Inheritance and variation of erucic acid content in a transgenic rapeseed (Brassica napus L.) doubled haploid population

Nath, Ujjal Kumar; Goswami, Gunajit; Clemens, Rosemarie; Becker, Heiko C.; Möllers, Christian

doi:10.1007/s11032-008-9220-4

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…Similar findings were reported by Rahman et al [12]. In 2002 and 2004, one SNP was found in FAE1 between a high and a low erucic acid content B. napus cultivar [13,14], and this was also reported by Nath et al [15]. In addition, a four-base nucleotide deletion within the FAE1 coding region in an LEA compared to an HEA cultivar was reported.…”

Section: Introductionsupporting

confidence: 84%

Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents

et al. 2010

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BackgroundFAE1 (fatty acid elongase1) is the key gene in the control of erucic acid synthesis in seeds of Brassica species. Due to oil with low erucic acid (LEA) content is essential for human health and not enough LEA resource could be available, thus new LEA genetic resources are being sought for Brassica breeding. EcoTILLING, a powerful genotyping method, can readily be used to identify polymorphisms in Brassica.ResultsSeven B. rapa, nine B. oleracea and 101 B. napus accessions were collected for identification of FAE1 polymorphisms. Three polymorphisms were detected in the two FAE1 paralogues of B. napus using EcoTILLING and were found to be strongly associated with differences in the erucic acid contents of seeds. In genomic FAE1 sequences obtained from seven B. rapa accessions, one SNP in the coding region was deduced to cause loss of gene function. Molecular evolution analysis of FAE1 homologues showed that the relationship between the Brassica A and C genomes is closer than that between the A/C genomes and Arabidopsis genome. Alignment of the coding sequences of these FAE1 homologues indicated that 18 SNPs differed between the A and C genomes and could be used as genome-specific markers in Brassica.ConclusionThis study showed the applicability of EcoTILLING for detecting gene polymorphisms in Brassica. The association between B. napus FAE1 polymorphisms and the erucic acid contents of seeds may provide useful guidance for LEA breeding. The discovery of the LEA resource in B. rapa can be exploited in Brasscia cultivation.

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Section: Introductionsupporting

confidence: 84%

Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents

et al. 2010

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“…Examples in oilseed rape include selection of lines for phytosterol content (Amar et al. , 2008), erucic acid content (Nath et al. , 2009), plant height (Cheng et al.…”

Section: Exploitationmentioning

confidence: 99%

Haploids in flowering plants: origins and exploitation

Dunwell

2010

Plant Biotechnology Journal

364

277

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SummaryThe first haploid angiosperm, a dwarf form of cotton with half the normal chromosome complement, was discovered in 1920, and in the ninety years since then such plants have been identified in many other species. They can occur either spontaneously or can be induced by modified pollination methods in vivo, or by in vitro culture of immature male or female gametophytes. Haploids represent an immediate, one-stage route to homozygous diploids and thence to F 1 hybrid production. The commercial exploitation of heterosis in such F 1 hybrids leads to the development of hybrid seed companies and subsequently to the GM revolution in agriculture. This review describes the range of techniques available for the isolation or induction of haploids and discusses their value in a range of areas, from fundamental research on mutant isolation and transformation, through to applied aspects of quantitative genetics and plant breeding. It will also focus on how molecular methods have been used recently to explore some of the underlying aspects of this fascinating developmental phenomenon.

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“…Despite the opportunities for exploiting high erucic rapeseed oil as a renewable feedstock, growing demand from the oleochemical industry and its widespread cultivation, there has been relatively little progress increasing the proportion of erucic acid in oil produced commercially (Iakovlieva et al, 2017;Johnson and Fritz, 1989;Knutsen et al, 2016;Meakin, 2007;Nieschlag and Wolff, 1971;R€ obbelen, 1991;Zanetti et al, 2012). Transgenic approaches have enabled exploration of one limitation in the accumulation of erucic acid in storage lipid, that imposed by the Brassica LPAAT enzyme, which cannot incorporate VLCFAs at the sn-2 position of TAG (Bernerth and Frentzen, 1990;Brockerhoff, 1971;Cao et al, 1990;Frentzen, 1993;Katavic et al, 2001;Nath, 2008;Nath et al, 2007;Nath et al, 2009;Sasongko and M€ ollers, 2005). The analysis we undertook of FA composition at TAG sn-2 positions (Table S2) confirmed expectations that even in the presence of additional substrate, VLCFAs are not incorporated at this position.…”

Section: Discussionmentioning

confidence: 99%

The impact of reducing fatty acid desaturation on the composition and thermal stability of rapeseed oil

Kaur

Wang

Stawniak

et al. 2019

Plant Biotechnology Journal

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Summary Oilseed rape (Brassica napus) is the third largest source of vegetable oil globally. In addition to food uses, there are industrial applications that exploit the ability of the species to accumulate the very‐long‐chain fatty acid (VLCFA) erucic acid in its seed oil, controlled by orthologues of FATTY ACID ELONGASE 1 (Bna.FAE1.A8 and Bna.FAE1.C3). The proportion of polyunsaturated fatty acids (PUFAs) in rapeseed oil is predicted to affect its thermal stability and is controlled by orthologues of FATTY ACID DESATURASE 2, particularly Bna.FAD2.C5. Our aim was to develop rapeseed lines combining high erucic and low PUFA characters and to assess the impact on thermal stability of the oil they produce. The new type of rapeseed oil (high erucic low polyunsaturate; HELP) contained a substantially greater proportion of erucic acid (54%) compared with high erucic rapeseed oil (46%). Although the total VLCFA content was greater in oil from HELP lines (64%) than from high erucic rapeseed (57%), analysis of triacylglycerol composition showed negligible incorporation of VLCFAs into the sn‐2 position. Rancimat analysis showed that the thermal stability of rapeseed oil was improved greatly as a consequence of reduction of PUFA content, from 3.8 and 4.2 h in conventional low erucic and high erucic rapeseed oils, respectively, to 11.3 and 16.4 h in high oleic low PUFA (HOLP) and HELP oils, respectively. Our results demonstrate that engineering of the lipid biosynthetic pathway of rapeseed, using traditional approaches, enables the production of renewable industrial oils with novel composition and properties.

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Inheritance and variation of erucic acid content in a transgenic rapeseed (Brassica napus L.) doubled haploid population

Cited by 7 publications

References 32 publications

Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents

Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents

Haploids in flowering plants: origins and exploitation

The impact of reducing fatty acid desaturation on the composition and thermal stability of rapeseed oil

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