Inheritance of Arabidopsis DNA in offspring from Brassica napus and A. thaliana somatic hybrids

Bohman, Svante; Forsberg, Johanna; Glimelius, Kristina; Dixelius, Christina

doi:10.1007/s001220051045

Cited by 13 publications

(8 citation statements)

References 27 publications

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“…Although the primary hybrids contained most chromosomes from both species (Forsberg et al 1998), recurrent backcrossing to B. napus appears to sort out the A. thaliana DNA rapidly. This finding is in accordance with Bohman et al (1999) who also showed that translocations in this material are rare events. The phenotypic stability within lines and between generations in the present study supports the assumption of minimal presence of A. thaliana nuclear DNA.…”

Section: Discussionsupporting

confidence: 95%

“…The novel cytoplasmic composition established in the hybrid plant remains essentially unchanged during subsequent sexual crossings and is maternally inherited. Recurrent back-crossing to either one of the parents rapidly results in elimination of the nuclear DNA of the other parent (Bohman et al 1999). Thus, alloplasmic hybrids can be obtained with the nuclear genome from one species, with a rearranged mitochondrial genome including DNA from both species and with either of the parental chloroplast genomes.…”

Section: Introductionmentioning

confidence: 98%

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Brassica napus lines with rearranged Arabidopsis mitochondria display CMS and a range of developmental aberrations

et al. 2002

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Numerous Brassica napus (+) Arabidopsis thaliana somatic hybrids were screened for male sterility and aberrant flower phenotypes. Nine hybrids were selected and backcrossed recurrently to B. napus. The resulting lines displayed stable maternal inheritance of flower phenotypes. Nuclear and organellar genomes were characterized molecularly using RFLP analysis. No DNA from A. thaliana was found in the nuclear genome after six back-crosses, whilst the mitochondrial genomes contained rearranged DNA from both A. thaliana and B. napus. Each line tested had a unique RFLP pattern of the mitochondrial DNA (mtDNA) that remained unchanged between the BC(3) and BC(6) generation. The plastid genomes consisted of B. napus DNA. Five lines of the BC(5) generation were subjected to more comprehensive investigations of growth, morphology and fertility. On the basis of these investigations, the five CMS lines could be assigned to two groups, one represented by three lines displaying reduced vegetative development, complete male sterility, and homeotic conversions of stamens into feminized structures. The second group, represented by the other two lines, were not completely male-sterile but still displayed severely affected flower morphologies. These two lines did not display any reduction in vegetative development. For both groups only stamens and petals suffered from the morphological and functional aberrations, while the sepals and pistils displayed normal morphology. All plants were fully female-fertile. Different rearrangements of the mitochondrial genome disturbed nuclear-mitochondrial interactions and led to various types of aberrant growth and flower development. The existence of numerous CMS lines with different mitochondrial patterns involving a species with a sequenced genome offers new opportunities to investigate the genetic regulation of CMS and its associated developmental perturbations.

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Section: Discussionsupporting

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Brassica napus lines with rearranged Arabidopsis mitochondria display CMS and a range of developmental aberrations

et al. 2002

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“…Somatic hybrids with all five A. thaliana chromosome pairs present together with the B. napus genome, were backcrossed once and selfed four-times during the study (Table 1). Asymmetric hybrids between B. napus and A. thaliana, Landsberg erecta (Ler-0), selected for integrated fragments of A. thaliana chromosome 3 and with approximately 38 chromosomes (Forsberg et al 1998a, b;Bohman et al 1999), were analysed in two backcrossed generations. The parents of the somatic hybrid plants, B. napus cv Hanna, A. thaliana Col-0 and Ler-0, were used as control groups in all experiments.…”

Section: Plant Materialsmentioning

confidence: 99%

Arabidopsis thaliana-derived resistance against Leptosphaeria maculans in a Brassica napus genomic background

2002

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Stem canker (blackleg) caused by Leptosphaeria maculans is a widespread disease of Brassica napus. In contrast, most Arabidopsis thaliana accessions are highly resistant. Hence, novel material derived from symmetric and asymmetric somatic hybrids between B. napus and A. thaliana was utilised in a screen for L. maculans resistance. Initially, both cotyledon and adult-leaf resistance traits were transferred from A. thaliana to B. napus. In later generations the two traits segregated and cotyledon resistance was lost. The adult-leaf resistance was investigated with respect to genome localisation and protein expression. Analyses of remaining A. thaliana DNA in resistant plants showed co-segregation between adult-leaf resistance and chromosome-3 molecular markers. Resistant offspring from asymmetric hybrid plants that contained fragments of chromosome 3 were studied in more detail. Two regions at positions 9.8-10.4 Mbp and 18-19.5 Mbp, where several defence-related genes are located, were identified. A proteomic approach was taken to further investigate genes involved in the defence interaction. Forty eight hours after inoculation with L. maculans, only a few proteins, such as glycolate oxidase, were identified as differentially expressed in the resistant line compared to B. napus, despite the presence of additional A. thaliana chromosomes. The plant materials described in the present study constitute a new genetic source of L. maculans resistance and are currently being incorporated into B. napus breeding programmes.

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“…Indeed Bohman et al (1999) showed that translocations or substitutions are very rare events in this material. Therefore, difficulties in stabilising a male-fertile line were expected, especially as the restoration seemed to be the result of a monosomic addition of one chromosome from A. thaliana.…”

Section: Discussionmentioning

confidence: 97%

Arabidopsis thaliana chromosome III restores fertility in a cytoplasmic male-sterile Brassica napus line with A. thaliana mitochondrial DNA

et al. 2004

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Somatic Brassica napus (+) Arabidopsis thaliana hybrids with a cytoplasmic male sterility (CMS)-inducing cytoplasm were screened for fertility-restored plants. One line was selected and recurrently backcrossed with the maintainer line, B. napus, resulting in fertile/sterile segregating populations. Restriction fragment length polymorphism mapping showed the co-segregation of A. thaliana chromosome (chr) III markers with the fertility trait. As it was not possible to stabilise the fertility trait via selfings, a dihaploidisation strategy was assessed. Ninety haploid plants were regenerated and analysed with numerous simple sequence length polymorphism (SSLP) markers. Markers covering both arms of A. thaliana chr III were present in two plants, whereas no A. thaliana DNA could be detected in the other plants. Following colchicine-induced chromosome doubling only these two plants with A. thaliana DNA produced fertile offspring. In one of the two lines, however, the A. thaliana-specific DNA markers and fertility were lost in subsequent generations. The other line remained fertile after repeated selfings. Using genomic in situ hybridisation (GISH) we were able to demonstrate that this latter line possessed a disomic addition of the A. thaliana chromosome. The restored line was comparable to the maintainer line with respect to flower morphology, but the petals and stamens were slightly reduced in size. The homeotic conversion of stamens to pistil-like structures, which is typical for the CMS line, was reversed, and stamens with a normal appearance with viable pollen appeared. Flowering time was as in the CMS line-in both lines it was delayed in comparison to the maintainer line. The introgressed chromosome also contributes to several pleiotropic effects, such as reduced leaf crinkling and shorter stems. The ability to restore fertility through the introgression of nuclear genes from the main cytoplasmic donor species indicates that the CMS trait in this system mainly is due to B. napus/ A. thaliana alloplasmic incompatibility and not mitochondrial DNA rearrangements. Further exploitation of the material is discussed.

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Inheritance of Arabidopsis DNA in offspring from Brassica napus and A. thaliana somatic hybrids

Cited by 13 publications

References 27 publications

Brassica napus lines with rearranged Arabidopsis mitochondria display CMS and a range of developmental aberrations

Brassica napus lines with rearranged Arabidopsis mitochondria display CMS and a range of developmental aberrations

Arabidopsis thaliana-derived resistance against Leptosphaeria maculans in a Brassica napus genomic background

Arabidopsis thaliana chromosome III restores fertility in a cytoplasmic male-sterile Brassica napus line with A. thaliana mitochondrial DNA

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