Infrared Spectroscopy in Aqueous Solutions: Capabilities and Challenges

Fomina, Polina; Проскурнин, М. А.; Mizaikoff, Boris; Volkov, Dmitry S.

doi:10.1080/10408347.2022.2041390

Cited by 20 publications

(14 citation statements)

References 137 publications

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“…All the main FTIR bands coincide with those previously known for graphene oxide. To obtain the finest FTIR spectra from aqueous EGO dispersions, we placed samples into ATR diamond crystal at 50 °C to evaporate water, which led to the formation of a thin film of the sample and better adhesion to the crystal surface in comparison with standard application and pellet pressing, as previously discussed in [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Green and Sustainable Ultrasound-Assisted Anodic Electrochemical Preparation of Graphene Oxide Dispersions and Their Antioxidant Properties

et al. 2023

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A fast method for preparing aqueous graphene oxide (GO) dispersions by electrochemical oxidation of a graphite anode without preliminary intercalation with oxidizing agents is proposed. Ultrasonic probing was used in the modulation mode of ultrasonic waves (work/rest) for more efficient graphite oxidation–exfoliation. It is shown that the 4/2 s mode of ultrasonic modulation is the most effective due to the probe material’s low corrosion while maintaining the optimum synthesis temperature not exceeding 30–35 °C and achieving the best characteristics of the resulting product. Three cases of anodic oxidation of graphite to obtain graphene oxide were considered: (1) a combined cathode–anode compartment, (2) a split cathode–anode salt-bridged compartment, and (3) separated anode compartment with a 3.5 kDa dialysis membrane. It was determined that the approach to synthesis with a divided cathode–anode compartment makes it possible to obtain GO sheets with fewer defects compared to chemical methods or methods with a combined cathode–anode compartment and makes it possible to control the oxidation degree of the material (C:O ratio) by varying the current density. The prepared samples showed good stability for more than six months. The spectral and morphological characteristics were studied. Using chemiluminometry in the luminol/Co(II)/H2O2 system, the antioxidant properties concerning three key reactive oxygen species (H2O2, superoxide anion radical, and hydroxyl radical) were demonstrated. It was also shown that the prepared GO dispersions do not induce lipid and phospholipid peroxidation.

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Section: Resultsmentioning

confidence: 99%

Green and Sustainable Ultrasound-Assisted Anodic Electrochemical Preparation of Graphene Oxide Dispersions and Their Antioxidant Properties

et al. 2023

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“…87 Additionally, as all biomolecular condensates consist largely of water and are in liquid solutions, the absorbance spectrum of water obscures chemical insights between areas of high absorption within the spectrum (as most of the excitation beam is absorbed by the liquid). 88 Future Perspectives. Spectroscopic tools are a great complement to probe the chemical makeup of biomolecular condensates.…”

Section: Part 1: Imaging-based Techniquesmentioning

confidence: 99%

“…Additionally, the spot size of the excitation beam is diffraction limited, typically probing a cross sectional volume of ∼0.5–1 cubic micrometer . Additionally, as all biomolecular condensates consist largely of water and are in liquid solutions, the absorbance spectrum of water obscures chemical insights between areas of high absorption within the spectrum (as most of the excitation beam is absorbed by the liquid) …”

Section: Part 1: Imaging-based Techniquesmentioning

confidence: 99%

Label-Free Techniques for Probing Biomolecular Condensates

Ibrahim,

Naidu,

Miljkovic

et al. 2024

ACS Nano

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Biomolecular condensates play important roles in a wide array of fundamental biological processes, such as cellular compartmentalization, cellular regulation, and other biochemical reactions. Since their discovery and first observations, an extensive and expansive library of tools has been developed to investigate various aspects and properties, encompassing structural and compositional information, material properties, and their evolution throughout the life cycle from formation to eventual dissolution. This Review presents an overview of the expanded set of tools and methods that researchers use to probe the properties of biomolecular condensates across diverse scales of length, concentration, stiffness, and time. In particular, we review recent years’ exciting development of label-free techniques and methodologies. We broadly organize the set of tools into 3 categories: (1) imaging-based techniques, such as transmitted-light microscopy (TLM) and Brillouin microscopy (BM), (2) force spectroscopy techniques, such as atomic force microscopy (AFM) and the optical tweezer (OT), and (3) microfluidic platforms and emerging technologies. We point out the tools’ key opportunities, challenges, and future perspectives and analyze their correlative potential as well as compatibility with other techniques. Additionally, we review emerging techniques, namely, differential dynamic microscopy (DDM) and interferometric scattering microscopy (iSCAT), that have huge potential for future applications in studying biomolecular condensates. Finally, we highlight how some of these techniques can be translated for diagnostics and therapy purposes. We hope this Review serves as a useful guide for new researchers in this field and aids in advancing the development of new biophysical tools to study biomolecular condensates.

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“…This technique can analyse complex mixtures simultaneously, revealing adequate qualitative and quantitative information. 11 With regards to aqueous solutions, FTIR has applications in several fields, such as diabetes monitoring, 12 food additives, 13 allergens 14 and bio-hybrid fuel cells. 15 Compounds of interest resulting from conversion processes have also been analysed, such as the sugar content in enzymatic hydrolysis of alkali-pretreated biomasses, 16 the quantification of glucose in aqueous solutions, 17 and quantification of aqueous phases (bio-crudes) derived from HTL.…”

Section: Introductionmentioning

confidence: 99%

“…Absorption peaks of chemical bonds under aqueous mid-infrared radiation are broad, spreading across several wavelengths. 11 Solutions such as feature engineering using statistical values has shown to capture the interconnection of movements by depth sensors. 27 Similarly, hand-crafted statistical features could also be applicable to the vibrational intensity across wavelengths and amplify the response.…”

Section: Introductionmentioning

confidence: 99%