Inheritance of downy mildew resistance at cotyledon and adult-plant stages in ‘Couve Algarvia’ (Brassica oleracea var. tronchuda)

Monteiro, António A.; Coelho, Paula S.; Bahćevandžiev, Kiril; Valério, Luísa

doi:10.1007/s10681-005-5696-8

Cited by 35 publications

(28 citation statements)

References 19 publications

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“…In this study, we have confirmed the resistance in lines used in our previous research in Portugal (Coelho et al 1998;Monteiro et al 2005) and the UK (Vicente et al 2012). As all tests were performed at the cotyledon stage, it will be important to test the lines at a later stage of development with a range of H. brassicae isolates, as resistance in adult leaf plants can be independent controlled from seedling resistance (Monteiro et al 2005).…”

Section: Discussionsupporting

confidence: 80%

“…As all tests were performed at the cotyledon stage, it will be important to test the lines at a later stage of development with a range of H. brassicae isolates, as resistance in adult leaf plants can be independent controlled from seedling resistance (Monteiro et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Four B. oleracea lines developed in the UK were selected based on results obtained in previous work to define different sources of seedling resistance (Vicente et al 2012), and three other lines were selected based on results of field trials performed in Portugal (Coelho et al 1998;Monteiro et al 2005) (Table 1). A rapid-cycling brassica line cv.…”

Section: Plant Materialsmentioning

confidence: 99%

“…For the present study we assembled a set of genetically uniform lines of B. oleracea (doubled haploid or self-pollinated inbred lines) derived from our previous efforts to identify sources of downy mildew resistance from European diversity of the species (Coelho et al 1998;Monteiro et al 2005;Vicente et al 2012). Our objective was to use this host differential set to characterize pathotypic variation of H. brassicae within a European sample of the pathogen collected from various crop types of B.…”

Section: Introductionmentioning

confidence: 99%

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Pathotypic diversity of Hyaloperonospora brassicae collected from Brassica oleracea

et al. 2012

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Downy mildew caused by Hyaloperonospora brassicae is an economically destructive disease of brassica crops in many growing regions throughout the world.Specialised pathogenicity of downy mildews from different Brassica species and closely related ornamental or wild relatives has been described from host range studies.Pathotypic variation amongst Hyaloperonospora brassicae isolates from Brassica oleracea has also been described; however, a standard set of B. oleracea lines that could enable reproducible classification of H. brassicae pathotypes was poorly developed. For this purpose, we examined the use of eight genetically refined host lines derived from our previous collaborative work on downy mildew resistance as a differential set to characterise pathotypes in the European population of H. brassicae. Interaction phenotypes for each combination of isolate and host line were assessed following drop inoculation of cotyledons and a spectrum of seven phenotypes was observed based on the level of sporulation on cotyledons and visible host responses. Two host lines were resistant or moderately resistant to the entire collection of isolates, and another was universally susceptible. Five lines showed differential responses to the H. brassicae isolates. A minimum of six pathotypes and five major effect resistance genes are proposed to explain all of the observed interaction phenotypes. The B. oleracea lines from this study can be useful for monitoring pathotype frequencies in H. brassicae populations in the same or other vegetable growing regions, and to assess the potential durability of disease control from different combinations of the predicted downy mildew resistance genes.3

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pathotypic diversity of Hyaloperonospora brassicae collected from Brassica oleracea

et al. 2012

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“…The resistance of mature broccoli plants having eight or more leaves is independent of the resistance of young seedlings (Dickson and Petzoldt 1993) and the plants can be susceptible at the cotyledon stage and resistant at the adult stage (Coelho and Monteiro 2003b). In 'Couve Algarvia', resistance at cotyledon and adult-plant stages is under the control of two different genetic systems (Monteiro et al 2005). Agnola et al (2003) used leaf discs collected from 17 leaf positions in 10-18 week-old Brassica plants to show that incompatible reactions occur regardless of leaf position, but with compatible reactions, sporulation intensity varies with the position of the leaf on the stem.…”

Section: Introductionmentioning

confidence: 99%

Leaf position, leaf age and plant age affect the expression of downy mildew resistance in Brassica oleracea

Coelho¹,

Valério

Monteiro

2009

Eur J Plant Pathol

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Downy mildew caused by the oomycete Hyaloperonospora parasitica (formerly Peronospora parasitica) is a worldwide foliar disease of Brassica vegetables, which may cause seedling loss in the nurseries and damage to adult plants in the field. Disease symptoms start from the lower leaves and progress upwards. Three experiments were conducted under controlled environment conditions, using inoculated leaf discs, to determine the influence of leaf position, plant age, and leaf age on the expression of resistance to downy mildew in various Brassica oleracea genotypes. The upper leaves were more resistant than the lower leaves when 7-19 week-old plants of broccoli and Tronchuda cabbage were tested. Broccoli lines 'PCB21.32' and 'OL87123-2' were fully susceptible at the cotyledon stage, showed a clear resistance increase from lower to upper leaves at 6 weeks and 'PCB21.32' was fully resistant 16 weeks after sowing. Immature leaves were more resistant than adjacent fully expanded mature leaves. Susceptibility increased with leaf age when the same leaf was tested at two to 4-week intervals. Leaf age and upper-leaf position on the stem had opposite effects on disease score, since younger leaves collected from lower positions and older leaves collected from upper positions tended to score similarly in compatible interactions. The progression of downy mildew from the base of the plant upwards on B. oleracea in the field could be due to differences in leaf resistance in addition to environmental variation. To maximise the expression of a compatible reaction in adult plants lower leaves of Brassica plants that are at least 12 weeks-old should be used.

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Downy mildew resistance and genetic variability in a wild rocket germplasm collection

et al. 2022

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One hundred accessions of a "core collection" of Diplotaxis tenuifolia (L.) DC. and Eruca spp. were screened at seedling stage for resistance to downy mildew. Accessions tested at the seedling stage were assigned to 0-6 interaction phenotypes. All cultivated rocket (Eruca spp.) accessions exhibited a resistant (R) response both in cotyledons and in young leaves. The wild rocket (D. tenuifolia) accessions exhibited higher susceptibility in cotyledons than in the 1st and 2nd leaves, with 16 and 47 accessions classified as resistant or partially resistant (PR) in the cotyledon and in leaves stages, respectively. Only three wild rocket accessions displayed an R phenotype in cotyledons and leaves. The most frequent response in cotyledons vs. leaves was the highly susceptible/susceptible (HS/S) combination (33 accessions), followed by the S/PR combination (18 accessions). A significant correlation (r = 0.917, P < .000) was observed between the disease index in cotyledons and leaves. The molecular markers analyses revealed a wide genetic distance between Diplotaxis and Eruca, which gather in two clearly separated species clusters. The molecular variability is accompanied by a wide diversity of interactions with the pathogen isolate. The closest similarities among D. tenuifolia accessions were found in accessions provided by the same breeding company. Future studies will be focused on two main objectives: (a) the assessment of the accessions behavior that have evidenced an R/R, S/PR, and HS/PR cotyledon and leaf response under greenhouse or field production and (b) the genome mapping of genetic features that provide downy mildew resistance.

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Inheritance of downy mildew resistance at cotyledon and adult-plant stages in ‘Couve Algarvia’ (Brassica oleracea var. tronchuda)

Cited by 35 publications

References 19 publications

Pathotypic diversity of Hyaloperonospora brassicae collected from Brassica oleracea

Pathotypic diversity of Hyaloperonospora brassicae collected from Brassica oleracea

Leaf position, leaf age and plant age affect the expression of downy mildew resistance in Brassica oleracea

Downy mildew resistance and genetic variability in a wild rocket germplasm collection

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