Inhalation toxicity of thermal transformation products formed from e-cigarette vehicle liquid using an in vitro lung model exposed at the Air–Liquid Interface

Ruth, T.; Daniel, J.; König, A.; Trittler, R.; Garcia-Käufer, M.

doi:10.1016/j.fct.2023.114157

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…Biological limitations include i) the use of the A549 human lung cell line. Despite its widespread use as in vitro lung model for ALI experiments [ 31 , 42 ], there are drawbacks compared to primary cells. For example, A549 cells lack the ability to form tight junctions [ 35 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…Forty-eight hours before exposure, A549 cells were seeded on the apical side of 6-well-sized inserts (4.524 cm 2 area, pore size 0.4 μm, transparent membrane, Greiner Bio-One, Frickenhausen, Germany) at a density of 60.000 cells/cm 2 in 1 mL growth medium ( Fig 3 ), according to Ruth et al [ 42 ]. Before seeding, inserts without cells were placed into microplates (CytoOne, Starlab GmbH) filled with 1.5 mL growth medium in the basal compartment and were incubated for 30 min in the incubator to condition the membranes of the inserts.…”

Section: Methodsmentioning

confidence: 99%

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Air-liquid interface exposure of A549 human lung cells to characterize the hazard potential of a gaseous bio-hybrid fuel blend

Daniel,

Schönberger Alvarez,

te Heesen

et al. 2024

PLoS ONE

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Gaseous and semi-volatile organic compounds emitted by the transport sector contribute to air pollution and have adverse effects on human health. To reduce harmful effects to the environment as well as to humans, renewable and sustainable bio-hybrid fuels are explored and investigated in the cluster of excellence “The Fuel Science Center” at RWTH Aachen University. However, data on the effects of bio-hybrid fuels on human health is scarce, leaving a data gap regarding their hazard potential. To help close this data gap, this study investigates potential toxic effects of a Ketone-Ester-Alcohol-Alkane (KEAA) fuel blend on A549 human lung cells. Experiments were performed using a commercially available air-liquid interface exposure system which was optimized beforehand. Then, cells were exposed at the air-liquid interface to 50–2000 ppm C3.7 of gaseous KEAA for 1 h. After a 24 h recovery period in the incubator, cells treated with 500 ppm C3.7 KEAA showed significant lower metabolic activity and cells treated with 50, 250, 500 and 1000 ppm C3.7 KEAA showed significant higher cytotoxicity compared to controls. Our data support the international occupational exposure limits of the single KEAA constituents. This finding applies only to the exposure scenario tested in this study and is difficult to extrapolate to the complex in vivo situation.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Air-liquid interface exposure of A549 human lung cells to characterize the hazard potential of a gaseous bio-hybrid fuel blend

Daniel,

Schönberger Alvarez,

te Heesen

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Forty-eight hours before exposure, A549 cells were seeded on the apical side of 6-well-sized inserts (4.524 cm² area, pore size 0.4 µm, transparent membrane, Greiner Bio-One, Frickenhausen, Germany) at a density of 60.000 cells/cm² in 1 mL growth medium (Fig 3), according to Ruth et al [27]. Before seeding, inserts without cells were placed into microplates (CytoOne, Starlab GmbH) filled with 1.5 mL growth medium in the basal compartment and were incubated for 30 min in the incubator to condition the membranes of the inserts.…”

Section: Methodsmentioning

confidence: 99%

Air-liquid interface exposure of A549 human lung cells to characterize the hazard potential of a gaseous bio-hybrid fuel blend

Daniel,

Schönberger Alvarez,

te Heesen

et al. 2024

Preprint

View full text Add to dashboard Cite

Gaseous and semi-volatile organic compounds emitted by the transport sector contribute to air pollution and have adverse effects on human health. To reduce harmful effects to the environment as well as to humans, renewable and sustainable bio-hybrid fuels are explored and investigated in the cluster of excellence “The Fuel Science Center” at RWTH Aachen University. However, data on the effects of bio-hybrid fuels on human health is scarce, leaving a data gap regarding their hazard potential. To help close this data gap, this study investigates potential toxic effects of a Ketone-Ester-Alcohol-Alkane (KEAA) fuel blend on A549 human lung cells. Experiments were performed using a commercially available air-liquid interface exposure system which was optimized beforehand. Then, cells were exposed at the air-liquid interface to 50-2000 ppm C3.7of gaseous KEAA for 1 h. After a 24 h recovery period in the incubator metabolic activity and cytotoxicity of cells were assessed. Our data support the international occupational exposure limits of the single KEAA constituents and moreover indicate no adverse effect to A549 cells when exposed to a fuel mixture. This finding applies only to the exposure scenario tested in this study and is difficult to extrapolate to the complexin vivosituation.

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An examination of the methods and variables used in experimental design that impact the toxicological outcomes of e-cigarettes

Beard,

Sayes

2024

Food and Chemical Toxicology

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Inhalation toxicity of thermal transformation products formed from e-cigarette vehicle liquid using an in vitro lung model exposed at the Air–Liquid Interface

Cited by 4 publications

References 45 publications

Air-liquid interface exposure of A549 human lung cells to characterize the hazard potential of a gaseous bio-hybrid fuel blend

Air-liquid interface exposure of A549 human lung cells to characterize the hazard potential of a gaseous bio-hybrid fuel blend

Air-liquid interface exposure of A549 human lung cells to characterize the hazard potential of a gaseous bio-hybrid fuel blend

An examination of the methods and variables used in experimental design that impact the toxicological outcomes of e-cigarettes

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