Infrared synchrotron radiation from electron storage rings

Duncan, William; Williams, Gwyn

doi:10.1364/ao.22.002914

Cited by 223 publications

(122 citation statements)

References 16 publications

Supporting

Mentioning

118

Contrasting

Unclassified

Order By: Relevance

“…These problems are overcome by synchrotron IR light by virtue of its brightness, which is 100-1000 times greater than that from a conventional source (13). Individual biological cells range from 5-30 μm in diameter, too small for probing by conventional IR sources.…”

Section: Discussionmentioning

confidence: 99%

Fourier transform infrared spectromicroscopy and hierarchical cluster analysis of human meningiomas

Ali

Lu²,

Christensen³

et al. 2008

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Limitations of conventional light microscopy in pathological diagnosis of brain tumors include subjective bias in interpretation and discordance of nomenclature. A study using mid-infrared (IR) spectromicroscopy was undertaken to determine whether meningiomas, a group of brain tumors prone to recurrence, could be identified by the unique spectral 'fingerprints' of their chemical composition. Paired, thin (5-μm) cryosections of snap-frozen human meningioma tumor samples removed at elective surgery were mounted on glass (hematoxylin and eosin-stained tissue section) and infrared (unstained tissue section) reflectance slides, respectively. Concordance of the tumor-bearing areas identified in the stained section by a pathologist with the unstained IR tissue section was ensured using a novel digital grid and tumor-mapping system developed in our laboratory. Compared with the normal control, tumor samples from four meningioma patients revealed a marked decrease in bands associated with unsaturated fatty acids, particularly in the bands at 3010, 2920, 2850, and 1735 cm -1 . Spectral datasets were subjected to hierarchical cluster analyses (HCA) using Ward's algorithm for comparison and grouping of similar data groups, and were converted into color-coded digital maps for matching spectra with their respective clusters. False color images of 5 and 6 clusters obtained by HCA identified dominant clusters corresponding to tumor tissue. Corroboration of these findings in a larger number of meningiomas may allow for more precise identification of these and other types of brain tumors.

show abstract

Section: Discussionmentioning

confidence: 99%

Fourier transform infrared spectromicroscopy and hierarchical cluster analysis of human meningiomas

Ali

Lu²,

Christensen³

et al. 2008

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…The highly collimated nature of SR means that more intensity can be focused through a small aperture, which is ideal for spectromicroscopy and other applications where it is desired to probe a sample with high spatial resolution. Infrared beamlines and the associated apparatus for such purposes have developed rapidly during the past 20 years [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…new or unexpected species) of importance for investigating the chemistry of stellar formation. Xu and Lees have also recorded the spectrum of the related molecule methyl mercaptan, CH 3 by McKellar and Billinghurst [70].…”

mentioning

confidence: 99%

High-resolution infrared spectroscopy with synchrotron sources

McKellar

2010

Journal of Molecular Spectroscopy

View full text Add to dashboard Cite

“…Consequently, most designers choose to maximize the throughput of the system rather than achieve the best possible spatial resolution at the diffraction limit. Synchrotron sources, which are 100-1000 times brighter than a typical blackbody source [1][2][3][4][5][6][7][8] , have made diffraction-limited spatial resolutions possible. Here, we discuss a simple modification to commercial infrared microscopes that allows the user to take full advantage of the spatial resolution achievable by synchrotron infrared radiation.…”

Section: Introductionmentioning

confidence: 99%

Improved Spatial Resolution For Reflection Mode Infrared Spectromicroscopy

Bechtel¹,

Martin²,

May³

et al. 2010

AIP Conference Proceedings

View full text Add to dashboard Cite

Abstract. Standard commercial infrared microscopes operating in reflection mode use a mirror to direct the reflected light from the sample to the detector. This mirror blocks about half of the incident light, however, and thus degrades the spatial resolution by reducing the numerical aperture of the objective. Here, we replace the mirror with a 50% beamsplitter to allow full illumination of the objective and retain a way to direct the reflected light to the detector. The improved spatial resolution is demonstrated using a microscope coupled to a synchrotron source.

show abstract

Infrared synchrotron radiation from electron storage rings

Cited by 223 publications

References 16 publications

Fourier transform infrared spectromicroscopy and hierarchical cluster analysis of human meningiomas

Fourier transform infrared spectromicroscopy and hierarchical cluster analysis of human meningiomas

High-resolution infrared spectroscopy with synchrotron sources

Improved Spatial Resolution For Reflection Mode Infrared Spectromicroscopy

Contact Info

Product

Resources

About