Inhalation Anesthetics Induce Apoptosis in Normal Peripheral Lymphocytes In Vitro

Matsuoka, Hiroshi; Kurosawa, Shin; Horinouchi, T.; Kato, Masato; Hashimoto, Yoshiaki

doi:10.1097/00000542-200112000-00028

Cited by 143 publications

(104 citation statements)

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“…Lymphocytes seemed to respond to isoflurane with early (within 4 hours) recruitment into the bone marrow, whereas surgical stress caused a marked increase in bone marrow myeloid cells 72 hours after surgery. Volatile anaesthetics reportedly affect leukocyte responses; e.g., they alter neutrophil adherence to blood vessels (Heindl et al, 1999), induce apoptosis of lymphocytes (Matsuoka et al, 2001), or affect the resolution of inflammation (Chiang et al, 2008). They also contribute to ischaemic brain injury (Lee et al, 2007;Li and Zuo, 2009), but our results are the first to show that both surgery and anaesthesia interact with experimental stroke to cause cellular and cytokine changes directly in the bone marrow.…”

Section: Discussionmentioning

confidence: 62%

Experimental Stroke-Induced Changes in the Bone Marrow Reveal Complex Regulation of Leukocyte Responses

Dénes

McColl

Leow-Dyke

et al. 2010

J Cereb Blood Flow Metab

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Stroke induces a systemic response that involves rapid activation of inflammatory cascades, followed later by immunodepression. Experimental stroke-induced responses in the bone marrow, which is the primary source of circulating monocytes and granulocytes, have not been investigated previously. We show that cerebral ischaemia induced early (4 hours) release of CXCR2-positive granulocytes from the bone marrow, which was associated with rapid systemic upregulation of CXCL1 (a ligand for CXCR2) and granulocyte-colony-stimulating factor, a key cytokine involved in the mobilisation of bone marrow leukocytes. This process involves rapid activation of nuclear factor-jB and p38 mitogen-activated protein kinase in bone marrow myeloid cells. T-cell numbers in the bone marrow increased after stroke, and bone marrow cells did not show suppressed cytokine response to bacterial endotoxin stimulation in vitro. Stroke-induced laterality observed in the brain stem and in the bone marrow indicates direct involvement of the autonomic nervous system in stroke-induced cell mobilisation. We also show that systemic inflammatory changes and leukocyte responses in the bone marrow are profoundly affected by both anaesthetic and surgical stress. We conclude that stroke influences leukocyte responses in the bone marrow through multiple mechanisms and suggest that preclinical studies should take into consideration the effect of surgical manipulation in experimental models of stroke.

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Section: Discussionmentioning

confidence: 62%

Experimental Stroke-Induced Changes in the Bone Marrow Reveal Complex Regulation of Leukocyte Responses

Dénes

McColl

Leow-Dyke

et al. 2010

J Cereb Blood Flow Metab

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show abstract

“…In addition, it has been reported that NK activity decreases and that the reactivity between NK cells and interferon-γ is suppressed after anesthesia in humans [5]. Third, there may be a direct action of isoflurane on lymphocytes, because a decrease in NK cytotoxic activity has been reported in an ex vivo experiment in which human lymphocytes were exposed to isoflurane [8]. However, it has also been reported that the site of the cytotoxic action of isoflurane on lymphocytes is not the membrane.…”

Section: Discussionmentioning

confidence: 99%

“…For example, isoflurane decreases cytokine production in lymphocytes and NK cell activity [7] and can also induce apoptosis of lymphocytes [8]. To our knowledge, effects of anesthetics on anti-tumor immunity have not been reported for veterinary medicine.…”

mentioning

confidence: 98%

Influence of General Anesthesia with Isoflurane Following Propofol-Induction on Natural Killer Cell Cytotoxic Activities of Peripheral Blood Lymphocytes in Dogs

et al. 2013

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ABSTRACT. To investigate influence of general anesthesia on immunological anti-tumor activity, the natural killer (NK) cytotoxic activity of peripheral lymphocytes (PBLs) was measured in 7 dogs anesthetized for 3 hr with isoflurane following propofol-induction (anesthesia group) and 6 dogs without anesthesia (control group). Blood samples were collected before (baseline) and 24, 120 and 192 hr after the anesthesia. The PBLs were isolated via centrifugation with Ficoll-Hypaque solution (density, 1.073), and adherent cells were removed. The NK cytotoxic activity of the isolated PBLs against canine thyroid cancer cells was detected by the colorimetric rose Bengal assay. Significant decrease in the NK cytotoxic activity was observed at 24 hr after the anesthesia, compared with the baseline values and the control group. The NK cytotoxic activities were recovered to the baseline values until 120 hr after the anesthesia. The general anesthesia with isoflurane following propofol-induction decreased the NK cytotoxic activities of PBLs in dogs. This finding has a clinical relevance to the risk of tumor recurrence or metastasis induced by the suppression of immunological anti-tumor activity after general anesthesia in dogs. The results further emphasized the importance of the need to evaluate immune suppression following general anesthesia in animals.

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“…It was shown that the induction of apoptosis is accompanied by increased levels of activated caspase-3 (CASP3) in the analyzed lymphatic cells (43). At the same time, it was shown that isoflurane limphotoxicity was greater than sevoflurane limphotoxicity.…”

Section: The Impact Of Volatile Anaesthetics On the Function Of Lymphmentioning

confidence: 95%

“…In vitro, isoflurane and sevoflurane directly induce the phenomenon of apoptosis in human peripheral blood lymphocytes depending on a dose and the duration of exposure (43).…”

Section: The Impact Of Volatile Anaesthetics On the Function Of Lymphmentioning

confidence: 99%

The Anti-Inflammatory and Immunosuppressive Properties of Inhaled Anaesthetics - Data from Experimental Studies and Clinical Implications

2017

ARC Journal of Anesthesiology

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Inhalation Anesthetics Induce Apoptosis in Normal Peripheral Lymphocytes In Vitro

Abstract: Both sevoflurane and isoflurane induced apoptosis in peripheral lymphocytes in dose-dependent and time-dependent manners in vitro.

Cited by 143 publications

References 0 publications

Experimental Stroke-Induced Changes in the Bone Marrow Reveal Complex Regulation of Leukocyte Responses

Experimental Stroke-Induced Changes in the Bone Marrow Reveal Complex Regulation of Leukocyte Responses

Influence of General Anesthesia with Isoflurane Following Propofol-Induction on Natural Killer Cell Cytotoxic Activities of Peripheral Blood Lymphocytes in Dogs

The Anti-Inflammatory and Immunosuppressive Properties of Inhaled Anaesthetics - Data from Experimental Studies and Clinical Implications

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