Ingestion of coffee polyphenols increases postprandial release of the active glucagon-like peptide-1 (GLP-1(7–36)) amide in C57BL/6J mice

Abstract

The widespread prevalence of diabetes, caused by impaired insulin secretion and insulin resistance, is now a worldwide health problem. Glucagon-like peptide 1 (GLP-1) is a major intestinal hormone that stimulates glucose-induced insulin secretion from β cells. Prolonged activation of the GLP-1 signal has been shown to attenuate diabetes in animals and human subjects. Therefore, GLP-1 secretagogues are attractive targets for the treatment of diabetes. Recent epidemiological studies have reported that an increase in daily coffee consumption lowers diabetes risk. The present study examined the hypothesis that the reduction in diabetes risk associated with coffee consumption may be mediated by the stimulation of GLP-1 release by coffee polyphenol extract (CPE). GLP-1 secretion by human enteroendocrine NCI-H716 cells was augmented in a dose-dependent manner by the addition of CPE, and was compatible with the increase in observed active GLP-1(7–36) amide levels in the portal blood after administration with CPE alone in mice. CPE increased intracellular cyclic AMP (cAMP) levels in a dose-dependent manner, but this was not mediated by G protein-coupled receptor 119 (GPR119). The oral administration of CPE increased diet (starch and glyceryl trioleate)-induced active GLP-1 secretion and decreased glucose-dependent insulinotropic polypeptide release. Although CPE administration did not affect diet-induced insulin secretion, it decreased postprandial hyperglycaemia, which indicates that higher GLP-1 levels after the ingestion of CPE may improve insulin sensitivity. We conclude that dietary coffee polyphenols augment gut-derived active GLP-1 secretion via the cAMP-dependent pathway, which may contribute to the reduced risk of type 2 diabetes associated with daily coffee consumption.

Coffee polyphenol consumption improves postprandial hyperglycemia associated with impaired vascular endothelial function in healthy male adults

“…ACCEPTED MANUSCRIPT 20 Our previous study demonstrated that coffee polyphenols increased GLP-1 secretion from enteroendocrine cells and in mice [14]. However, the present study did not observe a significant increase in blood active GLP-1 levels after coffee polyphenol ingestion.…”

“…A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 14 The initial blood parameters in the subjects were similar between the two test beverages (Table 1). The fasting levels of AST were significantly different (P < 0.05) between the beverages (Table 1).…”

“…We previously demonstrated that coffee polyphenol ingestion stimulated secretion of Glucagon-like peptide 1 (GLP-1), in both human enteroendocrine cells and mice [14]. GLP-1 is a gut-derived hormone that stimulates the glucose-induced secretion of insulin from pancreatic  cells.…”

The Impact of Coffee on Health

“…A 2.5-fold increase in glucose transport was described as an additive action with insulin [44]. Polyphenols in coffee stimulate GLP 1, which is a major intestinal hormone that activates glucose-induced insulin secretion from β-cells [45]. Prolonged activation of the GLP-1 signal has been shown to attenuate diabetes in animals and human subjects.…”

Coffee, type 2 diabetes and pancreatic islet function – A mini-review

“…Other reported T2D-related outcomes with coffee compounds include an inhibitory effect of CGAs on glucose uptake in the intestine and stimulation of glucagon-like-peptide 1 (GLP1) secretion from gastrointestinal cells, which in turn can modify gastric emptying and stimulate insulin secretion (Fujii, Osaki, Hase, & Shimotoyodome, 2015;Johnston, Clifford, & Morgan, 2003). However, contradictory results exist regarding the impact on GLP1 and thus indirectly on insulin release (Tunnicliffe, Eller, Reimer, Hittel, & Shearer, 2011).…”