Infrared photoinduced electrochemiluminescence microscopy of single cells

Descamps, Julie; Zhao, Yiran; Goudeau, Bertrand; Manojlovic, Dragan; Loget, Gabriel; Sojic, Neso

doi:10.1039/d3sc05983a

Cited by 9 publications

(7 citation statements)

References 70 publications

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“…Additionally, cells grown on such semiconductor electrodes were labeled with luminophores and imaged at low anodic potentials. [51] Zhao and co-workers reported also the PECL mapping of hot carrier-driven redox reactions at single photocatalysts. [49] Based on a nanostructured photovoltaic junction, ECL reagents were oxidized without any external power supplies, electrochemical setups, electrodes, or wiring constraints, thus generating light in an all-optical ECL (AO-ECL) configuration.…”

Section: Developments Of Ecl Microscopymentioning

confidence: 99%

Electrochemiluminescence Microscopy

Knežević,

Han,

Liu

et al. 2024

Angewandte Chemie

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Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into an optical microscopy. The orthogonality of the electrochemical trigger and the optical readout distinguishes it from classic microscopy and electrochemical techniques, owing to its near‐zero background, remarkable sensitivity, and absence of photobleaching and phototoxicity. In this review, we summarize the recent advances in ECL imaging technology, emphasising original configurations which enable the imaging of biological entities and the improvement of the analytical properties by increasing the complexity and multiplexing of bioassays. Additionally, mapping the (electro)chemical reactivity in space provides valuable information on nanomaterials and facilitates deciphering ECL mechanisms for improving their performances in diagnostics and (electro)catalysis. Finally, we highlight the recent achievements in imaging at the ultimate limits of single molecules, single photons or single chemical reactions, and the current challenges to translate the ECL imaging advances to other fields such as material science, catalysis and biology.

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Section: Developments Of Ecl Microscopymentioning

confidence: 99%

Electrochemiluminescence Microscopy

Knežević,

Han,

Liu

et al. 2024

Angewandte Chemie

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“…Infrared Photoinduced Electrochemiluminescence Microscopy of Single CellsDescampsJ.ZhaoY.GoudeauB.ManojlovicD.LogetG.SojicN. Descamps, J. Zhao, Y. Goudeau, B. Manojlovic, D. Loget, G. Sojic, N. Chem. Sci.20241520552061 . Application of PECL to the microscopy characterization of cells.…”

Section: Key Referencesmentioning

confidence: 99%

“…(f) Photoluminescence (PL) micrograph (top panel, green color) and positive PECL image (bottom panel, red color) of cells labeled with SA@Ru. Adapted with permission from ref . Copyright 2024 Royal Society of Chemistry.…”

Section: Applicationsmentioning

confidence: 99%

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Light Conversion by Electrochemiluminescence at Semiconductor Surfaces

Zhao,

Descamps,

Léger

et al. 2024

Acc. Chem. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Electrochemiluminescence (ECL) is the electrochemical generation of light.It involves an interfacial charge transfer that produces the excited state of a luminophore at the electrode surface. ECL is a powerful readout method that is widely employed for immunoassays and clinical diagnostics and is progressively evolving into a microscopy technique. On the other hand, photoelectrochemistry at illuminated semiconductors is a field of research that deals with the transfer of photogenerated charge carriers at the solid−liquid interface. This concept offers several advantages such as a considerable lowering of the onset potential required for triggering an electrochemical reaction as well as light addressable chemistry, via the spatial confinement of redox reactions at locally illuminated semiconductor electrodes. The combination of ECL with photoelectrochemistry at illuminated semiconductors is termed photoinduced ECL (PECL). It deals with the triggering of an ECL reaction through the transfer of photogenerated minority charge carriers at the illuminated solid/liquid interface. PECL results in the conversion of incident photons (λ exc ), that are absorbed by the semiconductor photoelectrode to emitted photons (λ PECL ), produced by the ECL reaction. Although demonstrated in the 1970s by Bard et al. in ultradry organic solvents, PECL remained unexplored until the last five years. Nowadays, as a result of the considerable progress achieved in semiconductor photoelectrodes and ECL systems, a large variety of PECL systems can be designed by combining photoelectrode materials with ECL luminophores, making it a versatile tool for light conversion in aqueous media.In this Account, we introduce the fundamentals of ECL and photoelectrochemistry at illuminated semiconductors and review the recent developments in PECL. We discuss the two main PECL light conversion schemes: downconversion (where λ exc < λ PECL ) and upconversion (where λ exc > λ PECL ). Besides, PECL can be used to simplify considerably the common electrochemical setups employed for ECL. Indeed, by engineering the photoelectrode material and carefully considering the reactivity involved for ECL and its counter-reaction, PECL enables the ultimate concept of all-optical ECL (AO-ECL), i.e., ECL generation at an illuminated monolithic device immersed into the electrolyte solution. As discussed in this Account, AO-ECL is an important breakthrough that allows the simplest ECL experimental configuration ever reported, eliminating constraints such as an electrical power supply, wires, electrodes, connections, and specific electrochemical knowledge. As shown at the end of this Account, due to the robustness of recently manufactured PECL systems, several applications can already be envisioned for microscopy, elucidation of solar conversion mechanisms, near-infrared imaging, and bioanalysis.

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“…have reported local visible light (λ exc = 625 to λ ECL = 430 nm) photoconversion with the luminol-H 2 O 2 coreactant ECL system on n -Si protected with a covalently grafted monolayer . PECL has also been reported for imaging single cells and the photoelectrochemical activity of single gold nanoparticles. , IR-to-visible light conversion was proposed in the 70s; however, this was not practically achieved until 2020 with the model [Ru(bpy) 3 ] 2+ -TPrA ECL system on a Si-based metal–insulator–semiconductor (MIS) photoanode (λ exc = 840 to λ ECL = 632 nm) . In the two cited examples, , photogenerated holes simultaneously oxidize the luminophore and the coreactant in the liquid phase and the oxidized species react together to generate the excited state of the luminophore, leading to visible light emission.…”

Section: Introductionmentioning

confidence: 99%

Near-IR Photoinduced Electrochemiluminescence Imaging with Structured Silicon Photoanodes

Zhao,

Sépulveda,

Descamps

et al. 2024

ACS Appl. Mater. Interfaces

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Infrared (IR) imaging devices that convert IR irradiation (invisible to the human eye) to a visible signal are based on solid-state components. Here, we introduce an alternative concept based on light-addressable electrochemistry (i.e., electrochemistry spatially confined under the action of a light stimulus) that involves the use of a liquid electrolyte. In this method, the projection of a near-IR image (λ exc = 850 or 840 nm) onto a photoactive Si-based photoanode, immersed into a liquid phase, triggers locally the photoinduced electrochemiluminescence (PECL) of the efficient [Ru(bpy) 3 ] 2+ -TPrA system. This leads to the local conversion of near-IR light to visible (λ PECL = 632 nm) light. We demonstrate that compared to planar Si photoanodes, the use of a micropillar Si array leads to a large enhancement of local light generation and considerably improves the resolution of the PECL image by preventing photogenerated minority carriers from diffusing laterally. These results are important for the design of original light conversion devices and can lead to important applications in photothermal imaging and analytical chemistry.

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Infrared photoinduced electrochemiluminescence microscopy of single cells

Abstract: Electrochemiluminescence (ECL) is evolving rapidly from a purely analytical technique into a powerful microscopy. Herein, we report the imaging of single cells by photoinduced ECL (PECL; em = 620 nm)...

Cited by 9 publications

References 70 publications

Electrochemiluminescence Microscopy

Electrochemiluminescence Microscopy

Light Conversion by Electrochemiluminescence at Semiconductor Surfaces

Near-IR Photoinduced Electrochemiluminescence Imaging with Structured Silicon Photoanodes

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