rate of bone and also may lead to different bone composition. The objective of this study was to characterize the composition of bone in high-turnover renal osteodystrophy patients by means of Fourier transform infrared spectroscopic imaging (FTIRI). Iliac crest biopsies from healthy bone (n ¼ 11) and patients with renal osteodystrophy (ROD, n ¼ 11) were used in this study. The ROD samples were from patients with hyperparathyroid disease. By using FTIRI, phosphate-toamide I ratio (mineral-to-matrix ratio), carbonate-to-phosphate ratio, and carbonate-to-amide I ratio (turnover rate/remodeling activity), as well as the collagen cross-link ratio (collagen maturity), were quantified. Histomorphometric analyses were conducted for comparison. The ROD samples showed significantly lower carbonate-to-phosphate ( p < .01) and carbonate-to-amide I ( p < .001) ratios. The spatial variation across the trabeculae highlighted a significantly lower degree of mineralization ( p < .05) at the edges of the trabeculae in the ROD samples than in normal bone. Statistically significant linear correlations were found between histomorphometric parameters related to bone-remodeling activity and number of bone cells and FTIRI-calculated parameters based on carbonate-to-phosphate and carbonate-to-amide I ratios. Hence the results suggested that FTIRI parameters related to carbonate may be indicative of turnover and remodeling rate of bone. ß 2010 American Society for Bone and Mineral Research.KEY WORDS: BONE COMPOSITION; HISTOMORPHOMETRY; FOURIER TRANSFORM; INFRARED; RENAL OSTEODYSTROPHY; CARBONATE B one is a composite material consisting of a mineral phase (hydroxyapatite), an organic phase (collagen), noncollagenous proteins, lipids, and water. (1,2) Metabolic bone disease is a common term referring to a number of abnormalities caused by a broad spectrum of disorders. Most disorders lead to weakening of the bone or impaired bone function and are followed most often by altered bone mechanical properties. Renal osteodystrophy (ROD) is a chronic kidney disease that can be accompanied by different types of bone pathologies. ROD can lead to defective mineralization, altered bone morphology, and/or bone turnover. (3,4) Commonly, it is accompanied by hyperparathyroid disease, which is characterized by increased bone turnover. Patients with renal osteodystrophy may exhibit bone and joint pain, bone deformation, and spontaneous bone fractures. Bone biopsies followed by quantitative histomorphometry prevail as the ''gold standard'' for the diagnosis of renal osteodystrophy. (5)(6)(7) Histomorphometric analysis emphasizes increased amounts of osteoid and erosion surface and a larger amount of bone cells than what is present in normal bone. (8) However, the invasive nature of obtaining the biopsies, the required experience of the pathologists to identify the abnormalities, as well as the lack of standardized normal limits remain problematic.ROD also commonly results in lower bone ...

show abstract

“…(18) However, to our knowledge, no previous studies have employed FTIRI to investigate bone from patients with ROD. Carbonate is substituted into the hydroxyapatite crystal over time.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy

Isaksson

Turunen

Rieppo

et al. 2010

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

show abstract

Introduction to Spectral Imaging, and Applications to Diagnosis of Lymph Nodes

Romeo¹,

Dukor²,

Diem³

2001

Handbook of Vibrational Spectroscopy

View full text Add to dashboard Cite

This chapter introduces the field of vibrational spectroscopic imaging and discusses the advances made to date in terms of biomedical diagnosis. The reader is introduced to the spectral signatures of typical cellular components, and the need for an objective method for cancer diagnosis is discussed. Besides an overview of typical instrumentation and components, this chapter provides a working application, namely the diagnosis of metastatic cancer in lymph nodes. Through this application methods of sample preparation, data acquisition and data preprocessing and analysis are discussed.

show abstract

Infrared and Raman Spectroscopic Imaging

Steiner

2012

Biomedical Imaging

View full text Add to dashboard Cite

Infrared imaging of calcified tissue in bone biopsies from adults with osteomalacia

Cited by 107 publications

References 33 publications

Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy

Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy

Introduction to Spectral Imaging, and Applications to Diagnosis of Lymph Nodes

Infrared and Raman Spectroscopic Imaging

Contact Info

Product

Resources

About