Infrared laser hemotherapy in cerebral ischemia modeling

Abstract

Use of intravenous laser irradiation of blood (ILIB) is considered to be the most effective method of laser therapy and its application is expedient pathogenetically in the ischemic disturbances. The aim of this study is to investigate ILIB influence with infrared laser (IL) with 860 nm wavelength on hemostasis, acid-base status (ABS) of blood in normal rabbits and after modeling of local ischemia of brain (LIB). Experimental cerebral ischemia is characterized by development of hypercoagulation syndrom and metabolic acidosis. ILIB with infrared radiation of 2.0 mW power provokes hypocoagulation in intact animals. Application of ILIB in rabbits after LIB contributes for hemostasis and acidbase status normalizing compared to operated animals. IL radiation with 8,5 mW power results in marked hemostatic activation in all animals. Therefore, beneficial effect of low power laser radiation (LPLR) manifests in narrow power diapason in experimental brain ischemia.

Correction of biochemical and functional disorders in brain ischaemia with laser therapy

“…Laser haemotherapy normalizes oxygen extraction from blood by tissues and improves their oxiganation in conditions of activation of the free radical oxidation owing to HOA increasing and ODC shift leftwards. Application of ILIB leads to pH rising, 11 therefore the change of oxygen-binding properties and drop of H + ions concentration contributes to ODC shift leftwards and HOA increasing under intravenous application of LPLR. Besides, ILIB of operated rabbits contributes reduction of lactate acidosis manifestations due to antihypoxic LPLR effect.…”

“…Besides, ILIB of operated rabbits contributes reduction of lactate acidosis manifestations due to antihypoxic LPLR effect. 11 There is marked ischemic zone restriction here because of tissues microcirculation and revascularization improvement, oxygen deficiency lowering , 40,41 complete elimination of cerebral structures edema, blood antioxidant system activation and cells membrane stabilization. 40,42 In the number of experimental works it has been suggested, that LPLR interaction with components of tissue respiration led to their reactivation and ATP synthesis stimulation as laser light acceptors in human organism appear cytochromoxidase and cytochrome а -а 3 .…”

“…6,11 Subsequent reperfusion provoked hyperoxia in the affected tissue due to the Bohr effect because metabolic acidosis leads to the decrease of HOA and ODC shift rightwards. 16,34 Oxygen is produced in greater than normal amounts what contributes to tissue damage by O 2 species including superoxide radicals, hydrogen peroxide and hydroxyl radicals, which initiate lipid peroxidation and other deleterious oxidation reactions during the reperfusion period.…”

“…It have been demonstrated that the following factors attribute to them: head dermal microhaemodynamics, lipid peroxidation parameters, haemostasis findings and acidbase status of blood. 10,11 The microhaemodynamics (MHD) of skin and brain is estimation criterion of the functional alterations and treatment efficiency in different hypoxic states. It allows concluding that ILIB is expedient pathogenetically in cerebrovascular diseases.…”

Molecular mechanisms of photochemotherapy (Review)

“…However, the change of these properties during phototherapy has been studied less than that of others. Laser blood irradiation helps to improve the acid-base condition of the organism as characterized by a normalization of the pH, an increase in the concentration of bicarbonate ions, and a decrease in the lack of buffering bases compared with the initial data [70,78,81].…”