Infrared spectroscopic study of diabetic platelets

Liu, Kan‐Zhi; Bose, Ratna; Mantsch, Henry H.

doi:10.1016/s0924-2031(01)00163-1

Cited by 64 publications

(62 citation statements)

References 17 publications

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“…Hence, the intensity of the olefinic band was increased due to the accumulation of end products of lipid peroxidation. Our results are in accordance with the study of Liu et al [71]. The average band intensity reverted to near normal when the ethanol-fed animals were treated with the H. indicus extract, which may be due to the antioxidant properties of the extract.…”

Section: Discussionsupporting

confidence: 93%

Hemidesmus indicus Protects against ethanol-induced liver toxicity

Saravanan

Nalini

2008

Cellular and Molecular Biology Letters

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Alcoholic liver disease (ALD) is one of the most common diseases in modern society. A large number of studies are in progress aiming to identify natural substances that would be effective in reducing the severity of ALD. Although there are currently a number of drugs on the market, their long-term use can have numerous side effects. Hemidesmus indicus is an indigenous Ayurvedic medicinal plant used in soft drinks in India. In this study, we examined the effects of its ethanolic root extract on experimental liver damage in order to evaluate its hepatoprotective effects against hepatotoxicity induced in rats by ethanol at a dosage of 5 g/kg body weight for 60 days. The H. indicus root extract was given at a dose of 500 mg/kg body weight for the last 30 days of the experiment. The animals were monitored for food intake and weight gain. The liver was analysed for the degree of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) and antioxidant status using the activities of glutathione-depedendant enzymes. The degree of liver damage was analysed using serum marker enzyme activities, the total protein, albumin, globulin, ceruloplasmin and liver glycogen contents, and the A/G ratio. The Fourier transform infrared spectra (FT-IR) of the liver tissues were recorded in the region of 4000-400 cm -1 . The ethanol-fed rats showed significantly elevated liver marker enzyme activities, lipid peroxidation levels and reduced antioxidant levels as compared to the control rats. Oral administration of H. indicus for the latter 30 days resulted in an increased food intake and weight gain, decreased TBARS levels, near normal levels of glutathione-dependent enzymes, increased total protein, albumin, globulin and liver glycogen contents, an increased A/G ratio, and decreased liver marker enzyme activities and ceruloplasmin levels. The relative intensity of the liver FT-IR bands for the experimental groups were found to be altered significantly (p < 0.05) compared to the control samples. For the group that had H. indicus co-administered with ethanol, the intensity of the bands was near normal. Moreover, the results of the FT-IR study correlated with our biochemical results.

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Section: Discussionsupporting

confidence: 93%

Hemidesmus indicus Protects against ethanol-induced liver toxicity

Saravanan

Nalini

2008

Cellular and Molecular Biology Letters

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“…Rat liver microsomal membranes, kidney brush border membranes, EDL and SOL skeletal muscles were chosen for the study. Spectral parameters, such as shifts in peak positions, changes in bandwidths, intensities, and band area values of the infrared bands can be used to obtain valuable structural and functional information about the system of interest, which may have diagnostic value [20,23,26,27,32,39]. Figures 1A and 2A shows the representative spectra of control and diabetic liver microsomal and Figure 1 (A) The representative spectra of control and diabetic liver microsomal membranes in the 4000-1000 cm À1 region.…”

Section: Resultsmentioning

confidence: 99%

“…FT-IR spectroscopy together with microscopy has been used previously to study diabetes-induced alterations in tissues and membranes in animal models [27,[32][33][34][35] and human blood [23]. Since blood sugar regulation is a serious problem in DM, recently a method was developed using ATR-FT-IR spectroscopy together with partial least-squares regression analysis for fast and reliable determination of the glucose level in the blood of diabetes patients.…”

Section: Biophotonicsmentioning

confidence: 99%

FT‐IR spectroscopy in diagnosis of diabetes in rat animal model

Severcan

Bozkurt

Gurbanov

et al. 2010

Journal of Biophotonics

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In recent years, Fourier Transform Infrared (FT-IR) spectroscopy has had an increasingly important role in the field of pathology and diagnosis of disease states. In the current study, FT-IR spectroscopy together with cluster analysis were used as a diagnostic tool in the discrimination of diabetic samples from control ones in rat kidney plasma membrane apical sides (brush-border membranes), liver microsomal membranes and Extensor digitorum longus (EDL) and Soleus (SOL) skeletal muscle tissues. A variety of alterations in the spectral parameters, such as frequency and signal intensity/area was observed in diabetic tissues and membranes compared to the control samples. Based on these spectral variations, using cluster analysis successful differentiation between diabetic and control groups was obtained in different spectral regions. The results of this current study further revealed the power and sensitivity of FT-IR spectroscopy in precise and automated diagnosis of diabetes.

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“…The bands arise from lipids, phospholipids and membranes. The intensity of symmetric and asymmetric stretching vibrations of CH 2 and CH 3 reflect lipid hyperoxidation (Liu et al, 2002). The increase in the intensity of the bands in coronary artery shows that the environment is less lipophilic due to fragmentation of the lipoproteins and accumulation of free cholesterol and cholesterol esters in the atheromatous core, as a result membrane fluidity changes significantly (Anastassopoulou and Theophanides, 1990).…”

Section: Analysis and Discussion Of Ft-ir Spectramentioning

confidence: 99%

FT-IR Spectroscopy in Medicine

Dritsa¹

2012

Infrared Spectroscopy - Life and Biomedical Sciences

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Infrared spectroscopic study of diabetic platelets

Cited by 64 publications

References 17 publications

Hemidesmus indicus Protects against ethanol-induced liver toxicity

Hemidesmus indicus Protects against ethanol-induced liver toxicity

FT‐IR spectroscopy in diagnosis of diabetes in rat animal model

FT-IR Spectroscopy in Medicine

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