Infraspecific variation of some brown Parmeliae (in Poland) – a comparison of ITS rDNA and non-molecular characters

Abstract

Infraspecific variation of the ITS rDNA region of some brown Parmeliae occurring in Poland is studied and compared with non-molecular characters. Haplotype networks are used to illustrate the variability within the species. Both newly-produced sequences from Central Europe and from all over the world, downloaded from the GenBank, are used. The number of haplotypes found for each taxon ranged from five in Melanelia stygia to 12 in Melanelia hepatizon and Montanelia disjuncta; however, their numbers correlate with the number of specimens tested. New haplotypes for Melanelia agnata, M. hepatizon and Cetraria commixta are found. Based on our 169-sample dataset, we could not infer any geographical correlation, either locally or world-wide. Many of the analysed haplotypes were widely distributed and the same haplotype was often shared between temperate and polar populations. A comparison of molecular, morphological, anatomical and chemical characters also shows no correlation.

Multi-gene phylogenetic analyses revealed two novel species and one new record of Trichobotrys (Pleosporales, Dictyosporiaceae) from China

“…The high molecular support and morphological similarities among them indicate that they are conspecific and the two isolates ( JAUCC 6359 and JAUCC 6826) are identified as a new record of T. effusa . Although four-loci data for T. effusa FS524 and T. effusa YMJ1179 were lacking and they were sequenced only by ITS, our result should be convincing because the fungal ITS marker generally produces considerably more sequence variability, and thus can provide high resolution for species delimitation ( Nilsson et al 2008 ; Szczepańska et al 2021 ). The holotype of T. effusa was discovered on dead bamboo from Sri Lanka ( Berkeley and Broome 1873 ; Petch 1924 ).…”

Figure 3 from: Zhang W-J, Xu G-P, Liu Y, Gao Y, Song H-Y, Hu H-J, Zhou J-P, Chen M-H, Fan D-M, Hu D-M, Zhai Z-J (2024) Multi-gene phylogenetic analyses revealed two novel species and one new record of Trichobotrys (Pleosporales, Dictyosporiaceae) from China. MycoKeys 106: 117-132. https://doi.org/10.3897/mycokeys.106.123279

“…The high molecular support and morphological similarities among them indicate that they are conspecific and the two isolates (JAUCC 6359 and JAUCC 6826) are identified as a new record of T. effusa. Although four-loci data for T. effusa FS524 and T. effusa YMJ1179 were lacking and they were sequenced only by ITS, our result should be convincing because the fungal ITS marker generally produces considerably more sequence variability, and thus can provide high resolution for species delimitation (Nilsson et al 2008;Szczepańska et al 2021). The holotype of T. effusa was discovered on dead bamboo from Sri Lanka (Berkeley and Broome 1873;Petch 1924).…”

Figure 1 from: Zhang W-J, Xu G-P, Liu Y, Gao Y, Song H-Y, Hu H-J, Zhou J-P, Chen M-H, Fan D-M, Hu D-M, Zhai Z-J (2024) Multi-gene phylogenetic analyses revealed two novel species and one new record of Trichobotrys (Pleosporales, Dictyosporiaceae) from China. MycoKeys 106: 117-132. https://doi.org/10.3897/mycokeys.106.123279

“…The high molecular support and morphological similarities among them indicate that they are conspecific and the two isolates (JAUCC 6359 and JAUCC 6826) are identified as a new record of T. effusa. Although four-loci data for T. effusa FS524 and T. effusa YMJ1179 were lacking and they were sequenced only by ITS, our result should be convincing because the fungal ITS marker generally produces considerably more sequence variability, and thus can provide high resolution for species delimitation (Nilsson et al 2008;Szczepańska et al 2021). The holotype of T. effusa was discovered on dead bamboo from Sri Lanka (Berkeley and Broome 1873;Petch 1924).…”