Ingestion of Yogurt Containing Lactobacillus acidophilus and Bifidobacterium to Potentiate Immunoglobulin A Responses to Cholera Toxin in Mice

Abstract

Lactic acid bacteria have been reported to have benefits for the prevention and treatment of some forms of diarrhea and related conditions. To determine whether these effects might involve direct stimulation of the gastrointestinal immune response, we administered yogurt to try to enhance mucosal and systemic antibodies against an orally presented immunogen, cholera toxin. Yogurts were manufactured with starter cultures containing different species and strains of lactic acid bacteria. Mice were fed these yogurts for 3 wk, during which they were also orally immunized twice with 10 micrograms of cholera toxin. Blood was collected on d 0 and 21, and fecal pellets were collected weekly. Mice that were immunized orally with cholera toxin responded by producing specific intestinal and serum immunoglobulin (Ig)A anti-cholera toxin. Antibody responses of the IgA isotype were significantly increased in mice fed yogurts made with starters containing the conventional yogurt bacteria Lactobacillus bulgaricus and Streptococcus thermophilus supplemented with Lactobacillus acidophilus, Bifidobacterium bifidum, and Bifidobacterium infantis. Yogurt that was manufactured with starters containing only conventional yogurt bacteria produced less IgA anti-cholera toxin than did the control group fed nonfat dry milk. Although strong responses were also observed for IgG anti-cholera toxin in serum, the responses did not differ among groups. Thus, administration of yogurt supplemented with L. acidophilus and Bifidobacterium spp. enhanced mucosal and systemic IgA responses to the cholera toxin immunogen.

Effect of the Escherichia coli EMO strain on experimental infection by Salmonella enterica serovar Typhimurium in gnotobiotic mice

“…Enhancement of the amount of IgA-producing plasma cells by Lactobacillus casei or L. acidophilus has been reported (23). In addition, Tejada-Simon et al (24) showed increased IgA production in response to cholera toxin in mice treated with a yogurt containing a mixture of L. acidophilus and Bifidobacterium spp. On the other hand, studies associating the administration of non-pathogenic E. coli with the induction of antibody responses are rare and the results sometimes controversial.…”

Effect of Feeding Probiotics on Rats' Immunity and Health Conditions during Pregnancy

“…casei and Lb. acidophilus increased IgM forming cell in spleen [7,8]. B. breve strain yakult and Lb.…”

“…Many health benefits have been reported, such as immunomodulating effect which helps persons' immunity in retarding number of diseases [6][7][8], reduction of serum cholesterol [9,10], enhancing body absorption of minerals and many other health benefits. However, types of health benefits as well as their mechanisms are microorganism (type & strain) and dose dependent.…”

“…casei, Lb. acidophilus and yoghurt enhanced the number of IgA producing cells and mucosal immune response [7][8][9]. Lb.…”

Textura instrumental de queijo petit-suisse potencialmente probiótico: influência de diferentes combinações de gomas

“…A utilização conjunta de L. acidophilus e Bifidobacterium spp. tem sido estudada em leites e bebidas fermentadas [15,44], iogurtes comerciais [39] e vários tipos de queijos [29]. Dentre eles, destaca-se o queijo fresco [1,6,7,28,29,31,42].…”