Inhalant Allergy in Asthmatic Children: Skin Prick Test, Radioallergosorbent Test and Chemiluminescent Assay Compared with Allergen Levels in Their Mattress Dusts

Price, Jill A.; Reiser, J.; Longbottom, Joan L.; Warner, J. O.

doi:10.1159/000234779

Cited by 7 publications

(3 citation statements)

References 2 publications

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“…The occurrence of cat and dog allergens in places where no cats or dogs are allowed has previously been reported by us and others (1, 2, 8,12). The main allergens detected were cat, dog and mould.…”

Section: Discussionsupporting

confidence: 67%

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Dust from carpeted and smooth floors: IV. Solid material, proteins and allergens collected in the different filter stages of vacuum cleaners after ten days of use in schools.

Dybendal

Wedberg

Elsayed

1991

Allergy

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The accumulation of dust, proteins and allergens from alder, birch, timothy, cat, dog, mite, hen egg white, codfish and mould in schools was investigated by analysing the content of vacuum cleaners after 10 days of use. The main goals were to compare the dust accumulation on carpeted and smooth floors and to estimate to what degree the three vacuum cleaner filter stages (i.e. the disposable bag, the main filter and the microfilter) collected dust, proteins and allergens. Carpeted floors accumulated more dust, proteins and allergens per unit area than smooth floors. Histamine release studies of some of the dust extracts showed that the dust from carpeted floors released histamine from passively sensitized basophils at concentrations for which dust from smooth floors gave low or no histamine release. The analyses showed that most of the dust, proteins and allergens were retained in the dust bags. Less than 1 % of the vacuumed material had accumulated in the main filters, which, according to the manufacturer, detain 99.5% of particles > 2 pm. By the use of a scanning electron microscope (SEM), particle deposits were observed in the microfilters. These deposits, which represented < 0.1 % of the total mass, showed no significant allergenic activity. Thus, for the field conditions of this study, the microfilters were not needed for cleaning the exit air of allergens, although they were useful for removing fine (< 2 pm) particles. CLINICAL ASPECTSCarpeted floors accumulated more dust, proteins and allergens per unit area than smooth floors. Allergens included in dust from carpeted floors released histamine from passively sensitized basophils at concentrations for which dust from smooth floors gave low or no histamine release. Most of the dust and allergens were collected in the dust bags during vacuuming. Less than 1 % of the total vacuumed material passed through the dust bags further to the main and microfilters during vacuuming. The allergenic activities of dust from the main filter were low, even after removal of molecules with M,<3500 daltons by dialysis. By scanning electron microscopy we could clearly observe particle deposits in the third and final filter stage, the microfilter. These deposits showed little or no allergenic activity.The cleaning efficiency of commercial vacuum crease the number of airborne particles of dicleaners will usually differ from model to ameters > 0.5 pm (10). Thus, the paper bag model. Vacuum cleaning of carpets can in-used in vacuum cleaners without subsequent

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Section: Discussionsupporting

confidence: 67%

“…The main allergens detected were cat, dog and mould. The occurrence of cat and dog allergens in places where no cats or dogs are allowed has previously been reported by us and others (1, 2, 8,12). High birch inhibition RAST values could be associated with schools located in the vicinity of birch forests.…”

Section: Discussionsupporting

confidence: 56%

Dust from carpeted and smooth floors: IV. Solid material, proteins and allergens collected in the different filter stages of vacuum cleaners after ten days of use in schools.

Dybendal

Wedberg

Elsayed

1991

Allergy

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“…In this closed environment, the use of wooden furniture, wall to wall carpets, mattresses, pillows, etc., adds more suitable substrates for the development and reproduction of microflora and mites. These mites use fungi as food for their growth [1–4]. Fungi are common in both outdoor and indoor environments as spores, mycelial fragments or dissociated intracellular and extracellular components.…”

Section: Introductionmentioning

confidence: 99%

Fungal flora in house dust in Riyadh, Saudi Arabia

Parvez

1999

Mycoses

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Fungi in the house-dust of houses in the less populated and densely populated areas of Riyadh, Saudi Arabia, were screened. The screened area included drawing rooms, living rooms, kitchens, bedrooms and bathrooms. A total of 74 fungal species were isolated. The highest number of fungal colonies were found in the living rooms followed by bedrooms. The number of fungal colonies isolated were higher in densely populated areas compared with less populated areas. Aspergillus was the predominant genus represented by highest number of species followed by Penicillium.

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Diagnosis of Pediatric Allergy

Pediatric Allergy, Asthma and Immunology

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Inhalant Allergy in Asthmatic Children: Skin Prick Test, Radioallergosorbent Test and Chemiluminescent Assay Compared with Allergen Levels in Their Mattress Dusts

Cited by 7 publications

References 2 publications

Dust from carpeted and smooth floors: IV. Solid material, proteins and allergens collected in the different filter stages of vacuum cleaners after ten days of use in schools.

Dust from carpeted and smooth floors: IV. Solid material, proteins and allergens collected in the different filter stages of vacuum cleaners after ten days of use in schools.

Fungal flora in house dust in Riyadh, Saudi Arabia

Diagnosis of Pediatric Allergy

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