Infrared Spectroscopy of Biofluids in Clinical Chemistry and Medical Diagnostics

Shaw, R. Anthony; Low-Ying, Sarah; Man, Angela; Liu, Kan‐Zhi; Mansfield, Colin D.; Rileg, Christopher B.; Vijarnsorn, Mouchanoh

doi:10.1002/9780470283172.ch4

Cited by 12 publications

(18 citation statements)

References 46 publications

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“…With the global disease burden set to rise, a more rapid, non-invasive, label-free, nondestructive, automatic and cost effective diagnostic technique like vibrational spectroscopy would revolutionise the clinical environment. Its utility as a biofluid diagnostic tool is heavily reliant on the principle that cellular and tissue dysfunction or irregularities affect the biochemical make-up of biofluids, manifesting as protein, carbohydrate, lipid, and nucleic acid subtle differences 16 .…”

Section: These Limitations Can Be Partly Circumvented With Other Ramamentioning

confidence: 99%

Developing and understanding biofluid vibrational spectroscopy: a critical review

et al. 2016

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Vibrational spectroscopy can provide rapid, label-free, and objective analysis for the clinical domain. Spectroscopic analysis of biofluids such as blood components (e.g. serum and plasma) and others in the proximity of the diseased tissue or cell (e.g. bile, urine, and sputum) offers non-invasive diagnostic/monitoring possibilities for future healthcare that are capable of rapid diagnosis of diseases via specific spectral markers or signatures. Biofluids offer an ideal diagnostic medium due to their ease and low cost of collection and daily use in clinical biology. Due to the low risk and invasiveness of their collection they are widely welcomed by patients as a diagnostic medium. This review underscores recent research within the field of biofluid spectroscopy and its use in myriad pathologies such as cancer and infectious diseases. It highlights current progresses, advents, and pitfalls within the field and discusses future spectroscopic clinical potentials for diagnostics. The requirements and issues surrounding clinical translation are also considered.

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Section: These Limitations Can Be Partly Circumvented With Other Ramamentioning

confidence: 99%

Developing and understanding biofluid vibrational spectroscopy: a critical review

et al. 2016

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“…Among the emerging applications of infrared (IR) spectroscopy are powerful quantitative and qualitative techniques for the diagnostic characterization of biological molecules in fluids and tissues. 13–16 These complex samples produce IR spectra composed of overlapping absorption bands that reflect the nature and concentration of their constituent molecular species. 14–19 The IR absorption patterns for biological samples may be viewed as biochemical signatures that not only provide the basis to quantify the diagnostically relevant various serum, urine, and other biological fluid analytes, but may also be directly correlated with the presence or absence of diseases.…”

Section: Introductionmentioning

confidence: 99%

Immunoglobulin G Measurement in Blood Plasma Using Infrared Spectroscopy

Hou

McClure

Shaw³

et al. 2014

Appl Spectrosc

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A rapid, simple, and inexpensive method to measure the immunoglobulin G (IgG) concentrations in blood samples in human and veterinary medicine is highly desired. Infrared spectroscopy (coupled with chemometric manipulation of spectral data) has the advantages of simple sample preparation, rapid implementation of analysis, and low cost. Here a method that exploits infrared spectroscopy as the basis to measure IgG concentration in animal plasma samples is reported, with radial immunodiffusion (RID) used as the reference test method for partial least squares (PLS) calibration model development. Smoothed non-derivative and the second-order derivative spectra were used to develop calibration models. Various additional spectral preprocessing steps were evaluated to optimize the calibration models, and the possible benefits of using an internal standard (potassium thiocyanate [KSCN]) were investigated. Monte Carlo cross-validation was used to determine the optimal number of PLS factors, and an independent prediction set was used to test the predictive performances of provisional models. The effects of various preprocessing options (spectral smoothing, derivation, normalization, region selection, mean-centering, and standard deviation scaling) on quantification accuracy were investigated. The root mean squared error of prediction (RMSEP) for different combinations of spectra preprocessing steps was 394 ± 36 mg/dL for the non-derivative spectra and 427 ± 101 mg/dL for the second-order derivative spectra. Immunoglobulin G concentrations produced by the optimized PLS model for the non-derivative spectra (RMSEP = 352 mg/dL) were found to be stable with respect to different splits of the samples among the calibration, validation, and prediction sets. The precision of the Fourier transform infrared (FT-IR) method is found to be slightly superior to that of the RID method. The results of this work indicate that infrared spectroscopy is a promising technique for economically and rapidly determining the IgG concentrations of plasma and plasma-derived samples.

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“…Since most of the clinical analytical instruments are accurate for both serum and plasma, these two terms are used erroneously interchangeably in most clinical tests [7]. Notably, many studies that have been reported to be carried out in serum were in fact carried out in plasma Although ion exchange chromatography is a quick method to separate proteins, this method has to be tailored for a specific protein depending on its charge and cannot be used as a 'onefor-all' separation kit for all the proteins.…”

Section: Measurement Of Plasma Vs Serummentioning

confidence: 99%

“…Vibrational spectroscopic techniques, both Raman and Infrared (IR) absorption, have been extensively explored over the last two decades for obtaining the biochemical composition of bodily fluids in the field of biomedical analysis [1][2][3][4][5][6][7][8][9][10]. The sensitivity to detect subtle changes in the biochemical composition and ability to detect the presence of specific biomarkers or drugs makes vibrational spectroscopy an ideal tool for the early diagnosis of various pathologies [11][12][13][14] and therapeutic drug monitoring [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

2020

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There is compelling evidence in the literature to support the application of Raman spectroscopy for analysis of bodily fluids in their native liquid state. Naturally, the strategies described in the literature for Raman spectroscopic analysis of liquid samples have advantages and disadvantages. Herein, recent advances in the analysis of plasma/serum in the liquid state are reviewed. The potential advantages of Raman analysis in the liquid form over the commonly employed infrared absorption analysis in the dried droplet form are initially highlighted.Improvements in measurement protocols based on inverted microscopic geometries, clinically adaptable substrates, data preprocessing and analysis, and applications for routine monitoring of patient health as well as therapeutic administration are reviewed. These advances suggest that clinical translation of Raman spectroscopy for rapid biochemical analysis can be a reality.In the future, this method will prove to be highly beneficial to clinicians for rapid screening and monitoring of analytes and drugs in the biological fluids, and to the patients themselves, enabling early treatment, before the disease becomes symptomatic, allowing early recovery.

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Infrared Spectroscopy of Biofluids in Clinical Chemistry and Medical Diagnostics

Cited by 12 publications

References 46 publications

Developing and understanding biofluid vibrational spectroscopy: a critical review

Developing and understanding biofluid vibrational spectroscopy: a critical review

Immunoglobulin G Measurement in Blood Plasma Using Infrared Spectroscopy

Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

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