Infrared Spectra and the Chain Organization of Erythrocyte Membranes

Chapman, D.; Kamat, V.B.; Levene, R.

doi:10.1126/science.160.3825.314

Cited by 71 publications

(35 citation statements)

References 12 publications

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“…The spectrum of the sonicated and extruded erythrocyte ghost vesicles (N2) shows typical features of biological samples: the stretching vibrations of lipid hydrocarbon chain moiety with a little contribution of -CH 3 groups of protein side chains (at 3050-2700 cm -1 ), carbonyl esters (at 1738 cm -1 ) and the Amide I and Amide II modes of protein peptide bonds at 1656 and 1542 cm -1 , respectively [35]. As additional DPPC is mixed to the ghost vesicle samples, the intensity of the characteristic stretching bands of CH 2 and C=O groups, related to lipid entities, increases accordingly (see N3 and N4 in Fig.6.…”

Section: Ft-ir Spectroscopymentioning

confidence: 99%

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Deák

Mihály

Szigyártó

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Colloidal stabile nanoerythrosomes with 200 nm average diameter were formed from hemoglobin-free erythrocyte ghost membrane via sonication and membrane extrusion. The incorporation of extra lipid (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC), added to the sonicated ghosts, caused significant changes in the thermotropic character of the original membranes. As a result of the increased DPPC ratio the chain melting of the hydrated DPPC system and the characteristic small angle X-ray scattering (SAXS) of the lipid bilayers appeared. Significant morphological changes were followed by transmission electron microscopy combined with freeze fracture method (FF-TEM). After the ultrasonic treatment the large entities of erythrocyte ghosts transformed into nearly spherical nanoerythrosomes with diameters between 100-300 nm and at the same time a great number of 10-30 nm large membrane proteins or protein clusters were dispersed in the aqueous medium. The infrared spectroscopy (FT-IR) pointed out, that the sonication did not cause changes in the secondary structures of the membrane proteins under our preparation conditions. About fivefold of extra lipid -compared to the lipid content of the original membrane -caused homogeneous dispersion of nanoerythrosomes however the shape of the vesicles was not uniform. After the addition of about tenfold of DPPC, monoform and monodisperse nanoerythrosomes became typical. The outer surfaces of these roughly spherical objects were frequently polygonal, consisting of a net of pentagons and hexagons..

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Section: Ft-ir Spectroscopymentioning

confidence: 99%

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Deák

Mihály

Szigyártó

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…Data that have been interpreted as evidence for an interaction between proteins and the fatty acid chains of lipids in bilayers come from recent NMR studies of model membranes (43) and are also compatible with this model . Work on the enzymic conversion of the monounsaturated fatty acid chains of phospholipids into cyclopropane chains 6 2 6 THE JOURNAL OF CELL BIOLOGY • VOLUME 42,1969 are also of interest here . This reaction takes place in bacterial membranes and can be duplicated in vitro with isolated enzymes and lipid bilayers .…”

Section: Re-mentioning

confidence: 99%

“…However, X-ray diffraction evidence indicates that, in the myelin sheath and in isolated membrane preparations (86), the hydrocarbon chains are disordered . NMR (41,212,249), IR (38,42), spin-labeling (125), and differential thermal analysis studies (43,249,251) also point to a disordered state for the hydrocarbon parts of the lipid molecules, at least in some of the few membranes so far studied . While the impedance measurements indicate that the layer with the low dielectric constant covers most of the surface, it should be emphasized that small discontinuities in this layer might not be detected by this technique .…”

Section: Evidence and Arguments Support-ing The Danielli Modelmentioning

confidence: 99%

“…622) . It seems possible that work on the model systems will lead to sufficiently stringent 6 2 8 THE JOURNAL OF CELL BIOLOGY • VOLUME 42,1969 criteria for these or related techniques to allow a quantitative determination of the amount of bilayer actually present in natural membranes . This would be a major step in clarifying the extent to which the bilayer model is relevant for biological membranes .…”

Section: General Evaluation Of the Danielli Modelmentioning

confidence: 99%

“…None of the many experimental results and arguments ad- 6 14 THE JOURNAL OF CELL BIOLOGY • VOLUME 42,1969 vanced in support of a given structure is compelling . Those which presently occupy most of the attention are listed here in the form in which they are usually discussed in the literature .…”

mentioning

confidence: 99%

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Current Models for the Structure of Biological Membranes

Stoeckenius

Engelman

1969

The Journal of Cell Biology

246

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Vascular graft healing. II. FTIR analysis of polyester graft samples from implanted bi-grafts

Lyman¹,

Murray-Wijelath²,

Ambrad–Chalela³

et al. 2001

J. Biomed. Mater. Res.

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FTIR-ATR analysis has shown that the 4-step process for preclotting polyester vascular grafts results in a uniform and reproducible fibrin coating of the polyester fibers. Western blot analyses have shown that FN and VEGF are also present in this fibrin coating. FTIR-ATR analyses of explanted grafts indicate that, while the in vivo healing of these preclotted polyester grafts proceed through the inflammation, proliferation, and remodeling phases of normal wound healing, these phases are modified. Because the fibrin coating provides a nonporous barrier between peri-graft tissue and the flowing blood, these molecular changes are controlled by the interactions of blood-borne constituents with the lumenal surface of the preclotted graft. Also, a well prepared preclotted polyester graft shows a minimal inflammatory response. After implantation, the fibrin preclot is more than 90% gone by the fifth day. However, the proliferation phase, involving synthesis of new protein and polysaccharide materials to replace the fibrin, appears to have begun by the third day. Detection of collagen I in the 5-day explants suggests that the overlapping remodeling phase of healing has begun. Protein and saccharide materials continue to be synthesized and remodeled, and, by the tenth day, collagen IV is detected. By 14-days post-implantation, there is an increase in collagen IV and cellular membrane lipids. Because collagen IV is an indicator of the presence of endothelial cells, some of these cellular membranes must be of endothelial origin. Thus, it appears that FTIR-ATR can be a useful tool in the study of vascular healing.

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Infrared Spectra and the Chain Organization of Erythrocyte Membranes

Cited by 71 publications

References 12 publications

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Current Models for the Structure of Biological Membranes

Vascular graft healing. II. FTIR analysis of polyester graft samples from implanted bi-grafts

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