Gas cylinders are widely used in research laboratories, pilot plants, and manufacturing industries because of their affordability, safety, and ease of access as reliable high-pressure gas sources. This review offers a concise summary of gas cylinders, focusing on three significant topics: the categorization of gas cylinders, the color coding of gas cylinders, and the components and identification of gas cylinders. Additionally, it offers guidance on the safe storage, transportation, and use of gas cylinders as well as inspection protocols for gas cylinders in university laboratories in China. Finally, it underlines the hazards that incorrect handling of gas cylinders can cause. By spreading awareness of gas cylinders, we intend to give a more comprehensive audience access to basic knowledge about gas cylinders, lowering the incidence of accidents caused by incorrect handling.