Olive oil, especially extra virgin olive oil, gained popularity recently due to its beneficial
effects toward human health. Olive oil contained high amounts of monounsaturated fatty
acids, especially oleic acid (C18:1) and some minor components such as tyrosol and
hydroxytyrosol which are important to human health. Olive oils have been reported to
have antioxidant in vitro and in vivo. This article reviewed some physico-chemical
properties and antioxidant activities of olive oil either in vitro or in vivo. Olive oil was
evaluated in vitro using radical scavenging activities, ferric reducing antioxidant power
(FRAP), metal chelating power, beta-carotene bleaching, linoleic acid-ferric thiocyanate
method. In vivo, the antioxidant activities of olive oil were evaluated using glutathione Stransferase, catalase, and superoxide dismutase. Phenolics compounds present in olive oil
contribute to antioxidant activities, therefore, olive oil is potential to be used as a food
supplement.