Infrared spectroscopy as a tool for investigations of DNA structure and DNA - ligand interactions

Pohle, Walter; Fritzsche, H.

doi:10.1016/0022-2860(90)80079-y

Cited by 7 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Low water content and high concentrations of solute make such samples ideal for IR spectroscopy (Fritzsche et al 1976(Fritzsche et al , 1984. Pohle & Fritzsche (1990) have surveyed the potential of infra-red spectroscopy to determine conformations of nucleic acids, including the use of phosphate vibrations and the influence of drug molecules. Fritzsche (1990) has shown that the B to A transition, driven by the reduced water activity, is affected by the interaction of DNA with intercalating as well as nonintercalating antitumour drugs.…”

Section: Films Of Pure Substratementioning

confidence: 99%

Linear dichroism spectroscopy of nucleic acids

Nordén

Kubista

Kurucsev

1992

Quart. Rev. Biophys.

360

376

View full text Add to dashboard Cite

This review will consider solution studies of structure and interactions of DNA and DNA complexes using linear dichroism spectroscopy, with emphasis on the technique of orientation by flow. The theoretical and experimental background to be given may serve, in addition, as a general introduction into the state of the art of linear dichroism spectroscopy, particularly as it is applied to biophysical problems.

show abstract

Section: Films Of Pure Substratementioning

confidence: 99%

Linear dichroism spectroscopy of nucleic acids

Nordén

Kubista

Kurucsev

1992

Quart. Rev. Biophys.

360

376

View full text Add to dashboard Cite

show abstract

“…Infrared (IR) spectroscopy is a well-known tool to characterize DNA bases, morphological changes in DNA, and binding of drugs and proteins to DNA in solution assays. − In this paper we investigate the possibilities for label free detection of hybridization using the chemical fingerprint of DNA in the IR frequency range. , Investigation of nanometer sized DNA patches with IR spectroscopy is an experimental challenge, because the inherent diffraction limit is in the range of 5−10 μm for IR wavelength. Scattering scanning near-field infrared microscopy (s-SNIM) is a scanning probe microscopy (SPM) based technique which overcomes the diffraction limit while maintaining its spectroscopic capabilities.…”

Section: Introductionmentioning

confidence: 99%

Detection of Hybridization on Nanografted Oligonucleotides Using Scanning Near-Field Infrared Microscopy

et al. 2009

View full text Add to dashboard Cite

We demonstrate that scattering scanning near-field infrared microscopy (s-SNIM) is a label free analytical method allowing hybridization detection of nanografted DNA patches on a sub-µM scale. On the basis of their distinct dielectric properties in the IR, we can distinguish between single stranded and double stranded DNA. The sensitivity of s-SNIM is found to be increased by 7 orders of magnitude compared to conventional FTIR spectroscopy due to the tip enhanced interaction with the substrate.

show abstract

Fourier transform infrared spectroscopy as a probe for the study of the hydration of lipid self‐assemblies. I. Methodology and general phenomena

et al. 1998

View full text Add to dashboard Cite

An algorithm for the study of the gradual hydration of phospholipid assemblies by means of Fourier transform infrared (FTIR) spectroscopy is presented. A complete series of diacyl phosphatidylcholines (PCs) including all possible analogues with palmitoyl and oleoyl residues, namely DPPC, DOPC, POPC, and OPPC, was investigated at room temperature. The lipid samples were prepared as cast films probably consisting of aligned multilamellar bilayers. The range of water activities studied in these films was regulated by adsorption via the gas phase corresponding to relative humidities of between 0 and 100%. Analyses of the IR‐spectroscopic data have concentrated mainly on determining the amounts of water incorporated by each lipid as well as the hydration‐induced response observed for some absorption bands of the different lipids. The water uptake at high relative humidity (RH) increases with the portion of unsaturated acyl chains in the molecular structure of the PCs. Isothermal phase transitions triggered lyotropically have been detected in demonstrating the occurrence of the main transition in POPC and OPPC films at room temperature. Moreover, it appears that both lamellar phases, the gel as well as the liquid‐crystalline phase, are not uniform. They seem to comprise an amazingly large span of order/disorder states of the lipid chains generally depending on the degree of hydration. As exemplified by the significant variation in the onset of wavenumber shifts for the PO 2− and C=O stretching‐vibration modes, obtained as a function of hydration, a sequence of attachment to polar lipid binding sites by water molecules was established for DPPC. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 267–280, 1998

show abstract

Infrared spectroscopy as a tool for investigations of DNA structure and DNA - ligand interactions

Cited by 7 publications

References 22 publications

Linear dichroism spectroscopy of nucleic acids

Linear dichroism spectroscopy of nucleic acids

Detection of Hybridization on Nanografted Oligonucleotides Using Scanning Near-Field Infrared Microscopy

Fourier transform infrared spectroscopy as a probe for the study of the hydration of lipid self‐assemblies. I. Methodology and general phenomena

Contact Info

Product

Resources

About