Infrared video microscopy—an efficient-method for the routine investigation of opaque organic-walled microfossils

Brocke, Rainer; Wilde, Volker

doi:10.1007/bf02668109

Cited by 8 publications

(3 citation statements)

References 14 publications

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“…Color photographs and Z-stack images were performed under a Nikon SMZ25 microscope, using Nikon SHR Plan Apo 0.5x and SHR Plan Apo 2x objectives with a Nikon DS-Ri2 camera and NIS-Element software (version 4.51.00; ) and a digital camera attached to an Olympus BX51 compound microscope. Black and white infrared reflected photomicrographs were taken with a Nikon Eclipse ME600D (see Brocke & Wilde, 2001 for precise technical information). Photographs were Z-stacked using the NIS-Element software.…”

Section: Methodsmentioning

confidence: 99%

A new genus of dance fly (Diptera: Empidoidea: Hybotidae) from Cretaceous Spanish ambers and introduction to the fossiliferous amber outcrop of La Hoya (Castellón Province, Spain)

Solórzano-Kraemer

Sinclair

Arillo

et al. 2023

PeerJ

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Hybotidae fly species, also known as dance flies, in Cretaceous ambers have been described from Lebanon, France, Myanmar, Russia, and Canada. Here we describe Grimaldipeza coelica gen. et sp. n., and recognize another two un-named species, in Spanish amber from the middle Albian El Soplao and lower Cenomanian La Hoya outcrops. The fore tibial gland is present in the new genus, which is characteristic of the family Hybotidae. We compare Grimaldipeza coelica gen. et sp. n. with the holotypes of Trichinites cretaceus Hennig, 1970 and Ecommocydromia difficilis Schlüter, 1978, and clarify some morphological details present in the latter two species. Further taxonomic placement beyond family of the here described new genus was not possible and remains incertae sedis within Hybotidae until extant subfamilies are better defined. We provide new paleoecological data of the hybotids, together with paleogeographical and life paleoenvironmental notes. A table with the known Cretaceous Hybotidae is provided. Furthermore, the La Hoya amber-bearing outcrop is described in detail, filling the information gap for this deposit.

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Section: Methodsmentioning

confidence: 99%

A new genus of dance fly (Diptera: Empidoidea: Hybotidae) from Cretaceous Spanish ambers and introduction to the fossiliferous amber outcrop of La Hoya (Castellón Province, Spain)

Solórzano-Kraemer

Sinclair

Arillo

et al. 2023

PeerJ

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“…Drawings were made using a Leica drawing tube attached to a Leica MZ12 stereomicroscope (Senckenberg Forschungsinstitut und Naturmuseum, Frankfurt, Germany). Infrared reflected photomicrographs were taken with a Nikon Eclipse ME600D at the Senckenberg Forschungsinstitut und Naturmuseum (see Brocke & Wilde (2001) for precise technical information). Original photographs were z-stacked using the Photoshop CS3 software.…”

Section: Methodsmentioning

confidence: 99%

The semi-aquatic pondweed bugs of a Cretaceous swamp

Sánchez‐García

Nel

Arillo

et al. 2017

PeerJ

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Pondweed bugs (Hemiptera: Mesoveliidae), considered a sister group to all other Gerromorpha, are exceedingly rare as fossils. Therefore, each new discovery of a fossil mesoveliid is of high interest, giving new insight into their early evolutionary history and diversity and enabling the testing of their proposed relationships. Here, we report the discovery of new mesoveliid material from Spanish Lower Cretaceous (Albian) amber, which is the first such find in Spanish amber. To date, fossil records of this family only include one species from French Kimmeridgian as compression fossils, two species in French amber (Albian-Cenomanian boundary), and one in Dominican amber (Miocene). The discovery of two males and one female described and figured as Glaesivelia pulcherrima Sánchez-García & Solórzano Kraemer gen. et sp. n., and a single female described and figured as Iberovelia quisquilia Sánchez-García & Nel, gen. et sp. n., reveals novel combinations of traits related to some genera currently in the subfamily Mesoveliinae. Brief comments about challenges facing the study of fossil mesoveliids are provided, showing the necessity for a revision of the existing phylogenetic hypotheses. Some of the specimens were studied using infrared microscopy, a promising alternative to the systematic study of organisms preserved in amber that cannot be clearly visualised. The new taxa significantly expand the fossil record of the family and shed new light on its palaeoecology. The fossils indicate that Mesoveliidae were certainly diverse by the Cretaceous and that numerous tiny cryptic species living in humid terrestrial to marginal aquatic habitats remain to be discovered. Furthermore, the finding of several specimens as syninclusions suggests aggregative behaviour, thereby representing the earliest documented evidence of such ethology.

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“…Characteristic representatives of the recorded taxa are illustrated on Plates 1-3. They were analysed and documented using a Nikon Eclipse 90i microscope, equipped with an infrared video system (specified in Brocke & Wilde, 2001) for studying dark-coloured to nearly opaque palynomorphs (e.g. Pl.…”

Section: Methodsmentioning

confidence: 99%

A fungal proliferation near the probable Oligocene/Miocene boundary, Nukhul Formation, Gulf of Suez, Egypt

Atfy

Brocke

Uhl

2013

J. Micropalaeontol.

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Abstract. Moderately to well-preserved palynomorph assemblages were recorded from thirty samples of the Nukhul Formation (GH 404-2A Well), southern Gulf of Suez, Egypt. The taxa are dominated by highly diverse fungi, freshwater algae (e.g. Botryococcus, Pediastrum) beside a sparse record of spores and pollen. Marine palynomorphs, such as dinoflagellate cysts (dinocysts), are very rare. The stratigraphy and age of the Nukhul Formation is highly debated due to lack of diagnostic fossils (e.g. foraminifera, nannoplankton). It has been referred mostly to the Early Miocene; however, some recent publications interpret it as being of latest Oligocene–Early Miocene age. A prominent fungal proliferation composed of diverse and moderately well-preserved fungal spores, fungal fragments, fructifications and hyphae is recorded. This fungi-rich interval occurs mainly from 11370 to 11430 ft in the GH 404-2A Well. Such an observation has not been noted previously within the Nukhul Formation or its stratigraphic equivalents in Egypt. This putative ‘eco-event’ is probably associated with the well-known eustatic sea-level fall in the latest Chattian to early Aquitanian or at the Oligocene/Miocene boundary (OMB). It also seems possible that it may represent a more local event related to the rifting of the Gulf of Suez during this period. The high diversity of fossil fungi is interpreted herein as an indication of an episodic prevalence of humid climate at the end of a regressive phase, as also indicated by a lithological change near the top of the Shoab Ali Member of the Nukhul Formation. In addition, the co-occurrence of freshwater algae, mainly Botryococcus and Pediastrum, together with some aquatic fungal genera, such as Involutisporonites, Paragrantisporites, Quilonia, Striadiporites and Reduviasporonites, suggests the temporary existence of shallow, pond- or lake-like aquatic habitats, possibly related to tectonic activity.

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Infrared video microscopy—an efficient-method for the routine investigation of opaque organic-walled microfossils

Cited by 8 publications

References 14 publications

A new genus of dance fly (Diptera: Empidoidea: Hybotidae) from Cretaceous Spanish ambers and introduction to the fossiliferous amber outcrop of La Hoya (Castellón Province, Spain)

A new genus of dance fly (Diptera: Empidoidea: Hybotidae) from Cretaceous Spanish ambers and introduction to the fossiliferous amber outcrop of La Hoya (Castellón Province, Spain)

The semi-aquatic pondweed bugs of a Cretaceous swamp

A fungal proliferation near the probable Oligocene/Miocene boundary, Nukhul Formation, Gulf of Suez, Egypt

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