Inhalation exposure to crude oil vapor induces behavioural deficits by increasing oxidative stress and histopathological changes in rat hippocampus: Quercetin therapeutic approach

Sadeghi, Abbas; Ghahari, Laya; Yousefpour, Mitra; Khazaeel, Kaveh; Zareian, Parvin

doi:10.1016/j.jchemneu.2023.102290

Cited by 8 publications

(2 citation statements)

References 42 publications

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“…In this section, we report how we determined our sample size, all data exclusions (if any), all manipulations, and all measures in the study. Samples (rats) were randomly selected and sample size in each group was selected based on previous research (Sadeghi et al, 2023; Zhang et al, 2023). Data were analyzed using SPSS software (V.26; Kholghi et al, 2023) and are available upon a reasonable request.…”

Section: Methodsmentioning

confidence: 99%

Psilocybe cubensis extract potently prevents fear memory recall and freezing behavior in short- but not long-term in a rat model of posttraumatic stress disorder.

Ghofrani-Jahromi,

Nouri-Darehno,

Rahimi-Danesh

et al. 2024

Behavioral Neuroscience

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Psilocybe cubensis is a species of psilocybin mushroom (magic mushroom) of moderate potency whose principal active compounds are psilocybin and psilocin. Recent studies have shown the significant procognitive and mood-enhancer effects of Psilocybe cubensis. However, evidence is so limited, especially in preclinical studies. We aimed to investigate the effect of Psilocybe cubensis extract on posttraumatic stress disorder (PTSD)-like behavior, pain perception, locomotor activity, and anxiety in a rat model of PTSD. Male rats were exposed to three consecutive shocks (0.8 mA, 3 s interval) paired with three sounds broadcasted 3 s before delivering shocks (75 dB, 3 s). After 1, 3, or 21 days, freezing rate was measured in the fear-conditioning apparatus. Open filed test and hot plate were used to assess locomotor activity and anxiety, and pain subthreshold, respectively. Psilocybe cubensis was injected intraperitoneal at the dose of 25 mg/kg (single administration) before (pretrain) or after (posttrain) shocks, or before the test (pretest). Results showed psilocybin potently alleviated PTSD symptom is short-but not long-term after the induction of PTSD. Psilocybe cubensis decreased locomotor activity only in a short period after administration. Psilocybe cubensis also increased pain subthreshold and decreased anxiety. In conclusion, Psilocybe cubensis effects on PTSD-like behavior and locomotor activity seem to be remained in short-term, while Psilocybe cubensis effects on pain subthreshold and anxiety remained long-term. This is the first study evaluating the effect of Psilocybe cubensis on PTSD-like behavior in rats in three different time protocols (1, 3, and 21 days after fear conditioning).

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Section: Methodsmentioning

confidence: 99%

Psilocybe cubensis extract potently prevents fear memory recall and freezing behavior in short- but not long-term in a rat model of posttraumatic stress disorder.

Ghofrani-Jahromi,

Nouri-Darehno,

Rahimi-Danesh

et al. 2024

Behavioral Neuroscience

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“…However, it has been shown that gasoline derived from crude oil has an inherent potential to generate reactive oxygen species (ROS) (Uboh et al, 2012). Recently, it has been shown that the developmental toxicological mechanism of crude oil pollutants includes the production of ROS and increased oxidative stress, which causes apoptosis and cellular oxidative damage (Sadeghi et al, 2023). The developing fetus is highly sensitive to oxidative stress due to rapid cell division and immature antioxidant defense systems.…”

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confidence: 99%

Protective effect of quercetin on fetal development and congenital skeletal anomalies against exposure of pregnant Wistar rats to crude oil vapor

Hussein,

Khazaeel,

Ranjbar

et al. 2023

Birth Defects Research

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BackgroundEpidemiological evidence indicates a relationship between maternal exposure to crude oil vapors (COV) during pregnancy and adverse pregnancy outcomes. Quercetin (QUE) is a plant flavonoid with purported antioxidant and anti‐inflammatory effects, which has been shown to prevent birth defects. This study was aimed to investigate the protective role of QUE on fetal development and congenital skeletal anomalies caused by exposure of pregnant rats to COV.MethodsTwenty‐four pregnant Wistar rats were randomly categorized into four groups of control, COV, COV + QUE, and QUE (50 mg/kg). The inhalation method was used to expose pregnant rats to COV from day 0 to 20 of pregnancy, and QUE was administered orally during this period. On day 20 of gestation, the animals were anesthetized and a laparotomy was performed, and then the weight and crown rump length (CRL) of the fetuses were determined. Skeletal stereomicroscopic evaluations of fetuses were performed using Alcian blue/Alizarin red staining method, and the expression of osteogenesis‐related genes (Runx2 and BMP‐4) was evaluated using qPCR.ResultsThis study showed that prenatal exposure to COV significantly reduced fetal weight and CRL, and expression of Runx2 and BMP‐4 genes. Moreover, COV significantly increased the incidence of congenital skeletal anomalies such as cleft palate, spina bifida and non‐ossification of the fetal bones. However, administration of QUE with exposure to COV improved fetal bone development and reduced congenital skeletal anomalies.ConclusionQUE can ameliorate the teratogenic effects of prenatal exposure to COV by increasing the expression of osteogenesis‐related genes.

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Prenatal exposure to crude oil vapor reduces differentiation potential of rat fetal mesenchymal stem cells by regulating ERK1/2 and PI3K signaling pathways: Protective effect of quercetin

Sadeghi

Khazaeel

Tabandeh

et al. 2023

Reproductive Toxicology

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Inhalation exposure to crude oil vapor induces behavioural deficits by increasing oxidative stress and histopathological changes in rat hippocampus: Quercetin therapeutic approach

Cited by 8 publications

References 42 publications

Psilocybe cubensis extract potently prevents fear memory recall and freezing behavior in short- but not long-term in a rat model of posttraumatic stress disorder.

Psilocybe cubensis extract potently prevents fear memory recall and freezing behavior in short- but not long-term in a rat model of posttraumatic stress disorder.

Protective effect of quercetin on fetal development and congenital skeletal anomalies against exposure of pregnant Wistar rats to crude oil vapor

Prenatal exposure to crude oil vapor reduces differentiation potential of rat fetal mesenchymal stem cells by regulating ERK1/2 and PI3K signaling pathways: Protective effect of quercetin

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