Inhalation of methane preserves the epithelial barrier during ischemia and reperfusion in the rat small intestine

Mészáros, András; Buki, Tamas; Fazekas, Borbála; Tuboly, Eszter; Horváth, Krisztina; Poles, Marietta Zita; Szücs, S; Varga, Gabriella; Kaszaki, József; Boros, Mihály

doi:10.1016/j.surg.2016.12.040

Cited by 23 publications

(13 citation statements)

References 47 publications

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“…In our study, myeloperoxidase (MPO) whose activity is assessed during evaluation of intestinal ischemic injury, was shown to be an enzyme released due to neutrophil activation and is present during the production of free oxygen radicals [15,16]. Various studies have demonstrated that increase in MPO activity as a sign of neutrophil infiltration during mesenteric I/R injury causes endothelia dysfunction and inflammation [17,18]. In our study, MPO values increased significantly with I/R, consistent with literature results.…”

Section: Discussionsupporting

confidence: 90%

The Protective Effect of Spironolactone and Role of the Na /K -ATPase Pump on Intestinal Ischemia/Reperfusion Injury

Akyüz¹,

Uzun²,

Sunamak³

et al. 2018

jrp

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The aim of this study was to evaluate the possible protective effect of spironolactone (SPL) and role of the Na-K ATPase pump on intestinal ischemia/reperfusion injury. In our study, the period of ischemia was established by clamping the mesenteric artery for 45 minunder anesthesia in Wistar albino rats and the animals left for reperfusion at the end of this period were decapitated after one hour. Spironolactone (20 mg kg-1) was administered orally for three days before ischemia, 30 minbefore ischemia. The control group rats were subjected to the Sham operation and administered saline solution. TNF-α and IL-1β levels were measured in the serum samples. Ileal Na+/K+-ATPase, myeloperoxidase (MPO) analysis were performed. Structural injury was assessed histopathologically. Ischemia/reperfusion increased serum TNF-α and IL-1β levels together with MPO activity, whereas these values were maintained at the control group levels through SPL activation. However, ischemia/reperfusion decreased Na+/K+-ATPase activity in ileal tissues; however, these parameters were found to be significantly increased with SPL activation. The protective effect of SPL against ischemia/reperfusion injury by different mechanisms, mainly the activity of the Na+/K+-ATPase pump, suggests that this nontoxic agent may constitute a new clinical therapeutic principle.

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Section: Discussionsupporting

confidence: 90%

The Protective Effect of Spironolactone and Role of the Na /K -ATPase Pump on Intestinal Ischemia/Reperfusion Injury

Akyüz¹,

Uzun²,

Sunamak³

et al. 2018

jrp

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show abstract

“…In this study, such analyses indicated that the circulating microbiome of NACs were enriched with respect to methanogenesis, denitrification, and several other functions. A growing body of literature indicates an important role of gases as “signaling” molecules, and both methane and hydrogen sulfide have been implicated as regulators of intestinal epithelial integrity and gut‐derived inflammatory signals . A likely possibility is that circulating bacteria, by producing even minute quantities of such gases, and/or pathogen‐associated molecular patterns may modulate signaling within the innate and adaptive immune system, modify oxidative stress, and affect the microcirculation and even cellular function in individual organs.…”

Section: Discussionmentioning

confidence: 99%

“…A growing body of literature indicates an important role of gases as "signaling" molecules, and both methane and hydrogen sulfide have been implicated as regulators of intestinal epithelial integrity and gut-derived inflammatory signals. (35,36) A likely possibility is that circulating bacteria, by producing even minute quantities of such gases, and/or pathogen-associated molecular patterns may modulate signaling within the innate and adaptive immune system, modify oxidative stress, and affect the microcirculation and even cellular function in individual organs. This possibility is further supported by the well-established roles of nitric oxide and carbon monoxide as modulators of endothelial dysfunction, microcirculation, organ function, and inflammation, particularly in the liver.…”

Section: Discussionmentioning

confidence: 99%

The circulating microbiome signature and inferred functional metagenomics in alcoholic hepatitis

et al. 2018

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“…The in vitro results substantiated the in vivo findings, and established that CH 4 exposure specifically decreases the ROS production of activated PMN leukocytes (Boros et al, 2012). In another study normoxic ventilation with 2.5% CH 4 maintained the superficial mucosal structure, the reperfusion-induced epithelial hyperpermeability was significantly alleviated and the microcirculatory flow reduction was prevented (Mészáros et al, 2017a).…”

Section: Anti-inflammatory and Anti-apoptotic Effects During Ischemiamentioning

confidence: 99%

Methane Production and Bioactivity-A Link to Oxido-Reductive Stress

Boros

Keppler

2019

Front. Physiol.

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Biological methane formation is associated with anoxic environments and the activity of anaerobic prokaryotes (Archaea). However, recent studies have confirmed methane release from eukaryotes, including plants, fungi, and animals, even in the absence of microbes and in the presence of oxygen. Furthermore, it was found that aerobic methane emission in plants is stimulated by a variety of environmental stress factors, leading to reactive oxygen species (ROS) generation. Further research presented evidence that molecules with sulfur and nitrogen bonded methyl groups such as methionine or choline are carbon precursors of aerobic methane formation. Once generated, methane is widely considered to be physiologically inert in eukaryotes, but several studies have found association between mammalian methanogenesis and gastrointestinal (GI) motility changes. In addition, a number of recent reports demonstrated anti-inflammatory potential for exogenous methane-based approaches in model anoxia-reoxygenation experiments. It has also been convincingly demonstrated that methane can influence the downstream effectors of transiently increased ROS levels, including mitochondria-related pro-apoptotic pathways during ischemia-reperfusion (IR) conditions. Besides, exogenous methane can modify the outcome of gasotransmitter-mediated events in plants, and it appears that similar mechanism might be active in mammals as well. This review summarizes the relevant literature on methane-producing processes in eukaryotes, and the available results that underscore its bioactivity. The current evidences suggest that methane liberation and biological effectiveness are both linked to cellular redox regulation. The data collectively imply that exogenous methane influences the regulatory mechanisms and signaling pathways involved in oxidative and nitrosative stress responses, which suggests a modulator role for methane in hypoxia-linked pathologies.

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Inhalation of methane preserves the epithelial barrier during ischemia and reperfusion in the rat small intestine

Cited by 23 publications

References 47 publications

The Protective Effect of Spironolactone and Role of the Na /K -ATPase Pump on Intestinal Ischemia/Reperfusion Injury

The Protective Effect of Spironolactone and Role of the Na /K -ATPase Pump on Intestinal Ischemia/Reperfusion Injury

The circulating microbiome signature and inferred functional metagenomics in alcoholic hepatitis

Methane Production and Bioactivity-A Link to Oxido-Reductive Stress

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