Inhaled carbon monoxide reduces leukocytosis in a murine model of sickle cell disease

Abstract

GM. Inhaled carbon monoxide reduces leukocytosis in a murine model of sickle cell disease.

A novel hemoglobin-binding peptide reduces cell-free hemoglobin in murine hemolytic anemia

“…For example, the cytoprotective enzyme heme oxygenase-1 (HO-1) is induced by its substrate (heme) (34,54). Others have demonstrated that potentiation of HO-1 expression or administration of HO-1 enzymatic products is protective in SS mice (4,6,7). Further studies are required to elucidate the mechanisms by which hE-Hb-B10 reduces liver injury as well as to identify any additional potential therapeutic effects of this peptide for treatment of hemolytic anemia.…”

The effects of chronic carbon monoxide treatment on diet-induced obesity and Rev-erb-alpha target gene expression in adipose tissue

“…The present study clearly demonstrates that CO inhalation does not alter the expression of Reverb-α targets in adipose tissue of obese mice. It is unlikely that the gas delivery method explains the ineffectiveness of CO treatment on the expression of Rev-erb-α targets because CO inhalation has been shown to stimulate PGC-1α dependent gene expression in skeletal muscle and anti-inflammatory signaling in liver [17,28]. Furthermore, we observed an increase in HO-1 mRNA expression in adipose tissue in HFD-fed mice that were treated with CO.…”

“…Because CO treatment, whether administered by gas inhalation or CO releasing molecules, increases HO-1 expression and activity [8,17,20], we used HO-1 mRNA expression in EWAT as a positive control to demonstrate that our CO gas treatment regimen was effective in adipose tissue. As expected, treating mice with CO gas (250 ppm, 1 hr/day) for six weeks resulted in a significant increase in HO-1 mRNA expression in EWAT from mice fed a HFD (Figure 3).…”

“…Following 15 weeks of the dietary intervention, HFD-fed mice were body weight-matched-assigned to a CO treatment group (HFD-CO) or control group. Mice from the HFD-CO group were place in individual induction chambers where they received CO gas (250 ppm) for 60 min per day for 40 consecutive days [17,18]. This CO concentration is slightly higher than what has been show to increase carboxyhemoglobin levels [9].…”

Emerging drugs for sickle cell anemia

“…Carbon monoxide inhibits inflammation and hypoxia-induced vasoocclusion in transgenic mice [67]. MP4-CO is a human Hb conjugated to carbon monoxide via an MP4 molecule.…”