Infrared Spectrophotometry as a Means for Identification of Bacteria

“…Since the original work of Stevenson & Bolduan (1952) a number of reports have been published on the application of infra-red spectroscopy to the differentiation of bacteria and other micro-organisms. A paper by O'Connor, McCall & Du Pr6 (1957) gives references to most of the relevant work published in the U.S.A., and to this should be added the papers by Thomas & Greenstreet (1954), Lembke & Kaufmann (1954), Greenstreet & Norris (1957) and Rideal & Adams (1957).…”

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confidence: 99%

The Infra-Red Absorption Spectra of Lactobacilli

Goulden

Sharpe

1958

Journal of General Microbiology

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SUMMARY:The infra-red absorption spectra of 76 strains of lactobacilli representing 11 different species were examined. With only a few exceptions, the spectra could be grouped into 5 distinct spectral types, each corresponding to a Lactobacillus species or a group of related species. Fkom a study of acetic acid extracts, it has been found possible to relate spectral differences to the relative concentrations of nucleic acids and carboxylate groups in the different organisms.

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“…In the 1950s, similar approaches were applied for the identification of different species and strains of bacteria [18], to study living muscle cells from insects and animals [19], and for the comparison of spectral features of normal and neoplastic tissues and the chemical constituents (e.g., nucleic acids, carbohydrates, fats and proteins) [20]. Because the measurement process was slow and data analysis was time-consuming, infrared spectral microscopy did not become a widely used tool for studying cellular or tissue systems until the 1990s as a result of three technological breakthroughs: (i) the application of the fast Fourier transform algorithm, (ii) the availability of inexpensive, fast digital computers which enabled the replacement of dispersive spectrometers with FTIR interferometers, and (iii) the introduction of fast-response, high-sensitivity photoconductive single-element mercury cadmium telluride (MCT) detectors.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic approaches to synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy of living biosystems

Loutherback¹,

Birarda²,

Chen³

et al. 2016

PPL

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A long-standing desire in biological and biomedical sciences is to be able to probe cellular chemistry as biological processes are happening inside living cells. Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy is a label-free and nondestructive analytical technique that can provide spatiotemporal distributions and relative abundances of biomolecules of a specimen by their characteristic vibrational modes. Despite great progress in recent years, SR-FTIR imaging of living biological systems remains challenging because of the demanding requirements on environmental control and strong infrared absorption of water. To meet this challenge, microfluidic devices have emerged as a method to control the water thickness while providing a hospitable environment to measure cellular processes and responses over many hours or days. This paper will provide an overview of microfluidic device development for SR-FTIR imaging of living biological systems, provide contrast between the various techniques including closed and open-channel designs, and discuss future directions of development within this area. Even as the fundamental science and technological demonstrations develop, other ongoing issues must be addressed; for example, choosing applications whose experimental requirements closely match device capabilities, and developing strategies to efficiently complete the cycle of development. These will require imagination, ingenuity and collaboration.

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Infrared Spectrophotometry as a Means for Identification of Bacteria

Cited by 74 publications

References 7 publications

Infrared Absorption Spectra of Whole Cells of Nocardia and Related Organisms

Infrared Absorption Spectra of Whole Cells of Nocardia and Related Organisms

The Infra-Red Absorption Spectra of Lactobacilli

Microfluidic approaches to synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy of living biosystems

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