Infusion of aerial parts of Salvia chudaei Batt. & Trab. from Algeria: Chemical, toxicological and bioactivities characterization

Abstract

Salvia chudaei Batt. & Trab. from Algeria is traditionally used to relieve several dysfunctions, including inflammatory and pain-related situations.Aim of the study: This work aimed to confirm scientifically the referred properties. For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts. Materials and methods: Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn. DPPH and FRAP were used to evaluate antioxidant activity. Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested. Results: The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids. Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid. This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract. Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW). By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control. On the other hand, infusion up to 8 g/kg b.w. showed no signs of toxicity or mortality. Conclusion:This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, antiinflammatory, and analgesic activities, supporting the use of this species in folk medicine.

ANTI-INFLAMATORY AND ANTI-LIPID PEROXODATION POTENTIALS OF SALVIA CHUDAEI BATT. et TRAB. AND LAVANDULA ANTINEAE MAIRE: ENDEMIC PLANTS FROM ALGERIAN SAHARA

“…The results obtained by the methanolic extract of S. chudaei are in agreement with those obtained by Semaoui et al, 2021 who found also that the infusions prepared from this plant at doses of 250, 500 and 1000 mg/kg body weight exhibited moderate activity (45, 48 and 58%, respectively) 15 .…”

“…The remarkable anti-inflammatory activity could be attributed to the presence of phenolic acids and flavonoids present in these plants in different proportions. The chromatographic analysis carried out in the present work and also in the study of Semaoui et al, 2021 15 on S. chudaei allowed the identification of some polyphenolic components which have already been investigated by other researchers for their anti-inflammatory activity inflammatory.…”

Polyphenolic grape stalk and coffee extracts attenuate spinal cord injury-induced neuropathic pain development in ICR-CD1 female mice

“…Moreover, infusion of aerial parts of Salvia chudaei Batt. & Trab containing 4-O-caffeoylquinic acid (cryptochlorogenic acid) alleviates painful responses after carrageenan, acetic acid and formalin injection 44 . Altogether, this evidence indicates that the polyphenolic compounds present in both extracts used in this study have antinociceptive effects in different experimental models of pathological pain, although very few of them have been used in an experimental model of central neuropathic pain.…”

Ethnobotanical, Phytochemical, and Pharmacological Properties of the Subfamily Nepetoideae (Lamiaceae) in Inflammatory Diseases

“…Finally, the acetone extract and the methanol extract of S. aegyptiaca L. and S. moorcroftiana Wall. ex Benth., respectively, showed antipyretic effects in murine models of hyperthermia (Table S2) [71][72][73][74][75][76][77][78]. After the genus Salvia, the species Ocimum tenuiflorum L. (syn.…”